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Commit 4c68f67b authored by Oliver Loreto's avatar Oliver Loreto
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Update wiki/pages/results.html

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{% block page_content %}
<div class="row mt-4">
<h1>Wetlab Summary</h1>
<p></p>
<hr></hr>
<h2>Future Steps</h2>
<p></p>
<hr></hr>
<h1>Computational Summary</h1>
<p>There are many biomarkers associated with concussions and mTBI, but their binding interactions with particular proteins are not often studied so some forms of binding (particularly the strength of the biomarker/binder complex) are difficult to characterize using only the literature. After narrowing the field of likely biomarkers and binders from our literature search, we obtained sequences and then used AlphaFold<sup><a href = "#Ref1">1</a></sup> to model complexes between binders and biomarkers. Based on the results from alphafold, we also determined binding effectiveness with two different docking suites, HDOCK and ZDOCK. Based on the results of all these simulations, we decided to use UCHL1 as our biomarker and split ubiquitin as our binder. GFAP had some promising binders as well, but we ultimately decided to focus purely on UCHL1 since we were unable to express GFAP.</p>
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