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Commit 7439514f authored by Vitor Frost Marchesan's avatar Vitor Frost Marchesan
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minor fixes to the text

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<p style="text-align:justify">To simulate real production of the engineered cell and optimize throughput, most
labs test those cells on Bench-top bioreactors capable of measuring online data, simulate environment
conditions, <em>e.g.</em>, fed batch or pH control. Sometimes, those labs do not have enough resources to
acquire equipment these systems.</p>
acquire these systems.</p>
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<h2 id="HARDWARE: Open-Source Modular Bioreactor">HARDWARE: Open-Source Modular Bioreactor</h2>
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<p style="text-align:justify">Unfortunately, due to lack of time and geographical distance <em>-Vitor Marchesan, who assembled the Hardware, lives in a different city from where ProChi WetLabs are located, we are 600km apart-</em>, we were unable to test the bioreactor with our genetically modified <i>Lactobacillus acidophilus</i>. However, we were able to test the bioreactor functionalities with another microorganisms, obtained from partner laboratory in his University.
<p>Experiments were made in triplicate, using the microalgae <em>Chlamydomonas reinhardtii</em>, since it is a model organism. Those tests were performed in a room with always on air-conditioning set to 25&deg;C. The strain used was a wildtype one CC1690 and the reactor configuration was a single batch PBR with 500mL working volume. Strain was grown in Erlenmeyer flasks containing 100 mL of TAP media [2] on an
orbital shaker at 110 RPM, and under constant light (100 &mu;mol photons.m<sup>-2</sup>.s<sup>-1</sup>) for 3
days at room temperature, in which an inoculation of 5mL of this culture was added to the reactor containing
500mL of TAP. A sample was daily taken from days zero to five, and an extra sample was taken on day 7 to verify
reactor stability. Results are shown below:</p>
<p>Experiments were made in triplicate, using the microalgae <em>Chlamydomonas reinhardtii</em>, since it is a model organism. Those tests were performed in a room with always on air-conditioning set to 25&deg;C. The strain used was a wildtype one CC1690 and the reactor configuration was a single batch PBR with 500mL working volume. Strain was grown in Erlenmeyer flasks containing 100 mL of TAP media [2] on an orbital shaker at 110 RPM, and under constant light (100 &mu;mol photons.m<sup>-2</sup>.s<sup>-1</sup>) for 3 days at room temperature, in which an inoculation of 5mL of this culture was added to the reactor containing 500mL of TAP. A sample was daily taken from days zero to five, and an extra sample was taken on day 7 to verify reactor stability. Results are shown below:</p>
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