<pstyle="text-align:justify">Unfortunately, due to lack of time and geographical distance <em>-Vitor Marchesan, who assembled the Hardware, lives in a different city from where ProChi WetLabs are located, we are 600km apart-</em>, we were unable to test the bioreactor with our genetically modified <i>Lactobacillus acidophilus</i>. However, we were able to test the bioreactor functionalities with another microorganisms, obtained from partner laboratory in his University.
<p>Experiments were made in triplicate, using the microalgae <em>Chlamydomonas reinhardtii</em>, since it is a model organism. Those tests were performed in a room with always on air-conditioning set to 25°C. The strain used was a wildtype one CC1690 and the reactor configuration was a single batch PBR with 500mL working volume. Strain was grown in Erlenmeyer flasks containing 100 mL of TAP media [2] on an
orbital shaker at 110 RPM, and under constant light (100 μmol photons.m<sup>-2</sup>.s<sup>-1</sup>) for 3
days at room temperature, in which an inoculation of 5mL of this culture was added to the reactor containing
500mL of TAP. A sample was daily taken from days zero to five, and an extra sample was taken on day 7 to verify
reactor stability. Results are shown below:</p>
<p>Experiments were made in triplicate, using the microalgae <em>Chlamydomonas reinhardtii</em>, since it is a model organism. Those tests were performed in a room with always on air-conditioning set to 25°C. The strain used was a wildtype one CC1690 and the reactor configuration was a single batch PBR with 500mL working volume. Strain was grown in Erlenmeyer flasks containing 100 mL of TAP media [2] on an orbital shaker at 110 RPM, and under constant light (100 μmol photons.m<sup>-2</sup>.s<sup>-1</sup>) for 3 days at room temperature, in which an inoculation of 5mL of this culture was added to the reactor containing 500mL of TAP. A sample was daily taken from days zero to five, and an extra sample was taken on day 7 to verify reactor stability. Results are shown below:</p>