The strong promoter sequences we constructed could be used to up-regulate hazardous genes in pathogenic E. coli. Also, we will be working primarily with liquids, which are all non-hazardous, but could nonetheless be splashed in a team member's face or eyes. There will also be a fire hazard when conducting sterile work.
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Our E. coli are lab strains and do not have the adaptations required to survive outside a controlled environment.
The strong promoter sequences we constructed could be used to up-regulate hazardous genes in pathogenic E. coli. Also, we will be working primarily with liquids, which are all non-hazardous, but could nonetheless be splashed in a team member's face or eyes. There will also be a fire hazard when conducting sterile work. Our E. coli are lab strains and do not have the adaptations required to survive outside a controlled environment.
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Jim Baugh, Olga Draper, Krystyna Kozakiewicz
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Biosafety officers at UC Berkeley
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https://ehs.berkeley.edu/topics/lab-safety
<ahref="https://ehs.berkeley.edu/topics/lab-safety">Lab Safety Training by the Berkeley Office of Environment, Health & Safety</a>.