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Commit 450cf8cf authored by kim1339's avatar kim1339
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polished entrepreneurship + etc.

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wiki/menu.html
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</a>
<ul class="dropdown-menu" aria-labelledby="navbarDropdown">
<li><a class="dropdown-item" href="{{ url_for('pages', page='human-practices') }}">Integrated HP</a></li>
<li><a class="dropdown-item" href="{{ url_for('pages', page='communication') }}">Education & Communication</a></li>
<li><a class="dropdown-item" href="{{ url_for('pages', page='communication') }}">Science Communication</a></li>
<li><a class="dropdown-item" href="{{ url_for('pages', page='education') }}">Education</a></li>
<li><a class="dropdown-item" href="{{ url_for('pages', page='collaborations') }}">Collaborations</a></li>
</ul>
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wiki/pages/attributions.html
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<p><span id="category">Graphic Design & Videography:</span><br> Meriwether Morris, Janella Schwab, Max Yang</p>
<p><span id="category">Advisors:</span><br> Kevin Ao, Laura Gonazalez Campos, Ian Coccimiglio, Sibyl Drissler, Evan Gibbard, Kieran Maheden, Rodrigo Vallejos, Abishek Wadhwa</p>
<p><span id="category">Principal Investigators:</span><br> Dr. Steven Hallam, Dr. David Oliver</p>
<p><span id="category">Other Student Support:</span><br> Helia Jafari, Myles Osenton, Michaela Samanta</p>
<p><span id="category">Other Student Support:</span><br> Helia Jafari, Myles Osenton, Michaela Samanta, Aden Chan</p>
<p><span id="category">Other Advisor Support:</span><br> Emilia Chen, Madina Kagieva, Samuel King, Athanasios Kritharis, Sarah Ng, Ariel Qi, Cecilia Yang</p>
<br>
<h2 id="ExternalExpertise">External Expertise</h2>
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wiki/pages/entrepreneurship.html
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wiki/pages/index.html
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<style>
html, body {
max-width: 100%;
overflow-x: hidden;
}
.title {
text-align: center;
color: #FFFFFF;
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<button onclick="returnToTop()" id="goToTopBtn"><svg style="color: #ffffff; font-size: 3rem; display: block; text-align: right;" aria-hidden="true" focusable="false" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg></button>
<div class="indexBannerVideo">
<iframe title="UBC_Vancouver: Synaestivum Intro (2022) [English]" src="https://video.igem.org/videos/embed/2d3c057d-3e33-44a1-b4f7-b8a523e3e701?loop=1&amp;autoplay=1&amp;muted=1&amp;title=0&amp;warningTitle=0&amp;controlBar=0&amp;peertubeLink=0&amp;p2p=0" allowfullscreen="" sandbox="allow-same-origin allow-scripts allow-popups" width="560" height="315" frameborder="0"></iframe>
<iframe title="UBC_Vancouver: Synaestivum Intro (2022) [English]" src="https://video.igem.org/videos/embed/2d3c057d-3e33-44a1-b4f7-b8a523e3e701?loop=1&amp;autoplay=1&amp;muted=1&amp;title=0&amp;warningTitle=0&amp;controlBar=0&amp;peertubeLink=0&amp;p2p=0" allowfullscreen="" sandbox="allow-same-origin allow-scripts allow-popups" width="100%" height="315" frameborder="0"></iframe>
</div>
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<br><br>
<div>
<h1 id="index-tagline">Increasing wheat's tolerance to rising global temperatures</h1>
<h1 id="index-tagline">Increasing wheats' tolerance to rising global temperatures</h1>
</div>
<br><br>
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<div class="col-5" id="index-content">
<p>
In 2021 Canada had the lowest wheat crop yield since 2007<span id="highlight"><sup>[1]</sup></span>. The crop yield from 2021 compared to 2020 was down
In 2021, Canada had the lowest wheat crop yield since 2007<span id="highlight"><sup>[1]</sup></span>. The crop yield from 2021 compared to 2020 was down
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<div class="flex-child" id="value1">0</div>
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<li>Increase the rate of photosynthesis (<span id="highlight">SBPase</span>)</li>
<li>Decrease the plant stress hormone ethylene (<span id="highlight">ACC deaminase</span>)</li>
<li>Maintain protein folding (<span id="highlight">choline monooxygenase</span>)</li>
<li>All three enzymes are activated by a <span id="highlight">heat-inducible promoter</span>.</li>
</ol>
<p>All three enzymes will be activated by a <span id="highlight">heat-inducible promoter.</p>
</div>
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<div class="col-6"></div>
<div class="col-5" id="index-content">
<p>Our team also <a href="https://2022.igem.wiki/ubc-vancouver/model#modelling">mathematically modelled</a> heat-inducible gene expression and enzyme kinetics, with a focus on the ACC deaminase pathway. Our Dry-Lab team also conducted a <a href="https://2022.igem.wiki/ubc-vancouver/model#bioinformatics">bioinformatics RNA-seq analysis</a> to identify differentially expressed genes and pathways in heat and drought-stressed wheat.</p>
<p>Our team also <a href="https://2022.igem.wiki/ubc-vancouver/model#MathModels">mathematically modelled</a> heat-inducible gene expression and enzyme kinetics, with a focus on the ACC deaminase and SBPase pathways.<br><br>Our Dry-Lab team also conducted a <a href="https://2022.igem.wiki/ubc-vancouver/model#bioinformatics">bioinformatics RNA-seq analysis</a> to identify differentially expressed genes and pathways in heat and drought-stressed wheat.</p>
</div>
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<div class="row">
<div class="col-1"></div>
<div class="col-5" id="index-content">
<p>Our <a href="https://2022.igem.wiki/ubc-vancouver/human-practices">Integrated Human Practices</a> team <span id="highlight">interviewed farmers and visited wheat farms</span> to hear from our potential users about the problems they are facing with increasing temperatures. Our team took on initiatives to <a href="https://2022.igem.wiki/ubc-vancouver/communication">communicate</a> <span id="highlight">with the public about our project and synthetic biology</span> through <span id="highlight">expert panels</span>, a <span id="highlight">podcast</span> and <span id="highlight">volunteering in our community</span>.</p>
<p>Our <a href="https://2022.igem.wiki/ubc-vancouver/human-practices">Integrated Human Practices</a> team <span id="highlight">interviewed farmers and visited wheat farms</span> to hear from our potential users about the problems they are facing with increasing temperatures.<br><br>Our team also took on initiatives to <a href="https://2022.igem.wiki/ubc-vancouver/communication">communicate</a> <span id="highlight">with the public about our project and synthetic biology</span> through <span id="highlight">expert panels</span>, a <span id="highlight">podcast</span>, and <span id="highlight">volunteering in our community</span>.</p>
</div>
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{% include 'footer.html' %}
<!-- Wiki Tools: Teams are allowed to remove it -->
{% include 'wiki-tools.html' %}
<!-- {% include 'wiki-tools.html' %} -->
<!-- Bootstrap Bundle with Popper -->
<script src="{{ url_for('static', filename = 'bootstrap.bundle.min.js') }}"></script>
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wiki/pages/model.html
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<figure>
<img src="https://static.igem.wiki/teams/4296/wiki/drylab/quadratic.png" width="70%">
<figcaption>A poorly fitting quadratic function. Additional temperatures need to be tested to increase resolution of the data.</figcaption>
<!-- <br>
<br>
<img src="https://static.igem.wiki/teams/4296/wiki/drylab/gaussian.png" width="70%">
<figcaption>The Gaussian function is predicting the ideal domain needed to fit a parabolic function. Future experiments should collect temperature readings between 20&deg;C and 50&deg;C</figcaption> -->
<figcaption>The Gaussian function is predicting the ideal domain needed to fit a parabolic function. Future experiments should collect temperature readings between 20&deg;C and 50&deg;C.</figcaption>
</figure>
<br>
<p>For full equation derivations, MATLAB Simbiology files, and further details, please refer to this central <a href="https://github.com/UBC-iGEM/mathematical-modelling-2022" target="_blank">GitHub repository</a> where we have uploaded all of our mathematical modelling work and documentation to allow for exploration and reproducibility.</p>
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<br>
<p>We decided to test this circuit using light from an LED in order to ensure the circuit's functionality. The following plot was produced:</p>
<figure>
<img src="https://static.igem.wiki/teams/4296/wiki/drylab/image43.png" width="30%" style="border: none;">
<img src="https://static.igem.wiki/teams/4296/wiki/voltage-response-plot.png" width="70%" style="border: none;">
<figcaption>Voltage response of the device to light perturbation over time. The first initial down trend was the measured voltage response to increasing the light intensity of an LED; after which, we completely turned off the LED to which the voltage flattened. Subsequently, we completely turned on the LED to full power and saw the voltage response go to a constant drop. Lastly, we slowly decreased the power and saw a final upward trend.</figcaption>
</figure>
<br>
<p>After <span id="highlight">validating and demonstrating a proof of concept for our device</span>, the last step in our design process was to <span id="highlight">produce a production copy of our device on a printed circuit board (PCB)</span>. This would allow for high-throughput manufacturing as well as a more compact fit within the hardware chassis that we’ve also designed. Using KiCAD, we <span id="highlight">generated the manufacturing gerber files</span>, the files necessary to print this circuit board, and have ordered it. Unfortunately, due to the current supply chain issue, we have not yet had the opportunity to test the PCB.</p>
<figure>
<img src="https://static.igem.wiki/teams/4296/wiki/drylab/image43.png" width="40%" style="border: none;">
<figcaption>Our Printed Circuit Board (PCB)</figcaption>
</figure>
<br>
<p><b><u>Physical Device:</u></b></p>
<p><u>A) Cardboard Prototype</u></p>
<p>To create a device with sufficient dimensions to house all the hardware, we set out to 3D print a hardware piece that would fulfill this goal. We began by fitting our circuit components in a simple cardboard model. This fitting is to <span id="highlight">test how we wanted the device to assemble so cardboard was used as a very cheap, accessible, and simple prototyping build</span>. Any material can be used for this intermediary step. Two cardboard prototypes were made of 10 cm and 13 cm cubes. Smaller details such as ports were drawn in for positioning. As the circuit components fit snugly within the 10cm cube, the CAD design proceeded with those dimensions. This is to ensure that the final design is simple and portable for field use in the future. Our cardboard model allowed us to define the design specifications for our 3D printed model, therefore our next step of prototyping was to make the CAD design.</p>
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