<p>In the fifth run of making protoplasts we successfully made many protoplasts! We made protoplasts of good quality and in sufficient amounts that we could proceed to use them in transfection experiments.</p>
<figure>
<imgsrc="https://static.igem.wiki/teams/4296/wiki/images/fifth-protoplast.png"alt="Fifth round of protoplast isolation">
<imgsrc="https://static.igem.wiki/teams/4296/wiki/images/fifth-protoplast.jpeg"alt="Fifth round of protoplast isolation">
<figcaption>Photo of protoplasts isolated from the fifth trial of trying to isolate protoplasts. Many intact protoplasts are visible and some cells have been sheared.</figcaption>