<li>We introduced our project to Dr. Mingxing Tang and he was very optimistic about the preliminary design of our experiment. He suggested that codon optimization can improve translation efficiency, so as to improve the production of glutathione.</li>
<li>After we listened to the suggestions provided by Dr. Mingxing Tang, we decided to carry out codon optimization to increase the production of glutathione. This helped us to analyze and improve the experimental protocol.</li>
<li>He suggested removing the LVA tage from pDawn (cl-LVA) to reduce the background expression of the lysis gene and improve the stability of the blue light responsive system.</li>
<li>Based on the above suggestion, we found through a series of experiments that strains containing the modified plasmids grown to high concentrations under blue light on were able to lyse.</li>
<li>He also suggested removing the LVA tage from pDawn (cl-LVA) to reduce the background expression of the lysis gene and improve the stability of the blue light responsive system.</li>
<li>Based on the above suggestions, we found through a series of experiments that strains containing the modified plasmids grown to high concentrations under blue light on were able to lyse.</li>
