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<h2 class="animate wiki-title2" data-animate="zoomIn" >Prototype</h2>
<p>See <a href="https://2022.igem.wiki/szpt-china/proof-of-concept" target='_blank'>proof of concept</a> for details.</p>
<h2 class="animate wiki-title2" data-animate="zoomIn" >References</h2>
<p style="text-align: left; font-style: italic;">[1] <a href="https://2021.igem.org/Team:SZPT-CHINA/Design" target='_blank'>https://2021.igem.org/Team:SZPT-CHINA/Design</a><br>[2] Li, W., Li, Z., Yang, J. &amp; Ye, Q. Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli. <em>J. Biotechnol.</em><strong>154</strong>, 261&ndash;268 (2011).<br>[3] Teh, M. Y. <em>et al.</em>An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae. <em>Acs Synth. Biol.</em><strong>8</strong>, 708&ndash;723 (2019).<br>[4] Pophaly, S. D. <em>et al.</em>Glutathione biosynthesis and activity of dependent enzymes in food-grade lactic acid bacteria harbouring multidomain bifunctional fusion gene (gshF). <em>J. Appl. Microbiol.</em><strong>123</strong>, 194&ndash;203 (2017).<br>[5] Ryu, M.-H. &amp; Gomelsky, M. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications. <em>ACS Synth. Biol.</em><strong>3</strong>, 802&ndash;810 (2014).<br>[6] Florea, M. <em>et al.</em>Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. <em>Proc. Natl. Acad. Sci. U. S. A.</em><strong>113</strong>, E3431&ndash;E3440 (2016).<br>[7] Ohlendorf, R., Vidavski, R. R., Eldar, A., Moffat, K. &amp; M&ouml;glich, A. From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. <em>J. Mol. Biol.</em><strong>416</strong>, 534&ndash;542 (2012).<br>[8] Witte, A., Wanner, G., Sulzner, M. &amp; Lubitz, W. Dynamics of PhiX174 protein E-mediated lysis of Escherichia coli. <em>Arch. Microbiol.</em><strong>157</strong>, 381&ndash;8 (1992).<br>[9] Ceyssens, P.-J. <em>et al.</em>Genomic analysis of Pseudomonas aeruginosa phages LKD16 and LKA1: establishment of the phiKMV subgroup within the T7 supergroup. <em>J. Bacteriol.</em><strong>188</strong>, 6924&ndash;31 (2006).<br>[10] Guiziou, S. <em>et al.</em>A part toolbox to tune genetic expression in Bacillus subtilis. <em>Nucleic Acids Res.</em><strong>44</strong>, 7495&ndash;7508 (2016).<br>[11] Stirling, F. <em>et al.</em>Rational Design of Evolutionarily Stable Microbial Kill Switches. <em>Mol. Cell</em><strong>68</strong>, 686-697.e3 (2017).</p>
<p style="text-align: left; font-style: italic;">[1] <a href="https://2021.igem.org/Team:SZPT-CHINA/Design" target='_blank'>https://2021.igem.org/Team:SZPT-CHINA/Design</a><br>[2] Li, W., Li, Z., Yang, J. &amp; Ye, Q. Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli. <em>J. Biotechnol.</em><strong>154</strong>, 261&ndash;268 (2011).<br>[3] Teh, M. Y. <em>et al.</em>An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae. <em>Acs Synth. Biol.</em><strong>8</strong>, 708&ndash;723 (2019).<br>[4] Pophaly, S. D. <em>et al.</em>Glutathione biosynthesis and activity of dependent enzymes in food-grade lactic acid bacteria harbouring multidomain bifunctional fusion gene (gshF). <em>J. Appl. Microbiol.</em><strong>123</strong>, 194&ndash;203 (2017).<br>[5] Ryu, M.-H. &amp; Gomelsky, M. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications. <em>ACS Synth. Biol.</em><strong>3</strong>, 802&ndash;810 (2014).<br>[6] Florea, M. <em>et al.</em>Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. <em>Proc. Natl. Acad. Sci. U. S. A.</em><strong>113</strong>, E3431&ndash;E3440 (2016).<br>[7] Ohlendorf, R., Vidavski, R. R., Eldar, A., Moffat, K. &amp; M&ouml;glich, A. From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. <em>J. Mol. Biol.</em><strong>416</strong>, 534&ndash;542 (2012).<br>[8] Witte, A., Wanner, G., Sulzner, M. &amp; Lubitz, W. Dynamics of PhiX174 protein E-mediated lysis of Escherichia coli. <em>Arch. Microbiol.</em><strong>157</strong>, 381&ndash;8 (1992).<br>[9] Ceyssens, P.-J. <em>et al.</em>Genomic analysis of Pseudomonas aeruginosa phages LKD16 and LKA1: establishment of the phiKMV subgroup within the T7 supergroup. <em>J. Bacteriol.</em><strong>188</strong>, 6924&ndash;31 (2006).<br>[10] Guiziou, S. <em>et al.</em>A part toolbox to tune genetic expression in Bacillus subtilis. <em>Nucleic Acids Res.</em><strong>44</strong>, 7495&ndash;7508 (2016).<br>[11] Stirling, F. <em>et al.</em>Rational Design of Evolutionarily Stable Microbial Kill Switches. <em>Mol. Cell</em><strong>68</strong>, 686-697.e3 (2017).</p>
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