<pclass="content">To generate stable cell lines producing both green and red fluorescent signals, we first generated two SB donor plasmids carrying either a P<sub>RPBSA</sub>-dtomato-2A-BlastR or a P<sub>RPBSA</sub>-EGFP-2A-PuroR transposon. HEK-293T cells were co-transfected with both donor plasmids and the transposes expressing P<sub>hCMV</sub>-SB100X plasmid in a 10:10:1 ratio. Cells were treated with Blasticidin (10 <p>µ</p>g/mL) and puromycin (0.5 <p>µ</p>g/mL) since the 8<sup>th</sup> h post transfection.
<pclass="content">To generate stable cell lines producing both green and red fluorescent signals, we first generated two SB donor plasmids carrying either a P<sub>RPBSA</sub>-dtomato-2A-BlastR or a P<sub>RPBSA</sub>-EGFP-2A-PuroR transposon. HEK-293T cells were co-transfected with both donor plasmids and the transposes expressing P<sub>hCMV</sub>-SB100X plasmid in a 10:10:1 ratio. Cells were treated with Blasticidin (10 ug/mL) and puromycin (0.5 ug/mL) since the 8<sup>th</sup> h post transfection.
</p>
<pclass="content">Fluorescent imaging of the stable cell line shows a unanimous expression of EGFP and dTomato. A high heterogeneity in regards of the EGFP/dTomato ratio among the stably transfected cells can also be observed.
