<li>The bacterial solution was centrifuged with 7000-8000 rpm 2 min, and the supernatant was taken and re-suspended with appropriate amount of M9 buffer (see attached table for formula).</li>
<li>Repeat step 3 1 to 2 times for washing precipitation.</li>
<li>To prepare the working solution of 40 ml electrolytic cell: add 2 ml LB culture solution to the bacterial liquid obtained from the last re-suspension, fill the volume to 40 ml with M9, add sodium lactate to 19 mmol/L (40 ml additive 85mg ammonium lactate), and use nitrogen 15min before use to remove dissolved oxygen.</li>
<li>Set up a three-electrode test device and prepare four different electrodes (as described in the following table), in which the experimental group needs to add silver nitrate (1 mmol/L) to the working solution.</li>
<li>Set up a three-electrode test device and prepare four different electrodes, in which the experimental group needs to add silver nitrate (1 mmol/L) to the working solution.</li>
<li>The current intensity was measured at a constant voltage of 0.2 V and the I-t curve was recorded.</li>