<article><p>Biosafety and security awareness run through our projects. We have researched relevant laws and regulations, policies, conventions and other contents in the two directions of microorganisms and cells respectively. We also attended a biosafety meeting with other teams in Freshwater Bioremediation Alliance to discuss biosafety considerations and suggestions for improvement. We carefully analyzed the possible biosafety risks in our project and put forward corresponding solutions based on the collected materials.Finally, combined with the test data of our laboratory and collaborator companies, we confirm that our project has no major biosafety risks under the existing design.</p>
<article><p>Biosafety and security awareness run through our projects. We have researched relevant laws and regulations, policies, conventions and other contents in the two directions of microorganisms and cells respectively. We also attended a biosafety meeting with other teams in Freshwater Bioremediation Alliance to discuss biosafety considerations and suggestions for improvement. We carefully analyzed the possible biosafety risks in our project and put forward corresponding solutions based on the collected materials.Finally, combined with the test data of our laboratory and collaborator companies, we confirm that our project has no major biosafety risks under the existing design.</p>
<p>For strains in the MFC containment vessel, a conventional sterilisation procedure is applied directly, e.g. UV irradiation or alcohol dunking. If there are leaks the bacteria are handed over to downstream disposal.</p>
<p>For elemental silver, we need to work only on the utilisation of the silver-containing wastewater, fixing a part of the silver and subsequently treating the lost part of the nano-silver and silver ions by other processes.</p>
<p>A combined process of "three-stage chemical coagulation + ion exchange" is used to treat silver-containing wastewater. In the secondary chemical coagulation system, the pH of the wastewater is again adjusted to 11, and \(\ce{Ag+}\) and TP are further removed by the addition of \(\ce{CaCl2}\), while the removal of \(\ce{Ag+}\) is enhanced by the addition of a metal trap. In the tertiary chemical coagulation system, the pH of the wastewater is adjusted to 9-10 and the residual dissolved \(\ce{Ca^{2+} }\) is removed by the addition of \(\ce{Na2Co3}\). The effluent from the three-stage chemical coagulation system is filtered into the ion exchange system for deep silver removal, so that the \(\ce{Ag+}\) in the effluent is stabilised at < 0.1 mg/L, thus achieving the simultaneous deep removal of silver and phosphorus</p>
<p>A combined process of "three-stage chemical coagulation + ion exchange" is used to treat silver-containing wastewater. In the secondary chemical coagulation system, the pH of the wastewater is again adjusted to 11, and \(\ce{Ag+}\) and TP are further removed by the addition of \(\ce{CaCl2}\), while the removal of \(\ce{Ag+}\) is enhanced by the addition of a metal trap. In the tertiary chemical coagulation system, the pH of the wastewater is adjusted to 9-10 and the residual dissolved \(\ce{Ca^{2+} }\) is removed by the addition of \(\ce{Na2CO3}\). The effluent from the three-stage chemical coagulation system is filtered into the ion exchange system for deep silver removal, so that the \(\ce{Ag+}\) in the effluent is stabilised at < 0.1 mg/L, thus achieving the simultaneous deep removal of silver and phosphorus</p>
<h2id="validating">Validating</h2>
<p>During our communication with <ahref="https://2022.igem.wiki/nanjing-china/human-practices#heading-7">LOTUS</a>, we agreed on a preliminary <ahref="https://2022.igem.wiki/nanjing-china/implementation">implementation</a> plan. We talked about biosecurity aspects. Since the Shewanella we use is widespread in soil and oceans, it has also been verified in our laboratory to not produce substances that pose a biosecurity risk. The LOTUS laboratory has verified that if the bacteria and materials enter the downstream device with the water flow, they will also be adsorbed by a small amount of sludge and eventually incinerated, and will not leak into the environment. Therefore it is safe.</p>
<h2id="references">References</h2>
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<li><p>Law of the People's Republic of China on the Prevention and Control of Soil Pollution: <ahref="http://www.npc.gov.cn/zgrdw/npc/lfzt/rlyw/2018-08/31/content_2060840.htm">link</a></p></li>
<li><p>Regulations on Biosafety Management of Pathogenic Microbiology Laboratories: <ahref="http://wjw.jiangsu.gov.cn/art/2020/9/11/art_80250_9502309.html">link</a></p></li>
<li><p>Wang, J.-W. et al. A Self-Driven Bioreactor Based on Bacterium-Metal-Organic Framework Biohybrids for Boosting Chemotherapy via Cyclic Lactate Catabolism. <em>ACS Nano</em> (2021) doi:10.1021/acsnano.1c06123.</p></li>
<li><p>Jiang, M., Zheng, X. & Chen, Y. Enhancement of denitrification performance with reduction of nitrite accumulation and N2O emission by <em>Shewanella</em> oneidensis MR-1 in microbial denitrifying process. <em>Water Res</em><strong>169</strong>, 115242 (2020).</p></li>
<li><p>Jiang, M., Zheng, X. & Chen, Y. Enhancement of denitrification performance with reduction of nitrite accumulation and \(\ce{N2O}\) emission by <em>Shewanella oneidensis</em> MR-1 in microbial denitrifying process. <em>Water Res</em><strong>169</strong>, 115242 (2020).</p></li>
<li><p>Political Declaration of the High-Level Meeting of the General Assembly on Antimicrobial Resistance: <ahref="https://digitallibrary.un.org/record/845917">link</a></p></li>
<li><p>Convention on the Prohibition of the Development, Production and Stockpiling of Bacteriological (Biological) and Toxin Weapons and on their Destruction: <ahref="https://digitallibrary.un.org/record/179511">link</a></p></li>
<li><p>Stockholm Convention on Persistent Organic Pollutants: <ahref="https://www.un.org/zh/documents/treaty/WIPO-2001">link</a></p></li>
