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<div class="tab-pane fade show active" id="pills-overview" role="tabpanel" aria-labelledby="pills-overview-tab">
<!-- Overview tab content goes here -->
<div class="row">
<h2>Introduction</h2>
<p><b>Vibriosis</b> is a serious disease in fish, crustaceans and shellfish as recognised by the FAO, United
<h2 class="reveal-on-scroll">Introduction</h2>
<p class="reveal-on-scroll"><b>Vibriosis</b> is a serious disease in fish, crustaceans and shellfish as recognised
by the FAO, United
Nations. It
leads to <b>significant economic losses</b> and affects multiple sectors, thus hindering the development of
the
......@@ -40,24 +41,27 @@
wide range of hosts [1].
</p>
<p>Our aim through AMPifin is to <b>improve pisciculture operations</b>, and consequently increase the yield of
<p class="reveal-on-scroll">Our aim through AMPifin is to <b>improve pisciculture operations</b>, and consequently
increase the yield of
fish in
the market—a major part of various Indian states’ staple diets. This approach would immensely benefit the
economy and fisherfolk by facilitating a sustainable pisciculture industry. AMPifin is a <b>sustainable</b>
solution to
vibriosis in fish. In line with our project’s work, we carried out extensive work concerning human practices.
</p>
<h2>Problems</h2>
<h2 class="reveal-on-scroll">Problems</h2>
<p>The idea for the project provided us with a plethora of possibilities. Still, our literature research and
<p class="reveal-on-scroll">The idea for the project provided us with a plethora of possibilities. Still, our
literature research and
responsibility toward the marine populations reiterated our sense of commitment to prioritise a sustainable
approach through our solution. Nevertheless, we encountered numerous challenges during our ideation, research,
and experimentation process. We considered how our team could cushion the pisciculture industry. We knew that
for our initiative to be successful, we needed to combine and maximise the best aspects of our <b>core values,
scientific reasoning, and community involvement</b>.</p>
<h2>Solutions</h2>
<h2 class="reveal-on-scroll">Solutions</h2>
<p>During the initial stages of our project, we talked to a fish farm owner who introduced us to the disease
<p class="reveal-on-scroll">During the initial stages of our project, we talked to a fish farm owner who
introduced us to the disease
Vibriosis. We discovered that <i>Vibrio parahaemolyticus</i> is the causative bacteria. Our project’s work
began
by interacting with an expert in the field of computational biology who guided us on the engineering and
......@@ -74,9 +78,10 @@
an <b>all-inclusive demographic</b>.
</p>
<h2>Stakeholder approach</h2>
<h2 class="reveal-on-scroll">Stakeholder approach</h2>
<p>We received numerous comments and ideas after holding several meetings and conversations with <b>specialists,
<p class="reveal-on-scroll">We received numerous comments and ideas after holding several meetings and
conversations with <b>specialists,
researchers, professors, and farmers</b>. These conversations provided valuable feedback that improved the
areas we
overlooked in our project and recommended strategies to speed up its development. Without these interactions,
......@@ -95,64 +100,72 @@
about their viewpoints on the matter. The stakeholders we identified are all mentioned here, along
with an explanation of why we contacted them and what we discovered.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/human-practices/stakeholders.jpeg"
class="figure-img img-fluid rounded" alt="Stakeholder approach">
<figcaption class="figure-caption text-center">
</figcaption>
</figure>
<h3>Outreach</h3>
<p>For our integrated human practices and outreach, reaching out to the fisherfolk was crucial. We gathered
<h3 class="reveal-on-scroll">Outreach</h3>
<p class="reveal-on-scroll">For our integrated human practices and outreach, reaching out to the fisherfolk was
crucial. We gathered
information on prevalence, current solutions, and the viability of AMPifin through these visits. The visits
covered numerous locations, both national and international.</p>
<h3>Academia</h3>
<p>We sought input from professors working in the field on various techniques, including the viability of our
<h3 class="reveal-on-scroll">Academia</h3>
<p class="reveal-on-scroll">We sought input from professors working in the field on various techniques, including
the viability of our
models. Whether it came to our literature surveys or our experimental design, their overall perspectives on
our
project as subject-matter experts was extremely valuable. Furthermore, to find out the professional-scale
research being conducted on our project’s topics that were relevant to our project, we contacted members from
research institutes.</p>
<h3>Companies</h3>
<p>We contacted companies in the fields of biotechnology, synthetic biology, and aquaculture. Their
<h3 class="reveal-on-scroll">Companies</h3>
<p class="reveal-on-scroll">We contacted companies in the fields of biotechnology, synthetic biology, and
aquaculture. Their
input shaped our solution’s commercial viability.</p>
<h3>Entrepreneurs</h3>
<p>To improve our study of novel approaches such as ours, we wanted to connect with experts in entrepreneurship.
<h3 class="reveal-on-scroll">Entrepreneurs</h3>
<p class="reveal-on-scroll">To improve our study of novel approaches such as ours, we wanted to connect with
experts in entrepreneurship.
They helped us understand how we can work on the future upscaling of our project and the band of stakeholders
that would be important to us.</p>
<h3>Doctors</h3>
<p>Vibriosis is a disease that affects humans and fish, and to understand its impact in both cases, we consulted
<h3 class="reveal-on-scroll">Doctors</h3>
<p class="reveal-on-scroll">Vibriosis is a disease that affects humans and fish, and to understand its impact in
both cases, we consulted
doctors who helped us analyse the impact and medical solutions. Through these interactions, we learnt the
symptoms and treatment methods of vibriosis in humans. Additionally, we gained more information on the
pathogenicity and virulence of <i>V. parahaemolyticus</i>.</p>
<h2>Our values</h2>
<figure class="figure">
<h2 class="reveal-on-scroll">Our values</h2>
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/human-practices/three-rs.jpeg"
class="figure-img img-fluid rounded" alt="Three R's">
<figcaption class="figure-caption text-center">
</figcaption>
</figure>
<h3>Responsible</h3>
<p>As iGEM is a competition based on synthetic biology, there are several biosafety issues that could emerge.
<h3 class="reveal-on-scroll">Responsible</h3>
<p class="reveal-on-scroll">As iGEM is a competition based on synthetic biology, there are several biosafety
issues that could emerge.
Our
target organism—<i>Vibrio parahaemolyticus</i> is a Risk Group 2 organism. To avoid any sort of hazards, we
decided to not work with this organism for our experiments. Instead, we decided to
express our target molecule–MAM7 via <i>E.coli</i>.
<p>
<h3>Reflective</h3>
<p>Human practices and community engagement formed the backbone of our project. The conversations we had with
<p class="reveal-on-scroll">
<h3 class="reveal-on-scroll">Reflective</h3>
<p class="reveal-on-scroll">Human practices and community engagement formed the backbone of our project. The
conversations we had with
the
multiple
individuals helped us gain a holistic perspective of the problem. The insights and opinions helped us shape
our
project. After every interaction, our team sat together and reflected upon the content of the meetings in
order to make improvements to our solution.</p>
<h3>Responsiveness</h3>
<p>Following our session on reflection on the community engagement programmes, we implemented their
<h3 class="reveal-on-scroll">Responsiveness</h3>
<p class="reveal-on-scroll">Following our session on reflection on the community engagement programmes, we
implemented their
opinion to the best of our ability. Since this project was created to help out the
local people and the problems they face, incorporating their opinions and needs into our project was crucial.
Our project trajectory changed majorly due to their input. Public opinion is a key aspect for development of
......@@ -168,8 +181,9 @@
<div class="tab-pane fade" id="pills-engagement" role="tabpanel" aria-labelledby="pills-engagement-tab">
<!-- Engagement tab content goes here -->
<div class="row">
<h2>Overview</h2>
<p>“Local people solving local problems” is a phrase that describes AMPifin accurately. Following a
<h2 class="reveal-on-scroll">Overview</h2>
<p class="reveal-on-scroll">“Local people solving local problems” is a phrase that describes AMPifin accurately.
Following a
value-centric
approach, we noticed that Manipal being a coastal town is home to a massive aquaculture industry setup. As our
conversations with various stakeholders and communities developed, we came across <b>Mr. Kaushik Alike</b>,
......@@ -179,15 +193,17 @@
and through this interaction, we fixated on our problem statement and decided to build on it systematically.
</p>
<p>The food-chain supply for seafood consists of hatcheries, farms, markets, restaurants, and finally,
<p class="reveal-on-scroll">The food-chain supply for seafood consists of hatcheries, farms, markets, restaurants,
and finally,
consumers.
To understand the impact of vibriosis, we interacted with various stakeholders from in and around India in
Maharashtra, Karnataka, Hyderabad, Tamil Nadu and Kerala, and internationally, in Dubai. These interactions
helped augment our project and accelerated its growth.</p>
<h2>Interaction with Canares Aquaculture LPP</h2>
<h2 class="reveal-on-scroll">Interaction with Canares Aquaculture LPP</h2>
<p>On 21st November 2022, we contacted Kaushik Alike, one of the founders of Canares Aquaculture. He spoke to us
<p class="reveal-on-scroll">On 21st November 2022, we contacted Kaushik Alike, one of the founders of Canares
Aquaculture. He spoke to us
about various topics, including parasites and viruses, as well as feed and water purification. We learnt about
the major diseases in fish and the present methods of prevention and cure. He was the one who first mentioned
<i>Vibrio parahaemolyticus</i> and Oxytetracycline to us. We decided to work on the bacteria <i>Vibrio
......@@ -196,18 +212,21 @@
body. He also discussed several strategies for distributing feed and raising fish.
</p>
<h2>Government Shrimp and Fish Hatchery</h2>
<h2 class="reveal-on-scroll">Government Shrimp and Fish Hatchery</h2>
<p>To gain more knowledge of areas outside of our locality, we decided to approach a government hatchery to get
<p class="reveal-on-scroll">To gain more knowledge of areas outside of our locality, we decided to approach a
government hatchery to get
more information about the prevalence of vibriosis.</p>
<p>On 1st July 2022, we visited a Government Shrimp and Fish Hatchery in Azhikode, Kerala, India . We interacted
<p class="reveal-on-scroll">On 1st July 2022, we visited a Government Shrimp and Fish Hatchery in Azhikode,
Kerala, India . We interacted
with their association members—Assistant Fisheries Extension Officers—Mrs. Merit Kurian, Mrs. Devi Chandran,
and
Mr. Vinu Jacob. The primary focus of this institution is to hatch shrimp and fish for 20–25 days using biofloc
technology and then sell them to various fish farmers.</p>
<p>They mentioned that the main types of fish that are hatched are Etroplus and Pompano. They elaborated on the
<p class="reveal-on-scroll">They mentioned that the main types of fish that are hatched are Etroplus and Pompano.
They elaborated on the
primary local diseases observed in their farm. It astounded us to hear that there are multiple species
responsible for various levels and types of pathogenicity. The fisherfolk mostly look for cuts or bruises
found
......@@ -224,7 +243,8 @@
levels of pathogenicity and how fisherfolk differentiate between diseased and healthy fish. We
also learned more about methods by which they prevent or cure bacterial infections in fish.</p>
<p>When we explained AMPifin to them, they were interested in our solution's approach. In particular, they were
<p class="reveal-on-scroll">When we explained AMPifin to them, they were interested in our solution's approach. In
particular, they were
impressed with our focus on sustainability. In particular, they were keen on exploring a solution that has no
known side effects and is unlikely to induce antibiotic resistance. Through this visit, we comprehended the
large-scale working of fish hatcheries and developed an understanding of how AMPifin can be stitched toward
......@@ -234,7 +254,7 @@
<div class="row">
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/govt-shrimp-hatchery-1.jpg"
class="figure-img img-fluid rounded" alt="The Government Shrimp Hatchery in Kerala">
<figcaption class="figure-caption text-center">
......@@ -243,7 +263,7 @@
</figure>
</div>
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/govt-shrimp-hatchery-2.jpg"
class="figure-img img-fluid rounded" alt="Interaction at the Government Shrimp Hatchery">
<figcaption class="figure-caption text-center">
......@@ -253,13 +273,15 @@
</div>
</div>
<h2>Laxmipuri Fish Market</h2>
<h2 class="reveal-on-scroll">Laxmipuri Fish Market</h2>
<p>Fish markets are the hub for fish trade and team AMPifin decided to interact with them to understand the
<p class="reveal-on-scroll">Fish markets are the hub for fish trade and team AMPifin decided to interact with them
to understand the
food-supply chain and their methods of identification for diseased fish. Furthermore, the platform served as a
location to interact with consumers, too.</p>
<p>On 6th July 2022, we visited Laxmipuri Fish Market located in Kolhapur, Maharashtra, India. It is owned by
<p class="reveal-on-scroll">On 6th July 2022, we visited Laxmipuri Fish Market located in Kolhapur, Maharashtra,
India. It is owned by
Mr.
Ashish, Mr. Kiran, and Mr. Ashok—fish sellers and consumers from a local fish market. They mentioned that
their
......@@ -275,7 +297,7 @@
they were encouraging of more such sustainable solutions. From this visit, we understood the importance of
consulting secondary stakeholders, who are a critical part of the food supply chain.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/fish-market-1.jpg"
class="figure-img img-fluid rounded" alt="Interactions at the Laxmipuri Fish Market" width="70%">
<figcaption class="figure-caption text-center">
......@@ -283,9 +305,10 @@
</figcaption>
</figure>
<h2>Visit to Shrimp and Fish Farm</h2>
<h2 class="reveal-on-scroll">Visit to Shrimp and Fish Farm</h2>
<p>On 8th July 2022, we visited Mr. Sampath's shrimp and fish farm in Chennai, India. While showing us around
<p class="reveal-on-scroll">On 8th July 2022, we visited Mr. Sampath's shrimp and fish farm in Chennai, India.
While showing us around
his farm, he elaborated on the two main species of shrimps grown—giant Tiger prawns (<i>Penaeus monodon</i>)
and
Whiteleg shrimps (<i> Litopenaeus vannamei</i>). The salinity range maintained in his farm roughly accounts
......@@ -293,7 +316,8 @@
about 25%, along with a pH range of 8.5, and the breeding period is usually carried out for four months. He
mentioned how these conditions were applicable for fish as well.</p>
<p>For fish, any disease is detected by visual cues—cuts, scars, bleeding on the skin, and necrosis. The
<p class="reveal-on-scroll">For fish, any disease is detected by visual cues—cuts, scars, bleeding on the skin,
and necrosis. The
detection
of such diseases is carried out by testing the water with a calcium soda mix. Since
AMPifin could potentially be a broad-spectrum solution for all gram-negative pathogens, we were delighted to
......@@ -302,7 +326,7 @@
<div class="row">
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/community-shrimp-farm-2.jpg"
class="figure-img img-fluid rounded" alt="Interactions at the shrimp and fish farm in Chennai">
<figcaption class="figure-caption text-center">
......@@ -311,7 +335,7 @@
</figure>
</div>
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/community-shrimp-farm.jpg"
class="figure-img img-fluid rounded" alt="Distributing the pamphlet at the farm">
<figcaption class="figure-caption text-center">
......@@ -321,9 +345,10 @@
</div>
</div>
<h2>ICAR-CIBA Institute, Chennai, India</h2>
<h2 class="reveal-on-scroll">ICAR-CIBA Institute, Chennai, India</h2>
<p>On 8th July 2022, we visited the Indian Council of Agricultural Research—Central Institute of Brackishwater
<p class="reveal-on-scroll">On 8th July 2022, we visited the Indian Council of Agricultural Research—Central
Institute of Brackishwater
Research (ICAR-CIBA) located in Chennai. We met with Dr. M Shashi Shekar and his team consisting of Dr. Ashok,
Dr. Raymond, Dr. Sujeet and Dr. Patil who are currently working on vibriosis in fish. We felt that the
information they had would benefit our project’s geographical statistics and research-related data. There was
......@@ -335,7 +360,7 @@
<div class="row">
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/icar-ciba-offline-3.jpg"
class="figure-img img-fluid rounded" alt="Laboratory at ICAR-CIBA">
<figcaption class="figure-caption text-center">
......@@ -344,7 +369,7 @@
</figure>
</div>
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/icar-ciba-offline-1.jpg"
class="figure-img img-fluid rounded" alt="Interactions at the ICAR-CIBA Institute">
<figcaption class="figure-caption text-center">
......@@ -354,18 +379,21 @@
</div>
</div>
<h2>Al Fahlain Aquaculture and Agricultural LLC</h2>
<h2 class="reveal-on-scroll">Al Fahlain Aquaculture and Agricultural LLC</h2>
<p>We visited The Al Fahlain fish farm in Dubai, UAE, headed by Mr. Liju, to check the prevalence of vibriosis
<p class="reveal-on-scroll">We visited The Al Fahlain fish farm in Dubai, UAE, headed by Mr. Liju, to check the
prevalence of vibriosis
in locations outside of India. Initially, he exchanged data about the food they usually feed the fish, which
were mostly floating pellets that they import from Saudi Arabia and sinking pellets which are rich sources of
protein
imported from Turkey. </p>
<p>They indicated that vibriosis is a common disease in fish in that area, but due to restricted lab facilities,
<p class="reveal-on-scroll">They indicated that vibriosis is a common disease in fish in that area, but due to
restricted lab facilities,
it is not usually tested.</p>
<p>From our visit, we observed that they use antibiotics to treat these infected fish and the most commonly used
<p class="reveal-on-scroll">From our visit, we observed that they use antibiotics to treat these infected fish and
the most commonly used
one is Oxytetracycline (OTC). OTC is a broad-spectrum antibiotic and is hence helpful in most common diseases.
Erythromycin was used previously along with OTC but isn’t effective for many diseases anymore, and hence, it
has been banned in many countries, including India and Dubai. In cases where they cannot detect the
......@@ -375,17 +403,19 @@
PCR test from the country of import. This has reduced the mortality rate of fish by
20%. </p>
<p>Apart from these diseases in fish, the main cause of diseases in this fish farm was the high-density
<p class="reveal-on-scroll">Apart from these diseases in fish, the main cause of diseases in this fish farm was
the high-density
culturing of fish, due to which the oxygen level and space for each fish reduces. They have external liquid
oxygen that is usually pumped into the fish columns depending on the need and necessity. </p>
<p>This visit helped us analyse the prevalence of vibriosis outside of India as well. Furthermore, we
<p class="reveal-on-scroll">This visit helped us analyse the prevalence of vibriosis outside of India as well.
Furthermore, we
distributed large-size pamphlets on the safe usage of antibiotics, and explained AMPifin to the fish farm
owners. </p>
<div class="row">
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/igem-mit-mahe-dubai-farm-6.jpg"
class="figure-img img-fluid rounded" alt="Picture of Al Fahlain Aquaculture and Agriculture LLC">
<figcaption class="figure-caption text-center">
......@@ -394,7 +424,7 @@
</figure>
</div>
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/community-dubai.jpg"
class="figure-img img-fluid rounded"
alt="Distributing pamphlets at the Al Fahlain Aquaculture and Agriculture LLC">
......@@ -405,11 +435,12 @@
</div>
</div>
<h2>Aquatic Biosystems</h2>
<h2 class="reveal-on-scroll">Aquatic Biosystems</h2>
Initially, vibriosis started out as a local problem team AMPifin wanted to focus on. To gain a closer look into
the problem around the Mangalore-Manipal region, we visited Aquatic Biosystems.</p>
<p>We visited Aquatic Biosystems in Mangalore, owned by Mr. Ronald D’Souza. He set up Aquatic Biosystems in
<p class="reveal-on-scroll">We visited Aquatic Biosystems in Mangalore, owned by Mr. Ronald D’Souza. He set up
Aquatic Biosystems in
2006, which is currently the largest ornamental fish farm in the state. He began by talking to us about the
history and origin of his biosystem. As he showed us around his fish farm he spoke about the major diseases
they face. He mentioned that viral and bacterial infections are quite common. If there are any symptoms of
......@@ -419,7 +450,8 @@
Enrofloxacin (ENR). Of these, OTC works better than ENR. However, if OTC does not work, they use Seromycin
followed by lab testing.</p>
<p>Every year, they face a loss percentage of 5–10% due to different types of diseases. On explaining our
<p class="reveal-on-scroll">Every year, they face a loss percentage of 5–10% due to different types of diseases.
On explaining our
project to him, he was eager to see the result. He explained how the pipe fittings and the connections were
formed in order to incorporate various water filters. This paved the route for our delivery mechanism to find
an appropriate real-life application. Given the description of the Recirculatory Aquaculture System (RAS) in
......@@ -431,7 +463,7 @@
<div class="row">
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/aquatic-biosystems-5.jpg"
class="figure-img img-fluid rounded" alt="Picture of Aquatic Biosystems">
<figcaption class="figure-caption text-center">
......@@ -440,7 +472,7 @@
</figure>
</div>
<div class="col-6">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/aquatic-biosystems-outreach.jpg"
class="figure-img img-fluid rounded" alt="Distributing pamphlets at Aquatic Biosystems">
<figcaption class="figure-caption text-center">
......@@ -450,15 +482,17 @@
</div>
</div>
<h2>Thimmapa Restaurant</h2>
<h2 class="reveal-on-scroll">Thimmapa Restaurant</h2>
<p>To understand the impact of vibriosis on our secondary stakeholders—i.e., seafood restaurant chefs and
<p class="reveal-on-scroll">To understand the impact of vibriosis on our secondary stakeholders—i.e., seafood
restaurant chefs and
owners, we decided to visit a renowned restaurant in the Udupi region of Karnataka. We wanted to observe the
various procurement, cleaning, sanitisation, and cooking practices carried out by the hotel administrators.
Furthermore, we wanted to understand the impact and significance of our solution at this stage of the food
supply chain.</p>
<p>On October 3rd 2022, a few of our team members visited Hotel Thimappa—a seafood restaurant in Udupi,
<p class="reveal-on-scroll">On October 3rd 2022, a few of our team members visited Hotel Thimappa—a seafood
restaurant in Udupi,
Karnataka. We spoke with the owner about how the fish is stored and prepared at their restaurant. He explained
that the monsoon season around July to October is the best time of the year for fishing. For the past 40
years, the restaurant has purchased its fish from the same vendor located in and around the coast of Malpe.
......@@ -471,7 +505,8 @@
difference in texture and smell. However, if it is a diseased fish, it is hard to detect while cleaning. These
fish are identified during the cooking stage.</p>
<p>We introduced them to our project—AMPifin and they were inquisitive about our delivery mechanism. From this
<p class="reveal-on-scroll">We introduced them to our project—AMPifin and they were inquisitive about our delivery
mechanism. From this
visit, we understood the effects of diseased/rotten fish on different stages of consumption. We also learned
the methods of segregating rotten or diseased fish at a seafood restaurant. We
ended this visit by trying two of their famous seafood dishes. The dishes were absolutely delicious, and
......@@ -479,7 +514,7 @@
stakeholder interaction spectrum and understand the prevalence of vibriosis at various stages of the
food-supply chain.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/communication/fish-restaurant-3.jpg"
class="figure-img img-fluid rounded" alt="Photo with restaurant owner" width="40%">
<figcaption class="figure-caption text-center">
......@@ -487,8 +522,9 @@
</figcaption>
</figure>
<h2>Podcast with The Astronomy Club, Manipal.</h2>
<p>On 7th October 2022, we collaborated with the Astronomy Club, Manipal to talk about synthetic biology and
<h2 class="reveal-on-scroll">Podcast with The Astronomy Club, Manipal.</h2>
<p class="reveal-on-scroll">On 7th October 2022, we collaborated with the Astronomy Club, Manipal to talk about
synthetic biology and
its applications in the space industry. The podcast comprised two members of the Astronomy Club and two
members of Manipal BioMachines. The podcast started off with an introduction to the respective members and
iGEM. The discussion was based on how synthetic biology could be used to enhance and use the extreme
......@@ -499,22 +535,25 @@
projects that built on the motive of improving space travel quality for the travellers by integrating pure
synthetic biology based components. </p>
<p>This podcast was an opportunity for us to collaborate with astrophiles and understand their areas of
<p class="reveal-on-scroll">This podcast was an opportunity for us to collaborate with astrophiles and understand
their areas of
interest. By engaging with the astrophile community, we were able to spread the idea of synthetic biology to
various groups. Astrobiology is a vast field and has developed for itself avid learners. Through this podcast,
our aim was to introduce them to a novel field under the same branch of science. Refer to our <a
href="{{ url_for('pages', page='collaborations') }}">collaboration</a> section to listen to the podcast.</p>
<h2>Consumer Survey</h2>
<h2 class="reveal-on-scroll">Consumer Survey</h2>
<p>To understand the perception of the general public on the pisciculture industry, we rolled out an exhaustive
<p class="reveal-on-scroll">To understand the perception of the general public on the pisciculture industry, we
rolled out an exhaustive
consumer survey. This survey helped us understand the opinion of the general public on sustainable solutions
based on synthetic biology. The responses brought a broader perspective to our project, and it was interesting
to see the level of awareness amongst various seafood consumers on diseases in fish and their prevalence.
Attached
below is a detailed consolidation and summarised inference:</p>
<iframe class="align-self-center my-5" loading="lazy" height="500px" width="100%" title="survey consolidation"
<iframe class="align-self-center my-5 reveal-on-scroll" loading="lazy" height="500px" width="100%"
title="survey consolidation"
src="https://static.igem.wiki/teams/4200/wiki/pdfs/igem-mit-mahe-survey-consolidation-human-practices-community-engagement.pdf#zoom=FitW"></iframe>
</div>
......@@ -523,14 +562,16 @@
<!-- IHP tab content goes here -->
<div class="row">
<div class="row">
<h2>Overview</h2>
<p>Fish is a source of high protein, vitamins and nutrients for humans. With the population surge, the
<h2 class="reveal-on-scroll">Overview</h2>
<p class="reveal-on-scroll">Fish is a source of high protein, vitamins and nutrients for humans. With the
population surge, the
requirement
for nutrition has skyrocketed. The aquaculture sector plays an important role in the breeding of fish.
India,
being a peninsula, is a major contributor of this sector.</p>
<p>The Department of Fisheries, Ministry of Fisheries, Animal Husbandry and Dairying, are implementing a
<p class="reveal-on-scroll">The Department of Fisheries, Ministry of Fisheries, Animal Husbandry and Dairying,
are implementing a
flagship
scheme <b>“Pradhan Mantri Matsya Sampada Yojana (PMMSY)”</b>—A scheme to bring about Blue Revolution through
sustainable and responsible development of the fisheries sector in India. This scheme has seen the
......@@ -541,7 +582,8 @@
7.56%
of the global production of fish [2].</p>
<p>Since Udupi is a coastal town, this district contributes considerably to the production of fish in India.
<p class="reveal-on-scroll">Since Udupi is a coastal town, this district contributes considerably to the
production of fish in India.
As
a
group of undergraduate students, our insight into this world and people's problems are limited. Therefore to
......@@ -549,7 +591,8 @@
the
problem and find its root cause.</p>
<p>We received our problem statement through a telephone meeting with Mr. Kaushik Alike, the owner of Canares
<p class="reveal-on-scroll">We received our problem statement through a telephone meeting with Mr. Kaushik
Alike, the owner of Canares
Aquaculture LPP. Our interest in this topic grew as we spoke to many more experts, scholars, fish farmers,
etc.,
after which we chose our target bacteria <i>Vibrio parahaemolyticus</i>. Their
......@@ -557,10 +600,10 @@
run.
Our human practices efforts have been the backbone of our project since the beginning. Through our
timeline, we have represented our interactions and their magnanimous impact toward AMPifin.
<p>
<p class="reveal-on-scroll">
</div>
<div class="row">
<h1>Integrated Human Practices Timeline</h1>
<h1 class="reveal-on-scroll">Integrated Human Practices Timeline</h1>
<hr><br>
</div>
<div class="row">
......@@ -568,23 +611,23 @@
<div class="row">
<div class="col">
<span class="ihp-circle" style="background: #D8C200;"></span>
<p>Academia</p>
<p class="reveal-on-scroll">Academia</p>
</div>
<div class="col">
<span class="ihp-circle" style="background: #FF0000;"></span>
<p>Companies</p>
<p class="reveal-on-scroll">Companies</p>
</div>
<div class="col">
<span class="ihp-circle" style="background: #2020F1;"></span>
<p>Outreach</p>
<p class="reveal-on-scroll">Outreach</p>
</div>
<div class="col">
<span class="ihp-circle" style="background: #429F89;"></span>
<p>Doctors</p>
<p class="reveal-on-scroll">Doctors</p>
</div>
<div class="col-3">
<span class="ihp-circle" style="background: #002F40;"></span>
<p>Entrepreneurs</p>
<p class="reveal-on-scroll">Entrepreneurs</p>
</div>
</div>
</div>
......@@ -595,7 +638,7 @@
<br>
<hr>
<p>Click on the tabs below for more details</p>
<p class="reveal-on-scroll">Click on the tabs below for more details</p>
<!-- Person-1 -->
<div class="row">
<!-- Button trigger modal -->
......@@ -609,19 +652,20 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-1Label">Mr. Kaushik Alike</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-1Label">Mr. Kaushik Alike</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<p>Mr. Kaushik Alike, Owner of Canares Aquaculture LPP located in Holanagadde, Kumta, Karnataka,
<p class="reveal-on-scroll">Mr. Kaushik Alike, Owner of Canares Aquaculture LPP located in
Holanagadde, Kumta, Karnataka,
India.</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We contacted Kaushik Alike on 21st November 2021. He is one of the founders
of
Canares Aquaculture. He spoke to us about various topics, including parasites, viruses, feed and
......@@ -629,7 +673,7 @@
us. An important concern he brought up was the increasing threat to the microbial flora of the
water
bodies. He also discussed the several strategies used to distribute feed and raise fish.</p>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
Through this interaction, we started looking into the <i>Vibrio</i> species and discovered their
impact. Furthermore, we observed that the usage of antibiotics was not lost in aquaculture
......@@ -660,7 +704,7 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-2Label">Dr. Ramesh Sistala</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-2Label">Dr. Ramesh Sistala</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
......@@ -669,12 +713,13 @@
<div class="row">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-dr-ramesh-sistala-portrait.jpg">
<p>Dr. Ramesh Sistala, Founder and Managing Director at thinkMolecular Technologies Pvt. Ltd.,
<p class="reveal-on-scroll">Dr. Ramesh Sistala, Founder and Managing Director at thinkMolecular
Technologies Pvt. Ltd.,
Bangalore, India.</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We approached Dr. Ramesh Sistala on 13th April 2022 during the initial stages
of
our project to receive help on the dry lab facet of our project. He gave us some ground rules for
......@@ -685,7 +730,7 @@
the interaction between MAM7 and Fibronectin. Finally, he asked us to make sure that the
hypothesis
we produce is functional by carrying out a trial run.
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-ramesh-sistala-april.jpeg"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Ramesh Sistala (Meeting 1)">
......@@ -694,7 +739,7 @@
</figcaption>
</figure>
<i></i>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
The ideas and suggestions he provided laid the foundation for our Dry Lab work. He was crucial in
helping us find the most appropriate template to work with. We explored the two possibilities he
......@@ -723,20 +768,21 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-3Label">Dr. Nithin M.S.</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-3Label">Dr. Nithin M.S.</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<p>Dr. Nitin Muliya Sankappa, Ph. D. researcher in Fisheries Microbiology, Department of Aquatic
<p class="reveal-on-scroll">Dr. Nitin Muliya Sankappa, Ph. D. researcher in Fisheries
Microbiology, Department of Aquatic
Animal Health Management at College of Fisheries, Karnataka Veterinary Animal and Fisheries
Sciences University, Karnataka, India.</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> Dr. Nithin M.S. is proficient in the field of using bacteriophages to work on
Vibriosis. We approached him on 28th May 2022 to gather data on the prevalence of vibriosis in and
around Manipal. We explained how MAM7 is an adhesion molecule involved in the early stages of the
......@@ -746,14 +792,14 @@
increasing demand for solutions such as AMPifin given the disease's prominence in fish. He also
suggested ways to deliver the peptide, and recommended using bioinformatics tools to test our
solution's efficacy.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-dr-nithin-ms-may.png"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Nithin M S">
<figcaption class="figure-caption text-center">
Interaction with Dr. Nithin M S
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
Via this interaction, we identified the statistical significance of vibriosis and its prevalence.
We
......@@ -780,7 +826,7 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-4Label">Mr. M Madan</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-4Label">Mr. M Madan</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
......@@ -788,13 +834,13 @@
<div class="col-4">
<div class="row">
<img src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-mr-m-madan-portrait.jpeg">
<p>
<p class="reveal-on-scroll">
Mr. M Madan, Feedmill Operator at ICAR-CIBA, Chennai, India.
</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We contacted Mr. Madhan on 29th May 2022 to get data on the basic feeding and
cultivation practices of fish. He told us that vibriosis is quite prevalent in fish breeds such as
Mullet and Milkfish. Being well-versed with
......@@ -809,7 +855,7 @@
elaborated on the various early detection methods such as PCR, western blotting, kit-based
methods,
and microscopic analysis.</p>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
This interaction shed light on the feeding mechanisms practised in the fish farms. The nutritional
value of the fish can only be maintained by providing a balanced supply of the diet. Furthermore,
......@@ -847,14 +893,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-5Label">Dr. Seetha Krishnan</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-5Label">Dr. Seetha Krishnan</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-dr-seetha-krishnan-portrait.jpeg"
class="figure-img img-fluid rounded" alt="Dr. Seetha Krishnan">
......@@ -866,7 +912,7 @@
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We interacted with Dr. Seetha Krishnan on 30th May 2022. She was intrigued by
our
project idea and its potential impact on the pisciculture industry. Based on the stability of our
......@@ -881,7 +927,7 @@
aspects of our project. She recommended we use the HPLC system for regular purification, which
would
be a practical approach for our experiments due to its cost-effectiveness.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-dr-seetha-krishnan-may.png"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Seetha Krishnan">
......@@ -889,7 +935,7 @@
Interaction with Dr. Seetha Krishnan
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
Dr. Seetha laid the foundation for our delivery system. Our interaction with her made us consider
the factors involving the cost-effectiveness of our solution. Through this, we decided
......@@ -921,14 +967,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-6Label">Dr. Mamatha Ballal</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-6Label">Dr. Mamatha Ballal</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-dr-mamatha-ballal-portrait.jpeg"
class="figure-img img-fluid rounded" alt="Dr. Mamatha Ballal">
......@@ -940,7 +986,7 @@
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5>We approached Dr. Mamatha Ballal on 2nd June 2022 to get solutions for our
research-based queries and information on the prevalence of vibriosis. Since vibriosis is a cause
of
......@@ -964,7 +1010,7 @@
mentioned that stool samples are collected to analyse and detect vibriosis in humans. Furthermore,
she elaborated on the culture-based methods for <i>V. parahaemolyticus</i>—i.e., using blood agar
and nutrient agar.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-dr-mamatha-ballal-june.jpg"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Mamatha Ballal">
......@@ -972,7 +1018,7 @@
Interaction with Dr. Mamatha Ballal
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
The interaction with Dr. Ballal helped us gain more statistical data on the reported cases
concerning vibriosis. This helped us build a core understanding of the importance of following
......@@ -1002,14 +1048,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-7Label">Dr. Ramesh Sistala</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-7Label">Dr. Ramesh Sistala</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-dr-ramesh-sistala-portrait.jpg"
class="figure-img img-fluid rounded" alt="Dr. Ramesh Sistala">
......@@ -1021,7 +1067,7 @@
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We had our second interaction with Dr. Ramesh on 28th June 2022 to discuss
the
structure
......@@ -1040,7 +1086,7 @@
residues and changing the others. Furthermore, he elaborated that five or six types of amino acids
are required, and all 20 amino acids need not be required. He further elaborated more on the Build
option in Pymol that could be used for visualising the protein structure.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-dr-ramesh-sistala-2-0-june.png"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Ramesh Sistala">
......@@ -1048,7 +1094,7 @@
Interaction with Dr. Ramesh Sistala
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
In this meeting, Dr. Ramesh provided us with the technique to generate a pool of peptides. This is
where we decided our peptide to act as an inhibitor for our target molecule—MAM7. Furthermore, the
......@@ -1076,14 +1122,15 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-8Label">Dr. Kannan Ramaswamy</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-8Label">Dr. Kannan Ramaswamy</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<p>Dr. Kannan Ramaswamy is a Gastroenterologist at Bhagwan Mahaveer Jain Hospital, Bangalore,
<p class="reveal-on-scroll">Dr. Kannan Ramaswamy is a Gastroenterologist at Bhagwan Mahaveer Jain
Hospital, Bangalore,
and
Sagar Hospitals in Jayanagar, Bangalore. He is a member of the Indian Medical Association
(IMA)
......@@ -1092,7 +1139,7 @@
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We approached Dr. Kannan on 3rd July 2022 to get information on the effect of
vibriosis on human beings. Based on his research, he indicated that vibriosis involves a sporadic
outbreak that is not as prevalent in urban areas since they consume filtered water. With many
......@@ -1109,7 +1156,7 @@
find their way to rural areas, leading to large-scale epidemics that could be lethal beyond a
point.
</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-dr-kannan-ramaswamy-july.jpg"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Kannan Ramaswamy">
......@@ -1117,7 +1164,7 @@
Interaction with Dr. Kannan Ramaswamy
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
The interaction with Dr. Kannan gave us an insight into statistics associated with reported cases
of
......@@ -1148,20 +1195,21 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-9Label">Dr. M Shashi Shekar</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-9Label">Dr. M Shashi Shekar</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<p>Dr. M. Shashi Shekar, Principal Scientist & SIC, Nutrition, Genetics and Biotechnology
<p class="reveal-on-scroll">Dr. M. Shashi Shekar, Principal Scientist & SIC, Nutrition, Genetics
and Biotechnology
Division, working since 1994 in ICAR-CIBA, Chennai, India.
</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5>We visited the Indian Council of Agricultural Research-Central Institute of
Brackishwater Research (ICAR- CIBA) institute located in Chennai on 8th July 2022. Dr. M Shashi
Shekar
......@@ -1173,14 +1221,14 @@
areas
in which they could assist us with. Dr. Shashi Shekar introduced us to his lab and his team which
would help us in the future with our project.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-icar-ciba-july.jpg"
class="figure-img img-fluid rounded" alt="Interaction with Dr. M Shashi Shekar">
<figcaption class="figure-caption text-center">
Interaction with Dr. M Shashi Shekar
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
The visit to their institute assisted us in gathering more information on the field studies of
their
......@@ -1212,19 +1260,19 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-10Label">ICAR-CIBA</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-10Label">ICAR-CIBA</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<p>ICAR-CIBA (Dr. M Shashi Shekar & team), Chennai, India.
<p class="reveal-on-scroll">ICAR-CIBA (Dr. M Shashi Shekar & team), Chennai, India.
</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We approached Dr. Shashi Shekhar and his team from ICAR-CIBA on 10th August
2022
after meeting them at their institute. He introduced us to Dr. Ashok, well-versed in
......@@ -1240,14 +1288,14 @@
range and ammonia concentration that are to be maintained to prevent such outbreaks of diseases in
the fish farm. Furthermore, they appreciated our idea of targeting initial adhesion and discussed
current treatment methods.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-icar-ciba-august.jpg"
class="figure-img img-fluid rounded" alt="Interaction with ICAR-CIBA">
<figcaption class="figure-caption text-center">
Interaction with ICAR-CIBA
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
It was due to Dr. Raymond that we could formulate a design system for our mesh that would fit at
the
......@@ -1275,14 +1323,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-11Label">Dr. Manickam G</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-11Label">Dr. Manickam G</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-dr-manickam-g-portrait.jpeg"
class="figure-img img-fluid rounded"
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</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We interacted with Dr. Manickam on 12th August 2022, to receive inputs on the
economic viability and large-scale production of our peptide. He was impressed with our usage of
chitosan nanoparticles as a delivery system since it is a natural polymer with immunomodulatory
......@@ -1312,7 +1360,7 @@
almost a week. Towards the end, he suggested focusing on the ethical issues associated with the
disposal of the residue, if any, in the environment and also highlighting the impact being visible
on human beings, the end consumers of the food chain.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-dr-manickam-august.jpg"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Manickam G">
......@@ -1320,7 +1368,7 @@
Interaction with Dr. Manickam G
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
The interaction with Dr. Manickam gave us a better understanding of implementation of appropriate
purification methods for commercial production and for the isolation of our peptide using modern
......@@ -1351,14 +1399,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-12Label">Reiss Jones</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-12Label">Reiss Jones</h3>
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<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-mr-reiss-jones-portrait.jpg"
class="figure-img img-fluid rounded" alt="Reiss Jones">
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</figure>
</div>
<div class="row">
<p>Reiss is a sustainability-based entrepreneur and has previously co-founded two startups.
<p class="reveal-on-scroll">Reiss is a sustainability-based entrepreneur and has previously
co-founded two startups.
</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5> We approached Reiss Jones on 16th August 2022 to understand the possible
entrepreneurial aspects we could incorporate in our project. He was quite intrigued with our
team's approach for the peptide design and he mentioned the skyrocketing demand for sustainable
......@@ -1382,7 +1431,7 @@
gist on our plan of performing interaction studies with MAM7 and our peptide. After arriving at a
proper understanding of our project, he helped us by connecting with various synthetic biology
professors and other experts in the field of marine biotechnology.</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-reiss-jones-august.png"
class="figure-img img-fluid rounded" alt="Interaction with Reiss Jones">
......@@ -1391,7 +1440,7 @@
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
He assisted us in navigating the kind of companies we must reach out to and the aquaculture
specialists we can approach for better insight on our project and shared a few helpful
......@@ -1418,14 +1467,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-13Label">Ronald Kevin D’Souza</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-13Label">Ronald Kevin D’Souza</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-mr-ronald-d-souza-portrait.jpg"
class="figure-img img-fluid rounded"
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</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5>We visited Aquatic Biosystems in Mangalore, owned by Mr. Ronald D’Souza on
22nd
August 2022. He set up Aquatic Biosystems in 2006, which is currently the largest ornamental fish
......@@ -1463,7 +1512,7 @@
to balance it out, and in case of any decreased pH range, the oyster shells are utilised as a
buffer.
</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/aquatic-biosystems-outreach-1.jpg"
class="figure-img img-fluid rounded" alt="Interaction with Ronald Kevin D’Souza">
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Interaction with Ronald Kevin D’Souza
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
We were delighted to observe the application of filters in the fish farm. This gave us the
confidence to propose a future implementation of our project in the form of a mesh that the inlet
......@@ -1500,14 +1549,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-14Label">Miroslav Gasparek</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-14Label">Miroslav Gasparek</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-mr-miroslav-gasparek-portrait.jpg"
class="figure-img img-fluid rounded" alt="Mr. Miroslav Gasparek">
......@@ -1517,21 +1566,22 @@
</figure>
</div>
<div class="row">
<p>Mr. Miroslav Gasparek is a Bioengineer, Entrepreneur, and Researcher. He is also a Co-founder
<p class="reveal-on-scroll">Mr. Miroslav Gasparek is a Bioengineer, Entrepreneur, and Researcher.
He is also a Co-founder
at Stealth Biotech Startup and a Co-Managing Director
at Nucleate UK.
</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5>We interacted with Miroslav Gasparek on 26th September 2022, who provided our
team
with insights on the biosafety and biosecurity aspects revolving around our project and synthetic
biology as well. The interaction with him led to have major conversations on how Genetically
Modified Organisms (GMOs) play a crucial role in the current emerging field of synthetic biology
</p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-miroslav-gasparek-september.png"
class="figure-img img-fluid rounded" alt="Interaction with Miroslav Gasparek">
......@@ -1539,7 +1589,7 @@
Interaction with Miroslav Gasparek
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
We were pleasantly surprised by the immediate conceptual grasp he exhibited regarding our project.
We incurred valuable insight on the various aspects of biosafety, good lab practices and
......@@ -1569,14 +1619,14 @@
<div class="modal-dialog modal-fullscreen ihp-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
<h3 class="modal-title" id="ihp-modal-15Label">Dr. Vinod Jyothikumar</h3>
<h3 class="modal-title reveal-on-scroll" id="ihp-modal-15Label">Dr. Vinod Jyothikumar</h3>
<button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-4">
<div class="row">
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-portraits/ihp-dr-vinod-jyothikumar-portait.jpg"
class="figure-img img-fluid rounded" alt="Dr. Vinod Jyothikumar">
......@@ -1586,14 +1636,15 @@
</figure>
</div>
<div class="row">
<p>Dr. Vinod Jyothikumar, currently working as a Operations Management Consultant in a
<p class="reveal-on-scroll">Dr. Vinod Jyothikumar, currently working as a Operations Management
Consultant in a
consulting
firm.
</p>
</div>
</div>
<div class="col-8">
<p>
<p class="reveal-on-scroll">
<h5>THEIR INPUT</h5>We interacted with Dr. Vinod Jyothikumar on 28th September 2022 to receive
inputs on
the biosafety aspects associated with our project and how we have integrated synthetic biology
......@@ -1606,7 +1657,8 @@
express Multivalent Adhesion Molecule 7 (MAM7) in <i>Escherichia coli</i>. He highlighted the
biochemistry and biophysics aspects involved in making our peptide more commercially realistic
</p>
<p>He spoke about how we could include a visual signal for our binding by making use of a GFP. He
<p class="reveal-on-scroll">He spoke about how we could include a visual signal for our binding by
making use of a GFP. He
asked us about the parameters that affect the pathogenicity of <i>Vibrio parahaemolyticus</i>,
such that we could derive an ideal condition that renders this bacteria harmless. For the
His-tag
......@@ -1616,7 +1668,7 @@
urged
us to include a visual proof of the MAM7 and peptide interaction. The major application of
visualising, would involve methods of integration of quantum dots. </p>
<figure class="figure">
<figure class="figure reveal-on-scroll">
<img
src="https://static.igem.wiki/teams/4200/wiki/ihp-timeline-pictures/ihp-dr-vinod-jyothikumar-september.png"
class="figure-img img-fluid rounded" alt="Interaction with Dr. Vinod Jyothikumar">
......@@ -1624,7 +1676,7 @@
Interaction with Dr. Vinod Jyothikumar
</figcaption>
</figure>
<p>
<p class="reveal-on-scroll">
<h5>OUR INFERENCE</h5>
The interaction with Dr. Vinod helped us look for a more realistic approach of synthetic-biology
to
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