add work for experiments

wiki/pages/experiments.html

@@ -64,13 +64,38 @@
          <li>Additionally, 2 isolated plasmids from each construct is sequenced at joint regions by the Sanger method. </li>
          <li>Finally, whole plasmid sequencing by Next Generation Sequencing (NGS) on two plasmids–one each from randomly selected plasmid strain.</li>
       </ol>
-      
+
      <h6><b>Notes</b></h6>
       <p>The gene sequences (AiOSC1, AiCYP71D2 and candidate Melia ) were extracted from the NCBI database 
         from a study that obtained the sequences using cDNA obtained from RNA extracted from
          the A.indica plant (Hou et al). The gene fragments were synthesized from Twist Bioscience.
       </p>
-    
+
+      <h4>SOP #2 Yeast strain construction</h4>
+      <hr>
+      <p>For constructing an efficient dihydroniloticin-producing strain, two strains are to 
+        be made, one control strain with the AiOSC1 (2,3 oxidosqualene cylase) and AiCYP71D2 
+        (azadirachta indica cytochrome oxidase) fragment containing plasmid and the AiOSC1 
+        and candidate Melia azederach cytochrome oxidase fragment containing plasmid.
+      </p>
+      <h6><b>Materials</b></h6>
+      <ul>
+        <li>Constructed plasmids</li>
+        <li>Water bath</li>
+        <li>CSM-Ura agar plates</li>
+        <li>Incubator</li>
+        <li>Sterile water</li>
+     <li>Shaking incubator</li>
+     <li>Centrifuge</li>
+     <li>Microcentrifuge</li>
+     <li>Falcon tubes</li>
+     <li>Vortexer</li>
+     <li>Micropipette and pipette tips</li>
+     <li>Polyethylene glycol</li>
+     <li>Lithium acetate</li>
+     <li>Microcentrifuge tubes</li>
+     <li>Spreader</li>
+      </ul>
   </div>
   <div class="col-lg-4">
     <h2>Inspirations</h2>