We have managed to create a library of 5'UTRs that gives a range of expression. The key learning was on how just 9 nucleotides were enough to influence the gene expression as observed in the case of cspF. We are still trying to interpret the complex structures of the naturally occurring UTRs. Our next experimental design shall be more complex based on our understandings and observations of GC content. We further plan to add an additional factor of complexity to our next set of experiments by looking at RNAse cleavage sites. We have realised that experimental results are quite different from software predictions this further justifies the need for more work and attention in the area of 5'UTR. We are completing the learn phase of the second engineering cycle and are contemplating simultaneously over the design of the third as described on our engineering success page. We further wish to develop correlations based on experimentally validated data and build on our 5'UTR Library.</p>
