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Commit 8b4a7bd2 authored by Arya Ajit Gohad's avatar Arya Ajit Gohad
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Update wiki/pages/engineering-test.html

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......@@ -24,7 +24,7 @@ The results of our first 8 UTR constructs served as a basis for the dry-lab work
Now that we had laboratory-established data for 19 UTR constructs with reporter genes RFP, we got curious about the effect of the same UTRs with other gene sequences. So, we decided to use amilCP to clear our curiosity. We decided to insert 12 of the existing UTR sequences and repeated the protocol to obtain recombinant vectors. However, to our disappointment, we figured it all had been in vain. During our analytical procedure, we realized that the fluorescence and optical density ratio was always 1. This was because the Lambda max of amilCP is 588 nm, and optical density is read at 600 nm. This leads to a significant overlap of the peaks. All our test experiments were performed in duplicates.
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<p><h2>Test Results for DH5α Strain:</h2><br>
<p><center><h2>Test Results for DH5α Strain:</h2></center><br>
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<center><h3>For first 8 UTRs of DH5α:</h3><br></centre><center>
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