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Edinburgh-UHAS_Ghana
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2022 Competition
Edinburgh-UHAS_Ghana
Commits
5c0897c6
Commit
5c0897c6
authored
2 years ago
by
Maarten van den Ancker
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Replace experiments.html
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@@ -86,7 +86,7 @@
<h3>
Transformations
</h3>
<ol>
<li>
10 µl of plasmid DNA or reaction mixture were added to 100 µl of competent E. coli cells and left on ice for 30 minutes
</li>
<li>
10 µl of plasmid DNA or reaction mixture were added to 100 µl of competent
<i>
E. coli
</i>
cells and left on ice for 30 minutes
</li>
<li>
The cells were heat shocked at 42 °C for 30 seconds, then returned to ice for 30 seconds.
</li>
<li>
1 ml of SOC media was added.
</li>
<li>
The cells were incubated at 37 °C and 200 rpm for 45 minutes.
</li>
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