<li>20 ml of the stock solution were transferred into a new container and 3 g of citric acid were added while stirring to make a 15% w/v citric acid/CMC solution. </li>
<li>The solution was poured into casts and incubated at 40 °C for 24 hours to remove water. </li>
<li>After dehydration, the temperature was increased to 80 °C and the mixture was incubated for a further 24 hours to facilitate crosslinking. </li>
<li>The hydrogel was washed and dehydrated at 40°C for 24 hours.</li>
<li>The hydrogel was dehydrated at 40°C for 24 hours for storage.</li>
</ol>
<h3>CBD Fusion Protein CMC-Binding Assay</h3>
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@@ -597,7 +597,7 @@
<h3>ICP-MS Sample preparation</h3>
<ol>
<li>The CBD-MT and lacZ lysates were thawed out on ice and diluted with 7.5 pH Tris-HCl to a protein concentration of 1 g/ml. </li>
<li>The CBD-MT and lacZ lysates were thawed out on ice and diluted with 7.5 pH Tris-HCl to a protein concentration of 1 mg/ml. </li>
<li>1 ml of a diluted lysate was added to each hydrogel as shown in the following table.
