Nowadays, the occurrence of CKD is universal, and according to one of the top medical journals THE LANCET, it says
that by 2017, the number of CKD patients in the world had reached 697.5 million, among which there are 132.3
million
of Chinese patients with 9.5% incidence rate of CKD. In other words, on average, one out of every 10 Chinese is
suffering from CKD. What's worse, CKD is pretty detrimental to human bodies, such as significantly increasing the
incidence rate of getting heart and cerebrovascular disease. Based on such a serious condition, we are motivated
to
find a way to cure this disease.
N-butanol, an organic compound(C4H10O), is a colorless transparent liquid. There is a larger demand for N-butanol than before. N-butanol can take advantage of its organic compounds to help transport hydrogen and reduce the waste of electricity, while also being a less polluting fuel. However, there are many disadvantages in the traditional N-butanol manufacturing process. Thus, our purpose is to produce N-butanol more efficiently by biosynthesis as well as be more friendly to the environment. Below is the method, Streptomyces acetobutyrate genes thl, hbd, crt and streptomyces dentatus ter need to be cloned into a plasmid expressing butanol synthetic pathway proteins. The plasmid carrying these genes with optimized codon will be introduced into the target strain through electrical transformation to test whether the transformants can successfully synthesize N-butanol. After that, the expression of the strain will be further optimized to successfully construct lactobacillus that could produce N-butanol using glucose as raw material.
