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Commit 2aa6ebe7 authored by Yangyang Kong's avatar Yangyang Kong
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Update wiki/pages/engineering.html

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<div class="article-content">To build the plasmid, firstly amplified the thlA, crt, hbd, and ter genes
fragments from the Lactobacillus Brevis ATCC824 genomic DNA (Figure 2). The second step was to obtain the linear
carrier. The third step was to ligate the genes and linearized vector and transfer the ligation product into E.
coli DH5α competent. To build the plasmid, firstly amplified the thlA, crt, hbd, and ter genes fragments from the
Lactobacillus Brevis ATCC824 genomic DNA (Figure 2). The second step was to obtain the linear carrier. The third
step was to ligate the genes and linearized vector and transfer the ligation product into E. coli DH5α competent.
coli DH5α competent.
</div>
<div class="img-wrap no-margin">
<img class="w-40" src="https://static.igem.wiki/teams/4515/wiki/t-east-china-engineering02.png" />
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