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2022 Competition
East_China
Commits
2aa6ebe7
Commit
2aa6ebe7
authored
2 years ago
by
Yangyang Kong
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Update wiki/pages/engineering.html
parent
ac62e362
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main
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2 years ago
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2aa6ebe7
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@@ -39,9 +39,7 @@
<div
class=
"article-content"
>
To build the plasmid, firstly amplified the thlA, crt, hbd, and ter genes
fragments from the Lactobacillus Brevis ATCC824 genomic DNA (Figure 2). The second step was to obtain the linear
carrier. The third step was to ligate the genes and linearized vector and transfer the ligation product into E.
coli DH5α competent. To build the plasmid, firstly amplified the thlA, crt, hbd, and ter genes fragments from the
Lactobacillus Brevis ATCC824 genomic DNA (Figure 2). The second step was to obtain the linear carrier. The third
step was to ligate the genes and linearized vector and transfer the ligation product into E. coli DH5α competent.
coli DH5α competent.
</div>
<div
class=
"img-wrap no-margin"
>
<img
class=
"w-40"
src=
"https://static.igem.wiki/teams/4515/wiki/t-east-china-engineering02.png"
/>
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