The <i>Staphylococcus aureus</i>(<i>S.aureus</i>) contamination is a common problem that we encounter everyday. To address this , our project is designed to utilize protein proximity labeling technology in the concept of real life to inhibit the growth of <i>S.aureus</i>, therefore preserving the food from bacterial contamination. The scientific theory behind our project is that we reduce the growth of <i>S.aureus</i> by blocking down the QS channels in the process of protein biotinylation, which fused protein to bring TurboID to the specific QS site AgrB and AgrC. In order to obtain more biotin and keep the reaction going on, we use engineered Escherichia coli to produce it. Therefore, with enough TurboID-AIP and biotin, the growth of <i>S.aureus</i> will be blocked and our health can be preserved in this way. We identified three potential situations that need our product and the specific treatments are exerted on diverse conditions.
The <i>Staphylococcus aureus</i>(<i>S.aureus</i>) contamination is a common problem that we encounter everyday. To address this , our project is designed to utilize protein proximity labeling technology in the concept of real life to inhibit the growth of <i>S.aureus</i>, therefore preserving the food from bacterial contamination. The scientific theory behind our project is that we reduce the growth of <i>S.aureus</i> by blocking down the QS channels in the process of protein biotinylation, which fused protein to bring TurboID to the specific QS site AgrB and AgrC. In order to obtain more biotin and keep the reaction going on, we use engineered Escherichia coli to produce it. Therefore, with enough TurboID-AIP and biotin, the growth of <i>S.aureus</i> will be blocked and our health can be preserved in this way. We identified three potential situations that need our product and the specific treatments are exerted on diverse conditions.
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<h2>Identification of potential users</h2>
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@@ -40,7 +40,7 @@ The <i>Staphylococcus aureus</i>(<i>S. aureus</i>) contamination is a common pro
<b>Household preservation</b><br><br>
We design this type of preservative film with the extracting solution of proteins on it, which can prevent the food from undergoing biofilm effect and the accumulating of bacteria.<br><br>
<b>Restaurants kitchens’ hygiene</b><br><br>
Our special preservative film will prevent food from absorbing <i>S.aureus</i> on it.<br><br>
Our special preservative film will prevent food from absorbing <i>S.aureus</i> on it.<br><br>
<b>Fresh milk transformation</b><br><br>
We use silver paper to seal the used glass milk bottles. Therefore, with less bacteria growth inside the bottle, it can be recycled hygienically.<br>
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@@ -63,7 +63,7 @@ Fig.2 The use of our product: split protein extracting solution on the film
Fig.3 The protein-treated film can be applied to inhibit the growth of S.aureus
Fig.3 The protein-treated film can be applied to inhibit the growth of <i>S.aureus</i>
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@@ -86,15 +86,15 @@ Moreover, the cost in this process is also taken into major consideration. In th
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<h2>Protocals of testing S.aureus food contamination</h2>
<h2>Protocals of testing <i>S.aureus</i> food contamination</h2>
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Here, we invent a new way to detect the S.aureus by using the filter bottle. We add the food/liquid to the upper bottle and culture with TurboID-AIP, Biotin and ATP. When we vacuum the liquid in the upper bottle, the bacteria can not get through the 0.2 um filter member. So we incubate the member with streptavidin-HRP for 30 min and followed by washing with PBS. The HRP can react with the SuperSignal™ West Pico PLUS Chemiluminescent Substrate. The membrane can be taken out and imaged to show up the blot on the membrane. Thus, the dark blot on the membrane is the S.aureus.
Here, we invent a new way to detect the <i>S.aureus</i> by using the filter bottle. We add the food/liquid to the upper bottle and culture with TurboID-AIP, Biotin and ATP. When we vacuum the liquid in the upper bottle, the bacteria can not get through the 0.2 um filter member. So we incubate the member with streptavidin-HRP for 30 min and followed by washing with PBS. The HRP can react with the SuperSignal™ West Pico PLUS Chemiluminescent Substrate. The membrane can be taken out and imaged to show up the blot on the membrane. Thus, the dark blot on the membrane is the <i>S.aureus</i>.
