@@ -79,6 +79,15 @@ When pbr R reacts for a period of time, the remaining binding amount of pbrR can
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During plasmid construction, our team and Jilin_China jointly optimized the vector selection and expression system,and continue to verify in the future.
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Our purpose is to detect the quantity of the monomer pbrR protein which can combine with the turboid-pbrR fusion protein.And with the combination of the biotin and streptavdin-cy3, the fluorescent signal can be observed.<br><br>
With different concentration of the Pb<sub>2+</sub> in the culture medium:(A)0mM;(B)1μM;(C)10μM;(D) 1mM.DAPI channel was used the concentration of the bacteria,the Cy3 channel was used to verify the quantity of the pbrR protein which is not combined with the Pb<sub>2+</sub>.The result showed that the number of the monomer pbrR protein decreased when there is a higher concentration of the Pb<sub>2+</sub>.This provided us with the possibility to evaluate the function of our surface display system.
