Here, we invent a new method to block the quorum sensing system in <i>S. aureus</i>. At level 0 which is the rest state, autoinducer protein (AIP) can interact with AgrC, followed by downstream activation of AgrA. AgrA is a quorum-sensing system that levels up the contents of many poisons and virulence determinants when the density of cells raise to a certain amount. AgrA can also amplify the quorum sensing signaling by initiating the expression of downstream gene cluster named agrBCDA. The AgrD protein can interacted with AgrB, then AgrD will be spliced into three parts and AIP can release into the environment to enhance quorum sensing signaling. At level 1, we purified the protein named TurboID-N-AgrD-AIP and these protein can have high affinity on interacting with AgrA through AIP domain and also have high affinity on interacting with AgrD through N-AgrD domain. At level 2, the chemical reaction (other named Protein proximity labeling) happens when we apply the system with biotin and ATP. TurboID will catalyze the biotin and ATP with the lysine residue on the surface of AgrA and AgrB. Those channel proteins have a lot of lysine exposed and will be prevented by interaction with AIP. As such, we can use this specific way to block the the quorum sensing system in <i>S. aureus</i>.
