<figcaptionclass ="fig-cap">Figure 9: Flow rate trials vs. final droplet size</figcaption>
</figure>
<p>The next step in the process is incubation. Here we measured the incubator’s temperature while the incubator was heating up until after the temperature had stabilized. The goal for this step was for the incubator to heat up and stabilize in about 30 minutes in order to optimize the preparation time before the droplets are generated. Overall, this test showed the heat up and stabilization time is around 30 minutes, and the incubator will keep the set temperature for the entire incubation period. This is important for the stimuli to induce the biosensor’s production of the desired output at a detectable level by the second microfluidic device.</p>
<p>The next step in the process is incubation. Here we measured the incubator’s temperature from room temperature until after the temperature had stabilized. The goal for this step was for the incubator to heat up and stabilize in about 30 minutes in order to optimize the preparation time before the droplets are generated. Overall, this test showed the heat up and stabilization time is around 30 minutes, and the incubator will keep the set temperature for the entire incubation period. This is important for the stimuli to induce the biosensor’s production of the desired output at a detectable level by the second microfluidic device.</p>
<sectionid="Micro2"></section>
<h2>Microfluidic Device II: Spacing and Sensing</h2>