@@ -111,7 +111,7 @@ After redoing a golden gate assembly to fix the problem, we did a kinetics assay
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<p>The result (shown in fig.1) shows that the minimal concentration of crystal violet required to get observable protein expression is 0.02µM, and the expression level reaches a maximum when crystal violet concentration reaches 0.2µM. For higher concentrations of crystal violet, the expression level per strain decreases by about 25% while the exponential phase of strain growth delays, probably due to the burden of expression and the slight cytotoxicity of crystal violet. Overall, the optimal concentration of crystal violet for induction is 0.2µM. </p><br><br>
<p>The result (shown in figure 2.) shows that the minimal concentration of crystal violet required to get observable protein expression is 0.02µM, and the expression level reaches a maximum when crystal violet concentration reaches 0.2µM. For higher concentrations of crystal violet, the expression level per strain decreases by about 25% while the exponential phase of strain growth delays, probably due to the burden of expression and the slight cytotoxicity of crystal violet. Overall, the optimal concentration of crystal violet for induction is 0.2µM. </p><br><br>
