<small><em>Table 1.relative fluorescent intensity of HOLMES reaction system with different templates. </em></small>
<small><em>Graph 2.relative fluorescent intensity of HOLMES reaction system with different templates. </em></small>
</p>
<p>The failure in HOLMES might have several causes. Due to the limitation of apparatus and reagants, we can’t do an RNA electrophoresis to verify the length and purity of our sgRNA. Also, the Cas12b protein’s concentration and purity might not be high enough. (the Cas12b protein used in this experiment is without the ProQC system)</p>
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@@ -162,7 +162,7 @@ After redoing a golden gate assembly to fix the problem, we did a kinetics assay