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Commit 3540891b authored by Yuan Xia's avatar Yuan Xia
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Update wiki/pages/contribution.html

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<p>Previous studies have shown the feasibility of using green fluorescent protein (GFP) as a quantifiable reporter gene, so it was available to use the intensity of GFP fluorescence to reflect the amount of protein expression upstream. The GFP intensity over OD value can show the amount of protein expression. The ratio due to the different concentration of arabinose is visualized in Figure 5.</p>
<p style="text-align:center">
<img src="https://static.igem.wiki/teams/4204/wiki/contribution-6-new.png" style="width:70%">
<img src="https://static.igem.wiki/teams/4204/wiki/ara-induction-curve-2.png" style="width:70%">
</p>
<small style="text-align:center;" ><em>Figure 5. The arabinose titration curve</em></small>
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<h4><strong><em>Result</em></strong></h4>
<P>By finding the steady state of strains in each group of concentration, we build a standard curve (fig.1) of different concentrations of RHA induction. With higher rhamnose concentration, the Abs 600 of the bacteria culture will be higher when reaching a steady state. The critical point of rhamnose's concentration is 10mM. At 10 mM, the GFP fluorescence/Abs 600 in the steady state reaches the maxim value; after 10mM, there is no significant growth in GFP fluorescence/Abs 600 as the concentration of Rha increases.</p>
<p style="text-align:center">
<img src="https://static.igem.wiki/teams/4204/wiki/rha-induction-curve.png" width="70%">
<img src="https://static.igem.wiki/teams/4204/wiki/rha-induction-curve-2.png" width="70%">
<br><br>
<small><em>Figure 7. The value of fluorescent level/Abs600 in different concentrations of rhamnose in their steady state.</em></small>
</p>
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