<p>On June 26th, 2022, we joined the online meetup held by iBowu-China to share our project overview with other teams. Our project’s innovation of using nucleic acid detection approaches to detect B. cereus gained praise from students and teachers. While inspiring others with our project design, we also received some profound questions during the meetup. For instance, we planned to use Holmes to detect the target DNA in the genome of B. cereus after lysis in our project. It is because Holmes can detect trace amounts of DNA in solution with high efficiency and rapid detection. A teacher in the meetup asked whether we considered another efficient nucleic acid detection system. Although we did not find a more suitable system at that time, we found it afterward. </p>
<p>On June 26th, 2022, we joined the online meetup held by iBowu-China to share our project overview with other teams. Our project’s innovation of using nucleic acid detection approaches to detect <em>B. cereus</em> gained praise from students and teachers. While inspiring others with our project design, we also received some profound questions during the meetup. For instance, we planned to use Holmes to detect the target DNA in the genome of <em>B. cereus</em> after lysis in our project. It is because Holmes can detect trace amounts of DNA in solution with high efficiency and rapid detection. A teacher in the meetup asked whether we considered another efficient nucleic acid detection system. Although we did not find a more suitable system at that time, we found it afterward. </p>
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<p> In September, we found a just discovered enzyme called the LwaCas13a variant which enhanced collateral activity by engineering LwaCas13a and can be used for ultrasensitive detection of nucleic acids. The advantage of this LwaCas13a variant system over Holmes is that it does not rely on nucleic acid extraction (Yang et al., 2022). It allows for more sensitive and rapid detection of emergent pathogens. By using this detection system in the future, the accuracy and efficiency of our detection of B. cereus may be further improved by using this detection system.
<p> In September, we found a just discovered enzyme called the LwaCas13a variant which enhanced collateral activity by engineering LwaCas13a and can be used for ultrasensitive detection of nucleic acids. The advantage of this LwaCas13a variant system over Holmes is that it does not rely on nucleic acid extraction (Yang et al., 2022). It allows for more sensitive and rapid detection of emergent pathogens. By using this detection system in the future, the accuracy and efficiency of our detection of <em>B. cereus</em> may be further improved by using this detection system.
