<div>1. The structure of anchor protein and passenger protein is easy to affect each other, interferes with their correct folding, and reduces the activity of passenger protein </div>
<div>2. Different transporter proteins will affect the physiological function of the recipient bacteria (growth instability, etc.)</div>
<div>After filtering these required properties, we obtained several satisfied anchor proteins: </div>
<div>其他可用的蛋白</div>
<div>Lipoprotein, S-layer protein and INP.</div>
<div>From them, also followed the instructions given by our advisors, we eventually decided to use ice-nucleation protein, since its advantages in cell surface display technology:</div>
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<divclass="title2"style="font-weight: bold;">-Pros of INP</div>
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  We accomplished this section together, not only for better effectiveness but also to allow our three teams to bring out our diverse strengths, to make a greater performance. Moreover, more audiences are involved because we separately propagandize the lectures. These advantages can be only shown between team collaboration, therefore we are able to achieve a better job.
<div>2. 2. Zhang, Z. et al. (2016) Surface immobilization of human arginase-1 with an engineered ice nucleation protein display system in E. coli, PloS one. Public Library of Science. </div>
