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Commit 8688cce9 authored by Hengran Chu's avatar Hengran Chu
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Update wiki/pages/attributions.html, wiki/pages/collaborations.html,... 

Update wiki/pages/attributions.html, wiki/pages/collaborations.html, wiki/pages/communication.html, wiki/pages/contribution.html, wiki/pages/description.html, wiki/pages/engineering.html, wiki/pages/human-practices.html, wiki/pages/implementation.html, wiki/pages/inclusivity.html, wiki/pages/index.html, wiki/pages/model.html, wiki/pages/notebook.html, wiki/pages/partnership.html, wiki/pages/proof-of-concept.html, wiki/pages/safety.html, wiki/pages/sustainable.html, wiki/pages/team.html
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wiki/pages/attributions.html
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<span>info@hsdf.com</span>
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<div style="background-color: #676b6e;height: 1px;width: 90%;margin: 2rem 0 1rem 5%;"></div>
<div style="background-color: #676b6e;height: 1px;width: 68%;margin: 2rem 0 1rem 16%;"></div>
<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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wiki/pages/collaborations.html
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&emsp;&emsp;At the beginning of the experiment, team LZU-HS-Pro-A helped us prepare consumables, such as ddH2O, 0.1M CaCl2, and a liquid culture medium. Since our experiments are similar in several parts, we can save time by reducing repetitive procedures. Similarly, they had our plasmid extraction done, so we did not have to spend extra time there. During the experiment, the problem they faced is the lack of materials. They had an inefficient AMP to make AMP antibiotic mother solution, and they were stuck due to this limitation. At that time, our team gave them the excess AMP that we own to help them continue. We try our best to solve all the issues we met and try to experiment as effectively as possible.
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<div class="text_block">
<span id="part2" style="font-size: 3rem;">Assistance during experiment with LZU-HS-China-A</span>
<div class="title2">
&emsp;&emsp;We conducted the experiment in the same laboratory with LZU-HS-China-A as well. Firstly, we reminded each other about safety during the experiment. When using the clean bench, we needed to spray alcohol on the item that has to enter. Plus, when conducting electrophoresis, we must wear second gloves, to protect us from harmful chemicals. Then, when balancing the centrifuge, we checked each other’s balance again for safety concerns. Lastly, we shared the conference room, and we communicated the experience that helped us more familiar with the experimental apparatus, and also raised our awareness of safety.
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<span id="part3" style="font-size: 3rem;">Articles revise and improvement</span>
<div class="title2">
&emsp;&emsp;Since English is a second language for us, we need to edit and correct our articles carefully to reduce the number of grammatical mistakes. Therefore, our team collaborated with team LZU-HS-China-A, to double-check and fix the language problems. We swapped our articles, and after reading meticulously, we pointed out all the mistakes we made, and tried our best to amend them. After this collaboration, we believe the quality of our content has been hugely improved.
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<span id="part4" style="font-size: 3rem;">Research and selection based on anchor protein used in cell surface display</span>
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&emsp;&emsp;In the end, by experiments, the efficacious result proves that our discussion and collaboration are successful and vital. During this experience, collaboration allowed us to work more effectively by urging and encouraging each other. Also, we were able to interact with our ideas and brainstorm together, which is particularly beneficial for our projects’ promotion.
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<span id="part5" style="font-size: 3rem;">Projects popularization</span>
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<span>info@hsdf.com</span>
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<div style="background-color: #676b6e;height: 1px;width: 90%;margin: 2rem 0 1rem 5%;"></div>
<div style="background-color: #676b6e;height: 1px;width: 68%;margin: 2rem 0 1rem 16%;"></div>
<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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wiki/pages/communication.html
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&emsp;&emsp;To let more people of different ages and various fields know about synthetic biology, our team holds several activities. In these activities, we not only introduce the knowledge about synthetic biology, but also the contents of our team project and IGEM. We hope that these efforts we have made can improve public awareness of synthetic biology.
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<span id="part2" style="font-size: 3rem;">WeChat official account</span>
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&emsp;&emsp;Our parents, classmates, friends and teachers followed our WeChat official account, even some strangers. Our articles are simplified but very easy to understand and include all the main ideas. So people are able to learn about synthetic biology in their spare time. These articles not only let more people around us know more about synthetic biology field, and also let them to be interested with that. We have wider audience to read our articles. In another word, more and more people know about that by our WeChat official account.
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<span id="part3" style="font-size: 3rem;">Lecture in BFSU International Curriculum Centre</span>
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<img style="width: 40%;" src="https://static.igem.wiki/teams/4400/wiki/education/pic6.jpg" alt="">
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<span id="part5" style="font-size: 3rem;">Education materials</span>
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<img style="width: 40%;" src="https://static.igem.wiki/teams/4400/wiki/education/pic14.png" alt="">
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<span id="part4" style="font-size: 3rem;">Questionnaire</span>
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<span>info@hsdf.com</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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wiki/pages/contribution.html
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<div>&emsp;&emsp;Further information about this can be seen in our project description --- link.</div>
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<span id="part2" style="font-size: 3rem;">Advices</span>
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<span id="part3" style="font-size: 3rem;">References list</span>
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<span>info@hsdf.com</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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wiki/pages/description.html
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<img src='https://static.igem.wiki/teams/4400/wiki/description/pic1.png' alt=''>
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&emsp;&emsp;The recombinant strain was constructed to contain the plasmid psb1a3-Tyr, which encodes the fusion protein InaK-Tyr.
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<span>info@hsdf.com</span>
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<div style="background-color: #676b6e;height: 1px;width: 90%;margin: 2rem 0 1rem 5%;"></div>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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wiki/pages/engineering.html
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<div>&emsp;&emsp;The detection reaction is not obvious, because these chemical molecules need to be transported across the membrane, which may hinder the efficiency of the reaction. When the concentration of BPA is low, the corresponding concentration is not well detected. Consider using surface display technology to increase the chances of BPA molecules coming into contact with the bio detector, thereby increasing sensitivity.</div>
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<span id="part2" style="font-size: 3rem;">Second Round</span>
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<span>info@hsdf.com</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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wiki/pages/human-practices.html
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<span>info@hsdf.com</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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wiki/pages/implementation.html
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&emsp;&emsp;Environmental pollution has increased owing to rapid population growth and the consequent industrialization, which has become one of the major concerns for human health and socioeconomic issues. One of the most significant environmental pollution --Bisphenol A, which poses a threat to wildlife and human health even at low doses. The monitoring of environmental contaminants is crucial for controlling pollution. Traditional physicochemical detection methods focus on high-performance technologies such as ultraviolet spectrometry and liquid chromatography. These approaches provide accurate analysis of environmental samples and have high sensitivity. However, they require costly analytical apparatus and are time-consuming, which makes them unsuitable for in-situ and rapid analysis. Thus, it is desirable to develop a simple and practical analytical method for the detection of environmental pollutants. So the biosensor we designed largely helped solve all these problems, which has the characteristics that is rapid, sensitive, inexpensive, and suitable for on-site monitoring.
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<span id="part2" style="font-size: 3rem;">Target user of bisphenol A biosenor</span>
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<div>4. Diagnose the result </div>
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<span id="part3" style="font-size: 3rem;">Usage of our device </span>
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<div>Voltage 100mV, ph7.0, temperature 37℃ (optimum conditions for tyr)</div>
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<span id="part4" style="font-size: 3rem;">Reaction Principle</span>
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&emsp;&emsp;An electrochemical biosensor was developed for bisphenol A detection by displaying tyrosinase on the surface of Escherichia coli cells, followed by adsorption onto a glassy-carbon electrode. Tyrosinase oxidizes BPA to form Catehol, and then oxidizes Catehol to form O-Quinone, which can release electrical energy and convert BPA concentration by detecting current.
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<span id="part5" style="font-size: 3rem;">Risk Assessment</span>
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<span>info@hsdf.com</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<span>info@hsdf.com</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<span>info@hsdf.com</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<div class="title1">Week 2 (Mon Apr 35 22 - Sun May 1 22)</div>
<div class="title2">&emsp;&emsp;We looked at the website ourselves, including examplary projects, namely engineering, human practice, and wiki. </div>
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<span id="part2" style="font-size: 3rem;">May</span>
<div class="title1">Week 3 (Mon May 2 22- Sun May 8 22)</div>
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<div class="title2">We determined the content of the project and began to plan the experiment.</div>
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<span id="part3" style="font-size: 3rem;">June</span>
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<img src="https://static.igem.wiki/teams/4400/wiki/notebook/pic10.jpg" alt="">
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<span id="part4" style="font-size: 3rem;">July</span>
<div class="title1">Week 11 (Mon Jul 4 22 - Sun Jul 10 22)</div>
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<img src="https://static.igem.wiki/teams/4400/wiki/notebook/pic14.jpg" alt="">
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<span id="part5" style="font-size: 3rem;">August</span>
<div class="title1">Week 15 (Mon Aug 1 22 - Sun Aug 7 22) </div>
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<span>info@hsdf.com</span>
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<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<div>&emsp;&emsp;LZU-HS-China-A found that a large amount of shrimp and crab shell waste is generated every year, and the team believes that this waste can better play its role. LZU-HS-China-A attempts to convert chitosan into chitosan oligosaccharides by cell surface display.</div>
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<span id="part2" style="font-size: 3rem;">Research and selection based on anchor protein used in cell surface display</span>
<div class="title2">&emsp;&emsp;Firstly, and most importantly, since we both use cell surface display technology in our projects, we can help each other technically. In the earlier phase, the problem we faced was choosing a proper anchor protein, which is an essential protein used in cell surface display technology. This is a critical section of the projects for our three teams, but we have no idea which kind of protein can be used, so during our first online meeting, we set up a detailed plan for our projects’ future progress.</div>
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<div class="title2">&emsp;&emsp;In the end, by experiments, the efficacious result proves that our discussion and collaboration are successful and vital. During this experience, collaboration allowed us to work more effectively by urging and encouraging each other. Also, we were able to interact with our ideas and brainstorm together, which is particularly beneficial for our projects’ promotion. </div>
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<span id="part3" style="font-size: 3rem;">Projects popularization</span>
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<span id="part4" style="font-size: 3rem;">Information collection and preparation for synthetic biology education</span>
<div class="title2">&emsp;&emsp;After some time, when preparing for the section on education, we cooperated again for better performance on what we can contribute to the public and synthetic biology. We firstly divided the workload via online meetings, in which our team was in charge of presenting, LZU-HS-China-A for slides and poster creation, also online lecture, and LZU-HS-China-C for related content preparation. This work division was based on our advantages of owning a proper venue for education, outstanding design skills from LZU-HS-China-A, and LZU-HS-China-C who are good at information collecting.</div>
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<div class="title2">&emsp;&emsp;We accomplished this section together, not only for better effectiveness but also to allow our three teams to bring out our diverse strengths, to make a greater performance. Moreover, more audiences are involved because we separately propagandize the lectures. These advantages can be only shown between team collaboration, therefore we are able to achieve a better job.</div>
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<span id="part5" style="font-size: 3rem;">Conclusion</span>
<div class="title2">&emsp;&emsp;Our three teams mainly collaborated in three aspects throughout the season, consisting of selecting INP, project popularization, and education. For us, the partnership is much more than “for a less workload”, or “best showing our strengths”. Without doubts, these are prominent traits of cooperation, besides, our cooperation and communication skills have been significantly enhanced. However, after facing uncountable difficulties as trustworthy allies, and also as high school students, our abilities to get over hardships have incredibly improved. In addition, most importantly, we are very honored to encounter such more friends who are also fond of the field of synthetic biology, and we are willing to contribute more to synthetic biology in the future.</div>
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<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<span id="part2" style="font-size: 3rem;">Biosafety for specific projects</span>
<div class="title2">&emsp;&emsp;Our experiment need to perform experimental operation in the ultra-clean table, also had the use of the toxic substance ethidium bromide in nucleic acid electrophoresis. We require team members: to spray alcohol with both hands to disinfect it when operating in the ultra-clean table, then put it into the ultra-clean table to dry the alcohol before the next operation; cultivate Escherichia coli When you don't touch the lower part of the test tube plug, put the test tube plug on the alcohol lamp for disinfection before and after use, and use the alcohol lamp when opening the LB medium bottle cap; when performing nucleic acid electrophoresis, you should wear double gloves and the outer layer is plastic gloves to avoid contact with ethidium bromide.</div>
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<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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<div style="width: 90%;margin-left: 5%;margin-top: 2rem;padding-bottom: 4rem;color: #fff;display: flex;flex-direction: column;font-size: .9rem;">
<span>© 2022 - Content on this site is licensed under a <a href="" class="white_a">Creative Commons Attribution 4.0 International license</a>.</span>
<span>The repository used to create this website is available at <a href="" class="white_a">gitlab.igem.org/2022/xhd-wuhan-china</a>.</span>
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