Merge contribution

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@@ -56,23 +56,23 @@
             One can usually choose the hybrids showing melting temperatures 3 to 5 degrees celsius higher than that of
             the aptamer itself. However, that depends on the Kd of between the aptamer and the target protein. If the Kd
             is smaller that 10 -8 M (in the nM range), the hybrid with the melting temperature 10 degrees celsius higher
-            than that of the aptamer folding temperature itself can be chosen. Important part is the equilibrium between
-            the hybrids and the aptamer-target complex. If the melting temperatures still do not work, it is preferable
-            to try 2 bases or more deletion of both 5' and 3' end and those elongations. It also depends on the length
-            of the loop parts.<br/><br/>
-
+            than that of the aptamer folding temperature itself can be chosen. The important part is the equilibrium
+            between the hybrids and the aptamer-target complex. If the melting temperatures still do not work, it is
+            preferable to try 2 bases or more deletion of both 5' and 3' end and those elongations. It also depends on
+            the length of the loop parts.<br><br>
             Furthermore, the binding of biomarker with aptamer of Kd of nM levels can usually break the hybridization
             with Tm of 50 degree celsius. The binding with Kd of micro M can usually break the hybridization with Tm of
-            less than 40 degree celsius.
-            <br/><br/>
-
-            As our results show, the resulting aptamer probes were successful and produced satisfactory results that
-            lined up with our mathematical predictions, thus validating our design guidelines (please refer to our
-            results page).<br/><br/>
+            less than 40 degree celsius.<br><br>
+            We followed established protocols for ELONA, an ELISA assay modified to accommodate aptamers, in order to
+            determine the dissociation constant. We used a protocol modified from Sang et al to determine the Kd value
+            using the Hill equation, a Scatchard plot, and non-linear regression. However, the results were inconclusive
+            so we decided to find our Kd value through the 3D modeling software, UNAFold.<br><br>
+            The results demonstrated that the aptamer probes produced satisfactory results and aligned with our
+            mathematical predictions, thus validating our design guidelines. Please refer to our results page for
+            further details.<br><br>
             Lastly, we have devised a simple workflow for the detection and quantification of our biomarkers of interest
             using the Hill equation. For more information, please see our Modeling page.
-
-            <br/><br/></p>
+        </p>
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@@ -86,7 +86,7 @@
             future teams as reference when using our technology to determine the concentration of biomarkers in an
             unknown solution.<br/><br/>
 
-            For more information, please refer to our lab notebook and Modeling page.</p><br/><br/>
+            For more information, please refer to our lab notebook and Modeling page.</p>
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@@ -102,8 +102,8 @@
             practicality of our design in a clinical setting. Furthermore, we have also demonstrated the superior
             durability of aptamers over antibodies, thus also making a strong case for the feasibility of this
             technology.In short, we believe that the information gleaned from our experiments is extremely useful for
-            future teams hoping to use our aptamers in a project. <br/><br/>
-            For more information, please see our Parts page and Lab Notebook.
+            future teams hoping to use our aptamers in a project.
+
         </p>
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@@ -114,14 +114,13 @@
         </h1>
         <p>
             <img width="100%" src="https://static.igem.wiki/teams/4334/wiki/contribution1.png">
-            <br/>
-            We developed a short program that can take in inputs of the fluorescent value to quantify our biomarker
+            <br>We developed a short program that can take in inputs of the fluorescent value to quantify our biomarker
             concentration. We tested the program with our experimental results, and it functions properly. This would be
             useful for future teams working with FRET and a mechanism involving the interaction between two binding
             molecules, as one could merely change the initial values of Kd, L, and P0. This can be used in a variety of
             applications, such as the detection of another biomarker through its complementary aptamer and finding the
-            real
-            concentration of the biomarker-aptamer complex in solution.</p>
+            real concentration of the biomarker-aptamer complex in solution.
+        </p>
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