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  • 2024/bielefeld-cebitec
  • l-sanfilippo/bielefeld-ce-bi-tec-temp
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src/contents/Contribution/Wiki/wiki.tsx
View file @ 3fd292fd
import { Section } from "../../../components/sections";
import { Subesction } from "../../../components/sections";
import { WikiTabs } from "./wiki-tabs";
export function Wiki () {
return (
<Section title="Wiki" id="Wiki">
<Subesction title="Wiki Developement" id="Our Contributions2">
<WikiTabs></WikiTabs>
</Section>
</Subesction>
);
}
\ No newline at end of file
src/contents/Contribution/collabs.tsx
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import { Section } from "../../components/sections";
import { PDF } from "../../components/Pdfs";
import { Subesction } from "../../components/sections";
import { useNavigation } from "../../utils";
export function CollabContribution(){
export function CollabContribution(){
const {goToPagesAndOpenTab} = useNavigation();
return(
<Section title="Collaborations" id="Collaborations">
h
</Section>
<Subesction title="LNP Handbook" id="Our Collaborations2">
<p>In collaboration with the iGEM teams <a href="https://2024.igem.wiki/linkoping/">Linkoping</a>, <a href="https://2024.igem.wiki/patras/">Patras</a>, <a href="https://2024.igem.wiki/radboud-university/team">Radboud University</a> and <a href="https://2024.igem.wiki/termosz-selye-hun/">TERMOSZ-Selye-HUN</a>, we contributed a comprehensive overview of lipid-based delivery systems. Our contribution to the handbook aims to provide future iGEM teams with a deeper understanding of LNP (Lipid Nanoparticle) development. By bringing together diverse teams and innovative solutions, we strive to make a positive impact on the emerging field of LNP design. Our goal is to enhance the accessibility of various LNP formulations and adapt this promising technology for a wide range of therapeutic applications. </p>
<p>Download our LNP Hanbook that was created in <a onClick={() => goToPagesAndOpenTab('colls2024', '/human-practices')}>cooperation</a> with the team <a href="https://2024.igem.wiki/linkoping/">Linköping</a>.</p>
<PDF link="https://static.igem.wiki/teams/5247/pdfs/liposomes-handbook.pdf" name="liposomes-handbook.pdf"/>
</Subesction>
)
}
\ No newline at end of file
src/contents/Contribution/contribution.tsx
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import { GuidePDF } from "../../components/Pdfs";
import { Section, Subesction } from "../../components/sections";
import { useTabNavigation } from "../../utils/TabNavigation";
import { MeetUP } from "./BFH/bfh";
import { CollabContribution } from "./collabs";
......@@ -8,10 +10,38 @@ export function Contribution() {
useTabNavigation();
return (
<>
<Section title="Abstract" id="Abstract">
<p>The BFH European Meet-Up showcased significant contributions to the iGEM community through extensive collaborations and innovative advancements. Our key contributions include the development of the BFH Guideline in partnership with iGEM teams Hamburg and Frankfurt, providing a blueprint for future Meet Ups, and a collaborative video podcast with iGEM Münster, offering insights into team growth. </p>
<p>Additionally, we contributed to the LNP Handbook with teams from <a href="https://2024.igem.wiki/linkoping/">Linkoping</a>, <a href="https://2024.igem.wiki/patras/">Patras</a>, <a href="https://2024.igem.wiki/radboud-university/team">Radboud University</a>, and <a href="https://2024.igem.wiki/termosz-selye-hun/">TERMOSZ-Selye-HUN</a>, advancing lipid-based delivery systems for therapeutic use. </p>
<p>On the scientific front, we developed practical tools like a collection of optimized protocols, the PreCL reporter system for Prime Editing, and the innovative PrimeGuide Prime Editing technology, enhancing research efficiency and precision. Our AirBuddy lipid nanoparticle system represents a breakthrough in gene therapy, offering a safer and more effective method for lung-specific RNA and DNA delivery. These collaborations and innovations will have a lasting impact, supporting future iGEM teams and driving forward synthetic biology research. </p>
</Section>
<MeetUP/>
<SafetyContribution/>
<CollabContribution/>
<Wiki/>
<Section title="Our Collaborations" id="Our Collaborations">
<Subesction title="MeetUp Guideline" id="Our Collaborations1">
<GuidePDF/>
<p>In cooperation with the iGEM teams Hamburg and Frankfurt, we have developed a comprehensive guideline for the organization of a meet-up. This guideline serves as a template for other iGEM teams and provides a complete overview of all essential aspects that should be considered when planning and organizing a successful meet-up. It covers all the necessary content, from initial brainstorming to practical implementation, and is designed to help teams organize their own events smoothly. </p>
<p>Additionally, in collaboration with iGEM Münster, we created a video podcast that summarizes the developments and feedback from the participating teams and reflects on both Meet-ups. It was particularly interesting to observe the growth of the team members and the progress of their projects. The video podcast will be featured on the Wiki page of Team Münster and will also be included here after the Wiki freeze. </p>
</Subesction>
<CollabContribution/>
</Section>
<Section title="Our Contributions" id="Our Contributions">
<SafetyContribution/>
<Wiki/>
</Section>
<Section title="Conclusion" id="Conclusion">
<p>Throughout the BFH European Meet-Up, we actively engaged in meaningful collaborations and made significant contributions to the iGEM community. In partnership with iGEM teams Hamburg and Frankfurt, we developed the BFH Guideline, a comprehensive resource designed to help future teams successfully organize Meet Ups. Our collaboration with iGEM Münster resulted in a video podcast that reflects on the growth and progress of participating teams, providing valuable insights for future competitions. </p>
<p>Additionally, we worked alongside teams from <a href="https://2024.igem.wiki/linkoping/">Linkoping</a>, <a href="https://2024.igem.wiki/patras/">Patras</a>, <a href="https://2024.igem.wiki/radboud-university/team">Radboud University</a>, and <a href="https://2024.igem.wiki/termosz-selye-hun/">TERMOSZ-Selye-HUN</a> to contribute to the LNP Handbook, advancing knowledge in lipid-based delivery systems for therapeutic applications. </p>
<p>On the scientific front, we created a collection of protocols, the PreCL reporter system, and the innovative PrimeGuide Prime Editing technology, all of which aim to enhance research efficiency and precision. Our AirBuddy nanoparticle system for lung-specific delivery further exemplifies our commitment to advancing gene therapy solutions. These collaborations and contributions not only enriched the event but will continue to have a lasting impact on future iGEM teams and synthetic biology research. </p>
</Section>
</>
);
}
......
src/contents/Contribution/patient-contribution.tsx 0 → 100644
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import { H4 } from "../../components/Headings";
import { PDF, TwoLinePDF } from "../../components/Pdfs";
export function PatientContribution(){
return(
<>
<div className='row align-items-center'>
<div className='col '>
<H4 text="Patient consent form"/>
<TwoLinePDF link="https://static.igem.wiki/teams/5247/pdfs/patienteneinwilligung-mustervorlage-igem-2.pdf" name="patienteneinwilligung-mustervorlage-igem-2.pdf"/>
</div>
<div className='seperator-2 col-2'>
</div>
<div className='col '>
<H4 text="Questionnaire for sample collection "/>
<TwoLinePDF link="https://static.igem.wiki/teams/5247/pdfs/translated-questionnaire-cf-patients.pdf" name="translated-questionnaire-cf-patients.pdf"/>
</div>
<p>When working with primary cultures, it is extremely important to consider the bioethical aspects of the project. To address this, we sat down with the Ethics Officer at Bielefeld University, Dr. Berens, and discussed the matter with her. As a result, we created a patient consent form for the donors of primary cells, which we also want to present as a template for future German iGEM teams. <b>However, we want to emphasize that it is not guaranteed to be comprehensive, nor does it have any legal approval.</b></p>
<H4 text="Hygiene Concept"/>
<PDF link="https://static.igem.wiki/teams/5247/pdfs/final-hygiene-concept.pdf" name="final-hygiene-concept.pdf"/>
</div>
</>
)
}
\ No newline at end of file
src/contents/Contribution/safety-contribution.tsx
View file @ 3fd292fd
import { Section } from "../../components/sections";
import { H4 } from "../../components/Headings";
import { PDF } from "../../components/Pdfs";
import { Subesction } from "../../components/sections";
import { PatientContribution } from "./patient-contribution";
export function SafetyContribution(){
return(
<Section title="Safety Guidelines" id="Safety Guidelines">
h
</Section>
<>
<Subesction title="Biosafety & Security" id="Our Contributions1" >
<H4 text="Primary Culture Safety Guideline"/>
<p>In the early phases of our project, we encountered several challenges while working to bring it together. We quickly decided to focus on human biomaterials, specifically cultivating primary human nasal epithelial cells from both Cystic Fibrosis (CF) patients and wild-type controls. To ensure compliance, we carefully evaluated the safety regulations of our institution and government. Our contribution includes three key elements: </p>
<ol>
<li> A guideline outlining the proper handling of biomaterials according to BSL2 standards, along with additional safety measures to ensure secure experimental design. </li>
<li> A clinical trial-style questionnaire designed to assess the medical history of participants donating nasal epithelial cells. </li>
<li> A hygiene protocol aimed at improving safety and cleanliness standards in various institutions and sanitary facilities. </li>
</ol>
<PDF link="https://static.igem.wiki/teams/5247/pdfs/primary-culture-guideline.pdf" name="primary-culture-guideline.pdf"/>
<PatientContribution/>
<p>By contributing these frameworks, we enable future iGEM teams to overcome potential limitations that may arise during project development. These resources provide clear guidelines and tools to ensure safety, regulatory compliance, and efficient workflow, allowing teams to focus on advancing their research without facing similar challenges. </p>
</Subesction>
</>
)
}
\ No newline at end of file
src/contents/Home Folder/svg-one.tsx 0 → 100644
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src/contents/Home.tsx
View file @ 3fd292fd
import { FadeIn } from "../components/FadeIn";
import { Breathe } from "../components/Breathe";
import { Problems } from "../components/Problems";
import { AirbuddyAnim } from "../components/AirbuddyAnim";
import { PrimeGuideAnimOne, PrimeGuideAnimTwo } from "../components/PrimeGuide";
import { useTabNavigation } from "../utils/TabNavigation";
import { H5 } from "../components/Headings";
import PreCyse from "../components/precyse";
import { useNavigation } from "../utils";
import { OurLink } from "../components/Link";
export function Home() {
useTabNavigation();
const {goToPageAndScroll} = useNavigation();
/* */
return (
<>
<div className="col">
<div className="col">
<div className="col header-container landing-page-header" id="landing-page-header">
<div className="row align-items-center">
<div className="col">
<img src="https://static.igem.wiki/teams/5247/thaw/2024-preise.webp" id="lpbild"/>
</div>
<div className="col-3">
<img src="https://static.igem.wiki/teams/5247/thaw/gold-medaille-wiki.webp" />
</div>
</div>
<br/>
<h3 style={{textAlign: "center"}}>Our Project, Our Victory</h3>
<h4 style={{textAlign: "center"}}>Gold Medal for Excellence in Synthetic Biology and Engineering Success as one of the best therapeutic projects</h4>
<p style={{textAlign: "center"}}>A true honor for our <b>cutting-edge</b> contributions to the world of synthetic biology and <b>engineering breakthroughs</b>.</p>
<br/>
</div>
</div>
<div className="row align-items-center">
<div className="col center">
<img style={{margin: "auto"}} height="400px" className="center" src="https://static.igem.wiki/teams/5247/thaw/best-integrated-human-pratices.webp" />
</div>
<div className="col">
<img style={{margin: "auto"}} height="400px" className="center" src="https://static.igem.wiki/teams/5247/thaw/best-presentation-award.webp" />
</div>
<div className="col">
<img style={{margin: "auto"}} height="400px" className="center" src="https://static.igem.wiki/teams/5247/thaw/safety-and-security-neu.webp" />
</div>
</div>
<div className="row align-items-center" style={{marginBottom: "none !important", paddingBottom: "0px !important"}}>
<div className="col" style={{marginBottom: "none !important", paddingBottom: "0px !important"}}>
<h3 style={{textAlign: "center"}}>Best Integrated Human Practices</h3>
</div>
<div className="col" style={{marginBottom: "none !important", paddingBottom: "0px !important"}}>
<h3 style={{textAlign: "center"}}>Best Presentation</h3>
</div>
<div className="col" style={{marginBottom: "none !important", paddingBottom: "0px !important"}}>
<h3 style={{textAlign: "center"}}>Safety and Security Award</h3>
</div>
</div>
<div className="row align-items-center" >
<div className="col">
<p style={{textAlign: "center"}}>Ethical, impactful, and groundbreaking science</p>
</div>
<div className="col">
<p style={{textAlign: "center"}}>Ability to communicate complex science with clarity and impact</p>
</div>
<div className="col">
<p style={{textAlign: "center"}}>Unmatched safety standards, ensuring innovation with responsibility</p>
</div>
</div>
<br/><br/>
<p style={{textAlign: "center"}}>In 2024, our groundbreaking work in <b>synthetic biology</b> and <b>medical engineering</b> was recognized with <b>multiple prestigious awards</b>,
marking a monumental achievement in our journey. These <b>honors</b> are not just trophies — they represent the <b>passion</b>, <b>innovation</b>,
and excellence embedded in every aspect of our project.</p>
<p style={{textAlign: "center"}}>These <b>honors</b> don’t just validate our science — they affirm our <b>vision</b> for a healthier, smarter, and more sustainable future.
</p>
<h4 style={{textAlign: "center"}}>Experience the Journey </h4>
<p style={{textAlign: "center"}}>Dive deeper into our award-winning work. Watch our <b>epic presentation</b> and explore the full scope of our project.
</p>
<p style={{textAlign: "center"}}>Check out the videos and more to see how we are <b>pushing the boundaries</b> of what's possible:
</p>
<p style={{textAlign: "center"}}><OurLink text="Our Project Overview" path="description" scrollToId="Abstract"/></p>
<p style={{textAlign: "center"}}>Award-Winning Presentation Video</p>
<div style={{textAlign: "center"}} className="row align-items-center">
<div className="col">
<iframe title="Bielefeld-CeBiTec: Next-Generation Prime Editing as Cystic Fibrosis Gene Therapy (2024) - Team Presentation [English]" width="560" height="315" src="https://video.igem.org/videos/embed/479e7f99-6931-47bc-9193-d4367beba4f2" frameBorder="0" allowFullScreen sandbox="allow-same-origin allow-scripts allow-popups allow-forms"></iframe>
</div>
</div>
{/* <div className="col">
<div className="col header-container landing-page-header" id="landing-page-header-2">
<div className="row align-items-center">
<div className="col header-button-row">
<div><H5 text="Take a tour through our highlights:"/> </div>
<p>
&#8594; <a href="description?scrollTo=Abstract">What is <PreCyse/>? </a> <br/> <br/>
&#8594; <a href="engineering?tab=tab-our-cycle&scrollTo=ourcycle">What is our strategy?</a> <br/> <br/>
&#8594; <a href="materials-methods?scrollTo=introduction">How did we do this?</a> <br/> <br/>
&#8594; <a href="results?scrollTo=experimental-design">What did we archieve?</a> <br/> <br/>
&#8594; <a href="parts?scrollTo=Parts Collection2">What parts are we contributing?</a> <br/> <br/>
&#8594; <a href="judging?scrollTo=OverviewH">What are our special awards?</a>
</p>
</div>
<div className="col header-button-row">
<p>
<div className="col">
<button onClick={() => goToPageAndScroll("scrollstart", "")}> <b>SCROLL</b> to see introductory animation.</button>
</div>
</p>
<p>
<div className="col">
<button onClick={() => goToPageAndScroll("Abstract", "/description")} > <b>CLICK</b> to go directly to our Project Description.</button>
......@@ -25,78 +107,36 @@ export function Home() {
</p>
<p>
<div className="col">
<button onClick={() => goToPageAndScroll("sciency", "")}> <b>SKIP</b> to jump straight to the scienicy part of the animation.</button>
<button onClick={() => goToPageAndScroll("sciency", "")}> <b>JUMP</b> straight to the science part of the animation.</button>
</div>
</p>
<p>
<div className="col">
<button onClick={() => goToPageAndScroll("scrollstart", "")}> <b>SCROLL</b> to see whole animation.</button>
</div>
</p>
</div>
<div className="col header-button-row">
<div><H5 text="Or take a tour through our highlights:"/> </div>
<p>
&#8594; <a href="description?scrollTo=Abstract">What is <PreCyse/>? </a> <br/> <br/>
&#8594; <a href="engineering?tab=tab-our-cycle&scrollTo=ourcycle">What is our strategy?</a> <br/> <br/>
&#8594; <a href="experiments?tab=heading">How did we do this?</a> <br/> <br/>
&#8594; <a href="results?scrollTo=experimental-design">What did we archieve?</a> <br/> <br/>
&#8594; <a href="parts?scrollTo=Parts Collection2">What parts are we contributing?</a> <br/> <br/>
&#8594; <a href="judging?scrollTo=OverviewH">What are our special awards?</a>
</p>
</div>
</div>
</div>
</div>
<div className="row mt-4">
<div className="col" id="erstecol">
<div className="col">
<div style={{'position': 'relative',
'zIndex': '1',
'top': '10vh',
'left': '0vw',
'scale': '0.25'}}>
<div id="scrollstart"></div>
<img src="https://static.igem.wiki/teams/5247/landing/indicator.webp"></img>
</div>
</div> */}
{/* Spacing Block */}
<div className='col' style={{ 'height': '150vh' }}></div>
<FadeIn filepath="https://static.igem.wiki/teams/5247/thaw/lp1.webp" bg='https://static.igem.wiki/teams/5247/thaw/lp1.webp'></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/thaw/lp2.webp" bg='https://static.igem.wiki/teams/5247/thaw/lp2.webp'></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/thaw/lp3.webp" bg='https://static.igem.wiki/teams/5247/thaw/lp3.webp'></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/thaw/lp4.webp" bg='https://static.igem.wiki/teams/5247/thaw/lp4.webp'></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/thaw/lp5.webp" bg='https://static.igem.wiki/teams/5247/thaw/lp5.webp'></FadeIn>
<Breathe></Breathe>
<Problems></Problems>
{/* Spacing Block */}
<div className='col' style={{ 'height': '150vh' }}></div>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/gifs/200k-anim-transparent-bg.gif" bg='https://static.igem.wiki/teams/5247/landing/backgrounds/background-1-text.webp'></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/gifs/oneintwenty-no-bg-cropped.gif" bg=''></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/gifs/2k-anim-transparent-bg.gif" bg='https://static.igem.wiki/teams/5247/landing/backgrounds/background-2-text.webp'></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/gifs/3k-anim-transparent-bg.gif" bg='https://static.igem.wiki/teams/5247/landing/backgrounds/background-3-text.webp'></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/gifs/treatment-2.gif" bg=''></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/airbuddy.gif" bg=''></FadeIn>
<AirbuddyAnim></AirbuddyAnim>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/primeguide.webp" bg=''></FadeIn>
<PrimeGuideAnimOne></PrimeGuideAnimOne>
<PrimeGuideAnimTwo></PrimeGuideAnimTwo>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/precyse.gif" bg=''></FadeIn>
<FadeIn filepath="https://static.igem.wiki/teams/5247/landing/gifs/proofofconcept.gif" bg=''></FadeIn>
{/* Spacing Block */}
<div className='col' style={{ 'height': '100vh' }}></div>
<img src="https://static.igem.wiki/teams/5247/landing-page/lp-2.svg"/>
<div className="row">
<div className="col button-x">
<button onClick={() => goToPageAndScroll("Approach2H", "/description")} > Lung-Specific Lipid Nanoparticle</button>
</div>
<div className="col button-x">
<button onClick={() => goToPageAndScroll("Approach1H", "/description")} > Next-Generation Prime Editing Technology
</button>
</div>
</div>
</div>
</div>
</>
);
}
src/contents/Human Practices/.DS_Store 0 → 100644
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src/contents/Human Practices/Conclisuin.tsx 0 → 100644
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src/contents/Human Practices/Further Engagement/Collaborations.tsx
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import { ButtonOne } from "../../../components/Buttons";
import { H4 } from "../../../components/Headings";
import { LoremMedium } from "../../../components/Loremipsum";
export function HPCollabs(){
import { H5, H4 } from "../../../components/Headings";
import { PDF } from "../../../components/Pdfs";
import { useTabNavigation } from "../../../utils/TabNavigation";
import { ButtonOneWithScroll } from "../../../components/Buttons";
return (
export function HPCollabs(){
useTabNavigation();
return(
<div className="col">
<div className="row align-items-center" style={{marginTop: "5vh", marginBottom: "5vh"}}>
<div className="col">
<ButtonOne openclass="coll-cycletab" text="Overview" open="coll-overview"></ButtonOne>
</div>
<div className="col">
<ButtonOne openclass="coll-cycletab" text="Collabs in 2024" open="colls2024"></ButtonOne>
</div>
<div className="col">
<ButtonOne openclass="coll-cycletab" text="LNP Handbook" open="Handbook"></ButtonOne>
</div>
<div className="row align-items-center" >
<div className="col ">
<ButtonOneWithScroll openclass="coll-cycletab" text="Overview" open="coll-overview" scrollId="coll-heading"/>
</div>
<div className="col ">
<ButtonOneWithScroll openclass="coll-cycletab" text="Collabs in 2024" open="colls2024" scrollId="colls2024-heading"/>
</div>
<div className="col ">
<ButtonOneWithScroll openclass="coll-cycletab" text="LNP Handbook" open="Handbook" scrollId="Handbook-heading"/>
</div>
</div>
<div id="coll-overview" className="coll-cycletab" style={{display: "block"}}>
<H4 id="coll-overview-heading" text=""/>
<LoremMedium/>
<H4 id="coll-heading" text="Collaborations as part of a integrated human practice - but why?"/>
<p>Collaboration is at the heart of innovative science, especially in projects with significant societal and clinical implications such as ours. By actively engaging with stakeholders across disciplines, we ensured that our work addressed real-world needs and took ethical and practical considerations into account.</p>
<p>For example, partnerships with patients and advocacy groups provided a deeper understanding of living with CF and shaped our patient-centered approach. Discussions with medical professionals highlighted the clinical challenges and regulatory requirements we needed to address. Collaborating with other iGEM teams allowed us to share methodologies, refine protocols, and integrate diverse expertise into our project.</p>
<p>These interactions didn't just guide the scientific direction of our work - they emphasised the wider impact of synthetic biology. By combining our technical efforts with stakeholder input, we ensured that our solutions were not only innovative, but also feasible, ethical and impactful. Collaboration thus became the foundation of a truly integrated human practice, bridging the gap between science, society and application.</p>
<H4 id="coll-our-heading" text="Our Collaborations"/>
<p>Collaboration was key to the success of our project. By engaging with patients, advocacy groups, medical professionals, and other iGEM teams, we gained valuable insights into the clinical, ethical, and practical challenges of addressing Cystic Fibrosis. These partnerships shaped our patient-centered approach, refined our methodologies, and ensured our solutions are impactful and feasible. Through collaboration, we bridged the gap between science and society, creating a truly integrated human practice.
</p>
</div>
<div id="colls2024" className="coll-cycletab" style={{display: "none"}}>
<H4 id="colls2024-heading" text="Collaboration with Lingköping"/>
<H4 text="Home University of Linköping"/>
<H4 id="colls2024-heading" text="Home University of Linköping"/>
<p>The University of Linköping is one of the bigger universities located in Sweden. Since its inception in 1975, it has become a very innovative and highly renowned institution. </p>
<p>The project of Linköpings iGEM 2024 team: (description taken from their website)</p>
<p>“The product composition will depend on the enzyme missing or malfunctioning in each of the disease types. We’re going to target autosomal recessive congenital ichthyosis, as this is the one our friend is struggling with. We will focus on the consequences of mutations in three different genes (TGM1, ALOXE3, ALOX12B) that can underlie this condition [5]. However, if our approach turns out to be successful, after some adjustments, the protocol could be applied for the remaining types of the disease as well. First of all, we’ll engineer E. coli to produce the chosen enzymes encoded by the corresponding genes we chose: transglutaminase 1, Epidermis-type lipoxygenase 3, Arachidonate 12-lipoxygenase and then we will purify them from the bacteria. Once this system is established and optimized, we’ll proceed to design a functioning delivery system that we will encapsulate the enzymes in. We have decided to produce modulated liposomes that will be able to keep the enzymes active while transporting them. Once the target skin layer is reached, the liposomes will fuse with the membranes of the cells of interest, delivering the product to its final destination. Functioning liposomes packed with the produced enzymes will then be incorporated into a suitable medium to facilitate the topical application for the patients”. </p>
<H4 text="iGEM team Liu project our idea"/>
<p>We first made contact with the team of LIU via email, due to both our teams’ interest in working with LNP based delivery systems. It rapidly became apparent that our two teams could benefit from a corporation especially since the team of LiU was working on an LNP handbook at the time.</p>
<H5 text="Biosafety and Security"/>
<p>Early in our project, we faced challenges working with human biomaterials, particularly cultivating primary human nasal epithelial cells from both CF patients and controls. To address these, we made three key contributions:</p>
<ol>
<li>A guideline for handling biomaterials in compliance with BSL2 standards.{/* [Link guideline] */}</li>
<li>A clinical trial-style questionnaire to assess donor medical history.{/* [Link Link questionaire] */}</li>
<li>A hygiene protocol to improve safety and cleanliness in research facilities.{/* [Link hygiene protocoll] */}</li>
</ol>
<p>
These frameworks ensure that future iGEM teams can overcome similar challenges, ensuring safety and regulatory compliance while streamlining their workflow.
We contributed an extensive collection of optimized protocols for future iGEM teams, integrating our experiences to make synthetic biology more accessible. By embedding safety standards, we enable teams to confidently work with human biomaterials, ensuring regulatory compliance and efficient progress.
</p>
<H5 text="PreCL Reporter System "/>
<p>To test Prime Editing systems targeting the CF-specific delF508 mutation, we developed the PreCL reporter system [Link engineering of PreCL], which offers high sensitivity, minimal noise, and precise fluorescence detection. This versatile tool, adaptable for CRISPR and base editing, enhances the precision of genetic research, particularly in CF studies.
We optimized pegRNA design{/* [linkpegrna] */} by incorporating the TevoPreQ1 RNA motif, improving stability and Prime Editing efficiency. Our innovations, including silent edits and fine-tuned sequences, boost editing accuracy, providing a robust tool for genetic research. </p>
<H5 text="Prime Editing Technology PrimeGuide & Lipid Nanoparticle System AirBuddy "/>
<p>Our PrimeGuide{/* [link] */}system introduces a novel eukaryotic RNA-binding DNA-nickase, a smaller alternative to Cas9. Enhanced with a more efficient Reverse Transcriptase and optimized RNA-binding proteins, this advancement improves Prime Editing accuracy and safety for genetic mutation correction.
We developed AirBuddy{/* [link] */}, a lung-specific RNA/DNA delivery system optimized for gene therapies targeting lung diseases. With low cytotoxicity, efficient cellular uptake, and cost-effective storage, AirBuddy revolutionizes lung disease treatments by providing a safer and more effective delivery method. </p>
<H5 text="Wiki Development"/>
<p>To support future iGEM teams, we developed troubleshooting guides for HTML and CSS{/* [link] */}, making wiki development more accessible and easier to manage.
Through these contributions, we provide valuable tools and frameworks to advance synthetic biology, ensuring safer, more efficient research and therapeutic development for the iGEM community. </p>
</div>
<div id="Handbook" className="coll-cycletab" style={{display: "none"}}>
<H4 id="Handbook-heading" text=""/>
<LoremMedium/>
<H4 id="Handbook-heading" text="Hanbook for download"/>
<PDF link="https://static.igem.wiki/teams/5247/pdfs/liposomes-handbook.pdf" name="liposomes-handbook.pdf"/>
</div>
</div>
)
}
\ No newline at end of file
src/contents/Human Practices/Further Engagement/FurtherEngagement.tsx
View file @ 3fd292fd
import { Section, Subesction } from "../../../components/sections";
import { useTabNavigation } from "../../../utils/TabNavigation";
import { HPCollabs } from "./Collaborations";
import { HPEducation } from "./Education";
import { HPEntrepreneur } from "./Entrepreneurship";
......@@ -6,7 +7,7 @@ import { HPOutreach } from "./Outreach";
import { HPPartnerships } from "./Partnerships";
export function HPFurtherEngagement(){
useTabNavigation();
return(
<Section title="Further Engagement" id="Further Engagement">
<Subesction title="Education" id="Further Engagement1">
......
src/contents/Human Practices/Further Engagement/Partnerships.tsx
View file @ 3fd292fd
import { H4 } from "../../../components/Headings";
import { useTabNavigation } from "../../../utils/TabNavigation";
export function HPPartnerships(){
useTabNavigation();
return(
<div className="col">
<H4 text="CF Vests"></H4>
<p>CF Vests Worldwide is a dedicated charity organization committed to providing life-saving vest equipment to those in need, regardless of their financial situation. But they can't do it alone — they need your support. Help us make a difference! By donating to CFVWW, you can directly impact the lives of cystic fibrosis patients, giving them the chance to breathe easier and live fuller lives. Every contribution counts.</p>
<p><strong>Join us in the fight against cystic fibrosis.</strong> <a href="https://donorbox.org/igem"> Donate today</a> and help us bring hope, one vest at a time! Together, we can change lives.</p>
<p>CF Vests Worldwide is a dedicated charity organization committed to providing life-saving vest equipment to those in need, regardless of their financial situation. But they can't do it alone — they need your support. Help us make a difference! By donating to CFVWW, you can directly impact the lives of Cystic Fibrosis patients, giving them the chance to breathe easier and live fuller lives. Every contribution counts.</p>
<p><strong>Join us in the fight against Cystic Fibrosis.</strong> <a href="https://donorbox.org/igem"> Donate today</a> and help us bring hope, one vest at a time! Together, we can change lives.</p>
</div>
)
}
\ No newline at end of file
src/contents/Human Practices/IHP.tsx
View file @ 3fd292fd
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