From f9702426b1c8abbfba04ff2b734e991b760bcf1e Mon Sep 17 00:00:00 2001
From: HouTeng Chan <ht-chen21@mails.tsinghua.edu.cn>
Date: Sat, 28 Sep 2024 09:13:14 +0000
Subject: [PATCH] Update file engineering.html
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wiki/pages/engineering.html | 6 +++---
1 file changed, 3 insertions(+), 3 deletions(-)
diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html
index a912ccb4..243efe42 100644
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@@ -207,7 +207,7 @@
<h5>Design</h5>
<p>After conducting a literature review, we decided to introduce the tetrathionate sensor TtrSR into yeast cells to serve as a receptor for IBD.</p>
<h5>Build</h5>
- <p>After confirming the sequence, we designed plasmids that can construct the tetrathionate sensor TtrSR system within Saccharomyces cerevisiae, each carrying the URA3 and HIS3 genes as selection markers, as shown in the figure below.</p>
+ <p>After confirming the sequence, we designed plasmids that can construct the tetrathionate sensor TtrSR system within Saccharomyces cerevisiae, each carrying the URA3 and HIS3 genes as selection marers, as shown in the figure below.</p>
<div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
<img src="https://static.igem.wiki/teams/5187/wiki-engineering-fig/figure11.png" alt="ibd_figure" class="shadowed-image" style="width: 50%; max-width: 500px;">
@@ -218,10 +218,10 @@
<p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 12 pESC-SV40-ttrR-PttrB-EGFP plasmid</p>
</div>
<h5>Test</h5>
- <p>We induced the transformed yeast with 1mM K₂O₆S₄ or without K₂O₆S₄ (as a control) and measured the expression level of EGFP after 12 hours of induction. The final results showed that after constructing the tetrathionate sensor TtrSR system in yeast cells, the cells were able to sense the IBD signal tetrathionate and activate the expression of downstream genes. However, compared to the control group, there was no significant increase in the expression level of the downstream protein. Although the downstream protein was expressed in response to the IBD signal, the expression level still needs to be improved.</p>
+ <p>We induced the transformed yeast with 1mM K<sub>2</sub>O<sub>6</sub> S<sub>4</sub> or without K<sub>2</sub>O<sub>6</sub> S<sub>4</sub> (as a control) and measured the expression level of EGFP after 12 hours of induction. The final results showed that after constructing the tetrathionate sensor TtrSR system in yeast cells, the cells were able to sense the IBD signal tetrathionate and activate the expression of downstream genes. However, compared to the control group, there was no significant increase in the expression level of the downstream protein. Although the downstream protein was expressed in response to the IBD signal, the expression level still needs to be improved.</p>
<div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
<img src="https://static.igem.wiki/teams/5187/wiki-engineering-fig/figure13.png" alt="ibd_figure" class="shadowed-image" style="width: 50%; max-width: 500px;">
- <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 13 Tetrathionate induction experiment (s: control group without inducer; s+: K₂O₆S₄ added)</p>
+ <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 13 Tetrathionate induction experiment (s: control group without inducer; s+: K<sub>2</sub>O<sub>6</sub> S<sub>4</sub> added)</p>
</div>
<h5>Learn</h5>
<p>Regarding this phenomenon, after discussion, we hypothesize that it may be due to the low expression levels of TtrS and TtrR, or it could be that the membrane protein TtrS, originally from prokaryotic cells, is unable to effectively localize and anchor to the membrane after being expressed in yeast cells. We need to further optimize the relevant sequences.</p>
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