From bec0c69d039f32496417015da3d93a550b379d90 Mon Sep 17 00:00:00 2001
From: HouTeng Chan <ht-chen21@mails.tsinghua.edu.cn>
Date: Tue, 24 Sep 2024 04:49:57 +0000
Subject: [PATCH] Update file contribution.html
---
wiki/pages/contribution.html | 88 +++++++++++++++++++-----------------
1 file changed, 47 insertions(+), 41 deletions(-)
diff --git a/wiki/pages/contribution.html b/wiki/pages/contribution.html
index a28a741d..c25b5cb8 100644
--- a/wiki/pages/contribution.html
+++ b/wiki/pages/contribution.html
@@ -36,11 +36,8 @@
{% block page_content %}
<div class="sidebar">
<ul>
- <li><a href="#description">General Description</a></li>
- <li><a href="#topic1">Topic1</a></li>
- <li><a href="#topic2">Topic2</a></li>
- <li><a href="#topic3">Topic3</a></li>
- <li><a href="#topic4">Topic4</a></li>
+ <li><a href="#Parts">Parts</a></li>
+ <li><a href="#Treatment for IBD">Treatment for IBD</a></li>
</ul>
</div>
@@ -55,61 +52,70 @@
<div class="row mt-4">
<div class="col-lg-12">
- <h2 id="description">
- <h2>General Description</h2>
<hr>
- <p>Inflammatory Bowel Disease (IBD) is an indeterminate colitis and autoimmune inflammatory bowel disease that involves the ileum, rectum, and colon. Due to its unknown pathogenesis, it is difficult to cure and prone to relapse, which is then referred to as the “green cancerâ€. As the incidence of IBD is increasing globally, the poor therapeutic experience, which the patients are required to receive long-term hormone injection and biologic combination therapy, is determined to seek a breakthrough. Our team stands to develop a more favorable treatment experience. Relevant studies have shown that lactic acid has an inhibitory effect on abnormally activated dendritic cells and cytotoxic T-cells in autoimmune diseases, and thus is expected to improve the symptoms of IBD patients. Based on the research, we designed a yeast engineered bacterium. We introduced a muscone detecting switch in the engineered yeast and activated the downstream pathway to express lactate dehydrogenase to produce lactic acid. Ultimately, the engineered bacteria will colonize the small intestine, enabling the patient to treat IBD by inhaling muscone gas in vitro therefore induces the yeast to synthesize a low amount of stabilized lactic acid in vivo. Aroma therapy has the potential to be a new trend in long-term treatment for IBD as it can reduce the discomfort of treatment and improve the long-term treatment experience. </p>
+ <p>As we developed a brand-new way to alleviate IBD utilizing muscone molecular switch to induce lactic acid secretion, our contribution for future iGEM teams can be discussed in 2 sections: Parts and Treatment for IBD.</p>
</div>
- <div class="image-container">
- <img src="https://static.igem.wiki/teams/5187/figure/ibd-figure.jpg" alt="ibd_figure" class="shadowed-image">
</div>
- </div>
-
+
<div class="row mt-4">
<div class="col-lg-12">
- <h2 id="topic1">
- <h2>Topic1</h2>
- <hr>
- <p>Tsinghua University is a public university in Haidian, Beijing. It is affiliated with and funded by the Ministry of Education of China. The university is part of Project 211, Project 985, and the Double First-Class Construction. It is also a member of the C9 League. Tsinghua University's campus is situated in northwest Beijing, on the site of the former imperial gardens of the Qing dynasty. Currently, the university has 21 schools and 59 departments, with faculties in science, engineering, humanities, law, medicine, history, philosophy, economics, management, education and art.</p>
- <p>Notable alumni who have held senior positions in Chinese politics include current general secretary and president of China, Xi Jinping, former general secretary and president of China Hu Jintao, former chairman of the National People's Congress Wu Bangguo, former premier Zhu Rongji, and the former first vice premier Huang Ju. This also includes politicians like Wu Guanzheng, former governor of the People's Bank of China Zhou Xiaochuan, former minister of finance Lou Jiwei, general Sun Li-jen, Liang Qichao, and more. Since 2016, Tsinghua graduates who have political prominence are disproportionately greater in number than graduates of other famous universities.</p>
+ <h2 id="Parts">
+ <h2>Parts</h2>
+ <hr>
+ <div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
+ <img src="https://static.igem.wiki/teams/5187/wiki-contribution-fig/1.jpg" alt="ibd_figure" class="shadowed-image" style="width: 90%; max-width: 800px;">
+ <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 1 Overall Design</p>
+ </div>
</div>
</div>
-
+
<div class="row mt-4">
<div class="col-lg-12">
- <h2 id="topic2">
- <h2>Topic2</h2>
- <hr>
- <p>Tsinghua University engages in extensive research and offers 51 bachelor's degree programs, 139 master's degree programs, and 107 doctoral programs through 20 colleges and 57 departments covering a broad range of subjects, including science, engineering, arts and literature, social sciences, law, medicine. Along with its membership in the C9 League, Tsinghua University affiliations include the Association of Pacific Rim Universities, a group of 50 leading Asian and American universities, Washington University in St. Louis's McDonnell International Scholars Academy, a group of 35 premier global universities, and the Association of East Asian Research Universities, a 17-member research collaboration network of top regional institutions. Tsinghua is an associate member of the Consortium Linking Universities of Science and Technology for Education and Research (CLUSTER). Tsinghua is a member of a Low Carbon Energy University Alliance (LCEUA), together with the University of Cambridge and the Massachusetts Institute of Technology (MIT).</pp>
- <p>School of Life Sciences was first established in 1926 under the name Department of Biology. Botanist Qian Chongshu took up the first dean.During the nationwide reorganization of universities in the early 1950s, the Department of Biology was merged into other universities, namely Peking University etc., resulting in a vacancy in the field of biological research in Tsinghua for almost 30 years.In June 1984, decisions were made about the reestablishment of the Department of Biology, and the department officially reopened in September. During the reestablishment the Department of Biology of Peking University, the Institute of Biophysics of Chinese Academy of Sciences, and many other institutes as well as biologists provided valuable support and help. The department changed its name to the current name in September 2009. As of 2013, structural biologist and foreign associate of National Academy of Sciences of United States Dr. Wang Hongwei (王å®ä¼Ÿ) is the current dean of School of Life Sciences. The school currently has 129 professors and employees, around 600 undergraduates (including the candidates of Tsinghua University – Peking Union Medical College joint MD program).</p>
+ <h3>Muscone molecular switch in Saccharomyces cerevisiae: Great treatment experience without pain or regular medication</h3>
+ <p>We selected the muscone gas molecule as the upstream control signal for the therapeutic system. We utilized the muscone receptor MOR215-1 from mouse olfactory epithelial cells and introduced it into a plasmid system expressed in Saccharomyces cerevisiae. Subsequently, we chose the mating pathway in Saccharomyces cerevisiae as the signal transduction pathway for the muscone signal within the yeast. Based on the corresponding literature, we optimized and replaced five amino acids at the C-terminus of the Gα protein in the original mating pathway, enabling the muscone receptor to be integrated into the mating pathway of Saccharomyces cerevisiae. Ultimately, this allows for the expression of downstream genes through the pFUS1 promoter. This composite part can be utilized in future iGEM design for brand-new smell-induced treatment.</p>
+ <div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
+ <img src="https://static.igem.wiki/teams/5187/wiki-contribution-fig/2.jpg" alt="ibd_figure" class="shadowed-image" style="width: 90%; max-width: 800px;">
+ <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 2 Muscone Molecular Switch in Saccharomyces Cerevisiae</p>
</div>
+ </div>
</div>
-
+
<div class="row mt-4">
<div class="col-lg-12">
- <h2 id="topic3">
- <h2>Topic3</h2>
- <hr>
- <p>Admission to Tsinghua for both undergraduate and graduate schools is extremely competitive. Undergraduate admissions for domestic students is decided through the gaokao, the Chinese national college entrance exam, which allows students to list Tsinghua University among their preferred college choices. While selectivity varies by province, the sheer number of high school students applying for college each year has resulted in overall acceptance rates far lower than 0.1% of all test takers. Admission to Tsinghua's graduate schools is also very competitive. Only about 16% of MBA applicants are admitted each year.</p>
- <p>Department of Mathematical Sciences
- The Department of Mathematical Sciences (DMS) was established in 1927.
- In 1952, Tsinghua DMS was merged with the Peking University Department of Mathematical Sciences. Then in 1979 it was renamed "Department of Applied Mathematics", and renamed again in 1999 to its current title.
- Tsinghua DMS has three institutes at present, the institute of Pure Mathematics which has 27 faculty members, the Institute of Applied Mathematics and Probability and Statistics which has 27 faculty members, and the Institute of Computational Mathematics and Operations Research which has 20 faculty members. There are currently about 400 undergraduate students and 200 graduate students.</p>
+ <h3>Tetrathionate Sensing Colonization System: High efficiency in treatment</h3>
+ <p>We selected the signaling pathway of the sensor, designed by a previous team, that can detect tetrathionate (a marker of IBD) and we connected the ALS3 adhesion protein downstream. When the system detects the marker of IBD, Saccharomyces cerevisiae can adhere to the intestinal tract by expressing the ALS3 protein, achieving the colonization of the engineered strain. Future iGEM may refer our design when considering colonization in guts for other diseases.</p>
+ <div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
+ <img src="https://static.igem.wiki/teams/5187/wiki-contribution-fig/3.jpg" alt="ibd_figure" class="shadowed-image" style="width: 90%; max-width: 800px;">
+ <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 3 Tetrathionate Sensing Colonization System</p>
+ </div>
</div>
</div>
-
+
<div class="row mt-4">
<div class="col-lg-12">
- <h2 id="topic4">
- <h2>Topic4</h2>
- <hr>
- <p>Tsinghua University is consistently ranked among the top universities in the Asia-Pacific according to major international university rankings. Tsinghua University ranked No. 1 in China, the whole of Asia-Oceania region and emerging countries according to the Times Higher Education, with its industry income, research, and teaching performance indicator placed at 1st, 4th and 9th respectively in the world. Internationally, Tsinghua was regarded as the most reputable Chinese university by the Times Higher Education World Reputation Rankings where, it has ranked 8th globally and 1st in the Asia-Pacific.</p>
- <p>The Engineering Research Center for Navigation Technology is a relatively young institute in the Department of Precision Instrument which was established in 2000, with the intention to "[pursue] excellence in the research and development in the field of high-accuracy inertial instruments and navigation technology, as well as in MEMS inertial sensor fields, and to provide advanced training for future scientists and engineers in the field of inertial technology." Its research interests cover high-accuracy inertial instruments and navigation technology, MEMS inertial sensors and systems, and precise electro-mechanical control systems and their application. As of 2012, the area of the center is 2900 square meters, including approximately 550 square meters of clean rooms. Equipment and instruments in this center are worth over 50 million RMB (US$7.56 million).</p>
- </div>
- <div class="image-container">
- <img src="https://static.igem.wiki/teams/5187/figure/ibd-figure.jpg" alt="ibd_figure" class="shadowed-image">
+ <h2 id="Treatment for IBD">
+ <h2>Treatment for IBD</h2>
+ <hr>
+ <p>As we mentioned in Parts section, we utilize innovative pathways in Yeast, in order to secrete lactic acid in focuses of IBD. We are confident this treatment can not only contribute to future iGEM teams or iGEM community but also IBD patient community.</p>
+ <p>Current IBD treatments typically involve surgery or drug therapy. However, surgery does not address the root cause of the disease and may lead to numerous complications. Existing drug therapies, primarily anti-inflammatory drugs and immune system suppressors, can help alleviate IBD but carry risks, including an increased likelihood of cancer or immune system disorders within the intestinal tract.</p>
+ <p>Due to these concerns, Tsinghua iGEM 2024 introduces lactic acid into IBD treatment. Lactic acid secreted by lactic acid bacterial has been shown to limit autoimmunity and alleviate inflammatory conditions in the gut. As lactic acid is naturally present in the human gut and secreted by probiotics, using it as a treatment offers minimal side effects while providing significant relief for IBD symptoms.</p>
+ <div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
+ <img src="https://static.igem.wiki/teams/5187/wiki-contribution-fig/4.jpg" alt="ibd_figure" class="shadowed-image" style="width: 90%; max-width: 800px;">
+ <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 4 Lactic Acid Treatment and Effects</p>
+ </div>
+ <p>In our project we combined the innovative Muscone molecular switch with lactic acid secretion pathway in yeast, with the combination, patients will no longer to take regular medication. All they need to do is turn on the Muscone Generator after having taken the Genetically modified yeast. And with our colonization system, the treatment will be high efficient and also have minimal influence on other healthy parts in gut</p>
+ <p>We hope this method can make a real contribution to the IBD patient community in the future, as we know they are currently burdened by painful treatments. For future iGEM teams focusing on IBD, we also hope our approach provides valuable insight and inspires the development of even better treatments.</p>
+ </div>
</div>
+
+ <div class="row mt-4">
+ <div class="col-lg-12">
+ <h3>References</h3>
+ <p>[1] Chen, Y., Gao, H., Zhao, J., Ross, R.P., Stanton, C., Zhang, H., Chen, W., & Yang, B. (2023). Exploiting lactic acid bacteria for inflammatory bowel disease: A recent update. Trends in Food Science & Technology.</p>
+ <p>[2] Wu, Xin et al. (2024). AAV-delivered muscone-induced transgene system for treating chronic diseases in mice via inhalation. Nature communications.</p>
+ </div>
</div>
+
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