From 939c8e29827199cbddd20bbaf06937de5216c82b Mon Sep 17 00:00:00 2001
From: HouTeng Chan <ht-chen21@mails.tsinghua.edu.cn>
Date: Tue, 1 Oct 2024 14:53:42 +0000
Subject: [PATCH] Update file proof-of-concept.html
---
wiki/pages/proof-of-concept.html | 15 +++++++++++++--
1 file changed, 13 insertions(+), 2 deletions(-)
diff --git a/wiki/pages/proof-of-concept.html b/wiki/pages/proof-of-concept.html
index 3d9c068c..39ed624d 100644
--- a/wiki/pages/proof-of-concept.html
+++ b/wiki/pages/proof-of-concept.html
@@ -84,10 +84,21 @@
<p>For more information, please refer to <a href="https://2024.igem.wiki/Tsinghua/description" style="color: #FF5151" >Description</a>.</p>
<h4>Experimental results</h4>
<div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
- <img src="https://static.igem.wiki/teams/5187/wiki-engineer-fig/fig1.png" alt="ibd_figure" class="shadowed-image" style="width: 40%; max-width: 400px;">
+ <img src="https://static.igem.wiki/teams/5187/wiki-engineer-fig/fig2.png" alt="ibd_figure" class="shadowed-image" style="width: 40%; max-width: 400px;">
<p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 2 Galactose-induced D-lactic acid secretion system</p>
</div>
- </div>
+ <p>We introduced a lactate dehydrogenase gene expressed by the galactose promoter into Saccharomyces cerevisiae and measured the content of D-lactic acid in the supernatant after induction. This validated that by introducing lactate dehydrogenase, we can alter the anaerobic metabolic pathway of S. cerevisiae to synthesize D-lactic acid, and the produced D-lactic acid can be secreted into the surrounding environment of the yeast.</p>
+ <p>For more information, please refer to <a href="https://2024.igem.wiki/Tsinghua/description" style="color: #FF5151" >Description</a>.</p>
+ <p>Shortcoming: Experimental findings have shown that the nutritional composition of the yeast culture environment has a significant impact on the secretion of lactic acid; In the anaerobic environment, yeast can still produce alcohol, which may have an impact on the health of patients.</p>
+ <p>Solution:By further modifying the yeast's anaerobic metabolic pathways through genomic engineering, the impact of the nutritional composition of the culture environment on lactic acid secretion can be reduced; knockout of the alcohol dehydrogenase gene in the yeast genome.</p>
+ <h3>Proof of whole system<h3>
+ <h4>Experimental results</h4>
+ <div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
+ <img src="https://static.igem.wiki/teams/5187/wiki-engineer-fig/fig3.png" alt="ibd_figure" class="shadowed-image" style="width: 40%; max-width: 400px;">
+ <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 3 Muscone-induced lactate measurement results of the treatment system. (gal: induced by galactose; glc: induced by glucose; mus: induced by muscone)</p>
+ </div>
+ <p>We have simultaneously introduced the muscone molecular switch and the downstream lactate dehydrogenase into Saccharomyces cerevisiae, constructing a complete therapeutic system within the organism. After induction with muscone, we tested the content of D-lactic acid in the culture supernatant. The experimental results demonstrate that our complete therapeutic system can achieve specific secretion of lactic acid. For further discussion on the experimental results, please refer to <a href="https://2024.igem.wiki/tsinghua/therapy-system" style="color: #FF5151" >Therapy system</a></p>
+ </div>
</div>
--
GitLab