From 39494583379e759b555bd9b91d9262e7d151c010 Mon Sep 17 00:00:00 2001
From: HouTeng Chan <ht-chen21@mails.tsinghua.edu.cn>
Date: Tue, 24 Sep 2024 14:55:49 +0000
Subject: [PATCH] Update file engineering.html
---
wiki/pages/engineering.html | 8 ++++----
1 file changed, 4 insertions(+), 4 deletions(-)
diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html
index 4aa104cc..ab0bcc0c 100644
--- a/wiki/pages/engineering.html
+++ b/wiki/pages/engineering.html
@@ -138,13 +138,13 @@
<p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 7 STE2 gRNA&Cas9-pML107 plasmid</p>
</div>
<h5>Test</h5>
- <p>According to the new experimental design, we conducted the induction experiment again, and the results are as follows. It can be found that this time, the transformed yeast induced by glucose only has a very low level of lactate secretion.</p>
+ <p>We performed plasmid double transformation on the mating pathway knockout yeast strain and wild-type yeast (control), and then conducted the muscone induction experiment again. The results are as follows. We found that compared with the wild type, the nonspecific fluorescence signal was significantly weakened in the yeast strain with mating pathway knockout after induction with glucose.</p>
<div class="image-container" style="display: flex; flex-direction: column; align-items: center;">
- <img src="https://static.igem.wiki/teams/5187/wiki-engineering-fig/figure3.png" alt="ibd_figure" class="shadowed-image" style="width: 30%; max-width: 350px;">
- <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 3 Corrected lactate secretion induction experiment (wt: wild-type yeast; ldhA: transformed ldhA plasmid yeast; gal: induced by galactose; glc: induced by glucose)</p>
+ <img src="https://static.igem.wiki/teams/5187/wiki-engineering-fig/figure8.png" alt="ibd_figure" class="shadowed-image" style="width: 30%; max-width: 350px;">
+ <p style="text-align: center; font-size: 0.9em; margin-top: 10px;">fig 8 Corrected muscone induction experiment (Ko2: selected one of the CRISPR knockout strains; Gal: induced by galactose; Glc: induced by glucose; +: induced by muscone; -: no special treatment)</p>
</div>
<h5>Learn</h5>
- <p>Fortunately, the leakage expression of the ldhA gene is very low, and the induction of the galactose promoter is also effective. It is only because the difference in carbon sources will affect the reproduction and glycolytic metabolism rate of yeast itself, which in turn affects the overall lactate secretion level. However, these do not affect our project. Later, the promoter of the ldhA gene will be changed to the pFUS1 promoter regulated by the downstream of the yeast mating pathway, and the promoter of the receptor will also use the constitutive promoter in yeast.</p>
+ <p>Although the experimental results are in line with our expectations, unfortunately, knocking out the mating pathway in yeast itself cannot completely remove the nonspecific noise. Moreover, what is more confusing is that the specific signal of the group induced by galactose is reduced after gene knockout. We tried to explain and solve this problem, but due to the time limit of the iGEM competition, we did not achieve good results. For more details, please see wet lab. </p>
</div>
</div>
--
GitLab