diff --git a/src/contents/Human Practices/Conclisuin.tsx b/src/contents/Human Practices/Conclisuin.tsx index f51aa1c3c686a1ea24feed624664db51267c6b0e..2850189ca591979e53fb9a31eea57c2c58d504cf 100644 --- a/src/contents/Human Practices/Conclisuin.tsx +++ b/src/contents/Human Practices/Conclisuin.tsx @@ -220,12 +220,7 @@ function AnalyseSriram(){ <img src="https://static.igem.wiki/teams/5247/photos/sriram-interview.webp" alt="" /> </figure> </div> - <div className="col"> - <figure> - <img src="//TODO (ISI)" alt=""/> - - </figure> - </div> + </div> <p><strong>Contamination challenge:</strong> Helped us address a major issue with fungal contamination in our CF cell cultures, which were unstable and prone to infection.</p> @@ -284,12 +279,7 @@ function AnalyseIgnatova(){ </figure> </div> - <div className="col"> - <figure> - <img src="//TODO (ISI)" alt=""/> - - </figure> - </div> + </div> <p><strong>Introduction through iGEM Hamburg:</strong> Collaborated with Prof. Ignatova, a leading expert in CF research, to deepen our understanding of the disease.</p> @@ -320,12 +310,7 @@ function AnalyseWischmeyer(){ <figcaption>Figure. Patch-clamp measurement.</figcaption> </figure> </div> - <div className="col"> - <figure> - <img src="//TODO (ISI)" alt=""/> - - </figure> - </div> + </div> <p><strong>Support from Dr. Oliver Dräger:</strong> Hands-on support from Dr. Dräger helped us perform patch-clamp experiments using HEK cells provided by Uni Leuven.</p> @@ -360,12 +345,7 @@ function AnalyseMattijs(){ </figcaption> </figure> </div> - <div className="col"> - <figure> - <img src="//TODO (ISI)" alt=""/> - - </figure> - </div> + </div> <p><strong>Consultation because of test system:</strong> HEK cells with corresponding CFTR mutation derived from Leuven were successfully cultured in our lab for testing purposes.</p> @@ -502,12 +482,7 @@ function AnalyseMoor(){ <img src="https://static.igem.wiki/teams/5247/integrated-human-practices/chitosan.webp" alt="" /> </figure> </div> - <div className="col"> - <figure> - <img src="//TODO (ISI)" alt=""/> - - </figure> - </div> + </div> <p><strong>Chitosan properties:</strong> Provided valuable insights into chitosan, a cationic polymer with strong potential to stabilize RNA in lipid nanoparticle (LNP) formulations due to its robust protection against RNases and heat stability.</p> diff --git a/src/contents/results.tsx b/src/contents/results.tsx index 3eb47f6042591dc7c4c10acea23aa27a7fd9541d..1154938e873a82b0088b9f4c21b0b04456f02fee 100644 --- a/src/contents/results.tsx +++ b/src/contents/results.tsx @@ -220,12 +220,12 @@ To further enhance the stability and sustainability of the LNPs for inhalation, <p>24 h, 48 h and 72 h post-transfection, we observed in the conditions with Lipofectamine alone, or combined with DNA or RNA, no fluorescence, indicating unsuccessful transfection. Similarly, no fluorescence was seen in cells treated with LNPs alone or in combination with DNA or RNA. When LNPs were combined with Minicircle DNA, clear fluorescence was observed, indicating successful transfection and expression of our eYFP reporter under this condition (figure X). However, a strong background fluorescence from the OptiMEM medium was observed, complicating the analysis.</p> <p>Overall, among all the tested conditions, the LNP formulation with Minicircle DNA was the only combination that resulted in noticeable fluorescence, suggesting it to be the most effective transfection method for HEK293 cells in this experiment.</p> <figure> - <img src="Insert URL here"/> + <img src="https://static.igem.wiki/teams/5247/delivery/results/precyse/cayman.png"/> <figcaption> - <b>Figure X. </b> - Figure X Fluorescence microscopic images of transfected HEK293 cells at 20x magnification after 72 h post-transfection with different Cayman LNP formulations recorded with Leica DMI6000 B. + <b>Figure 18. </b> + Overlay of phase contrast and fluorescence microscopic images of transfected HEK293 cells at 20x magnification after 72 h post-transfection with different Cayman LNP formulations recorded with Leica DMI6000 B. For Lipofectamine (Lipo) + Minicircle DNA (Mini DNA) only the fluorescence image is shown. </figcaption> - </figure> + <H5 text="SEM"/> <div className="row align-items-center"> <div className="col">