diff --git a/wiki/pages/description.html b/wiki/pages/description.html
index ac3d34370ad42d29390b048d0a09c0c050697d92..066f32dce8086d1b7b9792914ec64220ed4a1e11 100644
--- a/wiki/pages/description.html
+++ b/wiki/pages/description.html
@@ -143,9 +143,8 @@
<div class="row mt-4">
<div class="col-lg-12">
- <h2>Our Project Presentation Video</h2>
+ <h2>Watch Our Presentation Video</h2>
<hr>
- <p>UZurich: RhyzUp - Drought Resistance Through Biofilm Enhancement (2024) - Team Presentation [English]</p>
<iframe width="560" height="315" src="https://video.igem.org/videos/embed/b2a999ad-14d2-4a4e-b0cb-b9fc67681cf5" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe>
</div>
</div>
diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html
index 0e01fc4d85ea8846287510be67f6305dae64c65c..5084bdecec7876e9abd1aa9defef1273fca9149d 100644
--- a/wiki/pages/engineering.html
+++ b/wiki/pages/engineering.html
@@ -49,7 +49,7 @@
We researched all the gene sequences, attached the appropriate adaptors and ordered them through Twist.
</p>
<div class="image-model">
- <img src="https://static.igem.wiki/teams/5250/project/engineering/picture-1-updated-min.png" alt="Construct" class="img-fluid" style="width: 90%;display: block; margin-left: auto; margin-right: auto;">
+ <img src="https://static.igem.wiki/teams/5250/project/engineering/picture-1-updated-min.png" alt="Construct" class="img-fluid responsive-img" style="display: block; margin-left: auto; margin-right: auto;">
<p style="padding-left:30px; padding-right:30px;">
<b>Figure 1:</b> This illustration represents our full construct to enhance biofilm formation only when the bacteria are in proximity to plant roots. Created with BioRender.com
</p>
@@ -174,7 +174,7 @@
The newly assembled plasmid was transformed into <i>E. coli</i> SY327 and then introduced into <i>P. sp.</i> IsoF using Triparental Conjugation.
</p>
<div class="row mt-4 image-description">
- <img src="https://static.igem.wiki/teams/5250/project/engineering/picture-2-pm-testing-sensing-plasmid-pt12rhyz01-1.png" alt="plasmid map testing sensing plasmid" class="img-fluid " style="width: 50%; display: block; margin-left: auto; margin-right: auto;">
+ <img src="https://static.igem.wiki/teams/5250/project/engineering/picture-2-pm-testing-sensing-plasmid-pt12rhyz01-1.png" alt="plasmid map testing sensing plasmid" class="img-fluid responsive-img-smaller" style="display: block; margin-left: auto; margin-right: auto;">
<p style="text-align:center;">
<b>Figure 3:</b> Plasmid map of p12Rhyz01
</p>
@@ -185,7 +185,8 @@
<div class="row mt-4">
<h4>Test</h4>
<p>
- Unfortunately the oligo annealing and digestion ligation failed, due to unspecific binding of the primer. For future experiments we will therefore repeat this step and then conduct the second sensing assay.
+ Unfortunately the oligo annealing and digestion ligation failed, due to unspecific binding of the primer.
+ For future experiments we will therefore repeat this step and then conduct the second sensing assay.
</p>
</div>
@@ -292,22 +293,22 @@
<tr>
<td style="border: 1px solid #ddd; padding: 8px;">DGC PisoF R196A</td>
<td style="border: 1px solid #ddd; padding: 8px;"><i>Pseudomonas sp.</i> IsoF</td>
- <td style="border: 1px solid #ddd; padding: 8px;">arginine at position 196 was altered to an alanine </td>
+ <td style="border: 1px solid #ddd; padding: 8px;">arginine at position 196 was substituted with an alanine </td>
</tr>
<tr>
<td style="border: 1px solid #ddd; padding: 8px;">DGC PisoF R240A</td>
- <td style="border: 1px solid #ddd; padding: 8px;"><i>P. sp.</i> IsoF</td>
- <td style="border: 1px solid #ddd; padding: 8px;">arginine at position 240 was altered to an alanine</td>
+ <td style="border: 1px solid #ddd; padding: 8px;"><i>Pseudomonas sp.</i> IsoF</td>
+ <td style="border: 1px solid #ddd; padding: 8px;">arginine at position 240 was substituted with an alanine</td>
</tr>
<tr>
<td style="border: 1px solid #ddd; padding: 8px;">DGC WspR wt</td>
- <td style="border: 1px solid #ddd; padding: 8px;"><i>P. aeruginosa</i></td>
+ <td style="border: 1px solid #ddd; padding: 8px;"><i>Pseudomonas aeruginosa</i></td>
<td style="border: 1px solid #ddd; padding: 8px;">none</td>
</tr>
<tr>
<td style="border: 1px solid #ddd; padding: 8px;">DGC WspR R242A</td>
- <td style="border: 1px solid #ddd; padding: 8px;"><i>P. aeruginosa</i></td>
- <td style="border: 1px solid #ddd; padding: 8px;">arginine at position 242 was altered to an alanine</td>
+ <td style="border: 1px solid #ddd; padding: 8px;"><i>Pseudomonas aeruginosa</i></td>
+ <td style="border: 1px solid #ddd; padding: 8px;">arginine at position 242 was substituted with an alanine</td>
</tr>
</table>
<div class="image-description" style="padding-top:0; padding-left:0">
@@ -700,8 +701,8 @@
<hr>
<h3 style="color:black; font-size:20px;">Design and Build</h3>
<p>
- To ensure that the bacteria die when they move away from the plant roots, we incorporated a kill switch into our construct using a gyrase-inhibiting Toxin/Antitoxin system CcdB/CcdA.
- We selected the CcdB/CcdA system. The ccdB gene encodes the toxic protein CcdB, which inhibits the DNA gyrase and therefore leads to cell death.
+ To ensure that the bacteria die when they move away from the plant roots, we incorporated a kill switch into our construct using the gyrase-inhibiting Toxin/Antitoxin system CcdB/CcdA..
+ The ccdB gene encodes the toxic protein CcdB, which inhibits the DNA gyrase and therefore leads to cell death.
The ccdA gene encodes the antitoxin protein CcdA that neutralizes the toxin by forming a CcdA-CcdB complex and therefore protecting the cell<a href="#ref10">10</a>.
</p>
<p>
@@ -844,14 +845,14 @@
<div class="row mt-4">
<h3 style="color:black; font-size:20px;">Learn</h3>
<p>
- All of our assays, conducted both in various <i>E. coli</i> strains and <i>P. sp.</i> IsoF, were unsuccessful.
- Bacterial growth was not inhibited by the toxin.
- Since we used <i>E. coli</i> strains that are not resistant to the CcdB toxin, we suspect that our promoter is too weak, resulting in insufficient toxin production unable to affect bacterial survival.
- Our findings indicate that a high intracellular concentration of the toxin is necessary to inhibit growth.
- It seems unlikely that the toxin effectively targets the gyrase of <i>P. sp.</i> IsoF.
- However, given that the toxin is well-documented and its efficiency is established but still did not inhibit growth in our <i>E. coli</i> strains, we are unable to draw definitive conclusions about the effect of CcdB on <i>P. sp.</i> IsoF.
- The lack of growth inhibition in our experiments suggests an issue with the expression of the toxin or insufficient toxin levels.
- Since the toxin wasn’t strong enough to kill the bacteria, there was no reason to test whether the antitoxin could neutralize the toxin.
+ For <i>E. coli</i> CSH50 we see a significant decrease in growth when inducing it with more rhamnose.
+ This is not the case for <i>P. sp.</i> IsoF, the CFU remains consistent across all tested rhamnose concentrations.
+ We can thus conclude that the toxin CcdB does inhibit the growth of <i>E. coli</i> CSH50 in this second toxin assay but it does not inhibit the growth of <i>P. sp.</i> IsoF.
+ We don’t know why the first toxin test didn’t lead to the same result as the second toxin test for <i>E. coli</i> strains.
+ Since our aim is to implement this Kill Switch into <i>P. sp.</i> IsoF, our main focus was on determining whether this toxin was effective in <i>P. sp.</i> IsoF.
+ For this bacterial species, the result is clear.
+ In both experiments there is no inhibition in growth for <i>P. sp.</i> IsoF. We can thus conclude that the toxin CcdB doesn’t effect the growth of <i>P. sp.</i> IsoF significantly.
+ For the future, we would therefore research more on toxin antitoxin systems specifically designed for <i>P. sp.</i> IsoF and implement one of those into our genetic circuits.
</p>
</div>
@@ -944,7 +945,7 @@
<li>
We successfully mutated the DGC PisoF_00565, removing its negative regulation through c-di-GMP.
Our mutated DGC PisoF_00565 R196A resulted in higher c-di-GMP concentration compared to the wildtype sequence. This indicates that we successfully modified the enzyme’s I-site, which usually reduces enzyme activity when c-di-GMP binds. By altering the sequence of the DGC we prevented c-di-GMP from binding to the negative allosteric site, which effectively enhanced the enzyme’s activity.
- This has never been done and showed before for any DGC native to <i>P. sp. IsoF</i>.
+ This has never been done and showed before for any DGC native to <i>P. sp. </i>IsoF.
</li>
</ol>
<br>
diff --git a/wiki/pages/home.html b/wiki/pages/home.html
index a62912758557f91b227858100bd7e8b049ff98eb..a43658f534fc620db708554adea468db2d2af7a5 100644
--- a/wiki/pages/home.html
+++ b/wiki/pages/home.html
@@ -188,10 +188,9 @@
</div>
</div>
<div class="row mt-4">
- <div class="col-lg-12">
+ <div class="col-lg-12" style="text-align: center;">
<h2>The (Almost) Original Tale of How Our Project Idea Was Born</h2>
<hr>
- <p>UZurich: RhyzUp - Drought Resistance Through Biofilm (2024) - Project Promotion [English]</p>
<iframe width="560" height="315" src="https://video.igem.org/videos/embed/664b10eb-142f-406e-8832-5ce95e465e2c" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe>
</div>
</div>
diff --git a/wiki/pages/parts.html b/wiki/pages/parts.html
index 2d1fcee1db4e6ff0f6a3cae66928568b8930b0d2..d1641d935ea936714ab82b96ff86dc56c3de4552 100644
--- a/wiki/pages/parts.html
+++ b/wiki/pages/parts.html
@@ -392,8 +392,8 @@
</p>
<p>
<ol>
- <li>pT7-like + PisoF_00565 DGC R242A, Pxut + T7-like RNAP, pT7-like + T7-like RNAP, pT7-like + PDE-inhibiting sgRNA</li>
- <li>pT7-like + PisoF_00565 DGC R242A, constitutive promoter + XutR, pT7-like + toxin, Pxut + antitoxin</li>
+ <li>pT7-like + DGC WspR R242A, Pxut + T7-like RNAP, pT7-like + T7-like RNAP, pT7-like + PDE-inhibiting sgRNA</li>
+ <li>pT7-like + DGC WspR R242A, constitutive promoter + XutR, pT7-like + toxin, Pxut + antitoxin</li>
</ol>
</p>