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When Your Vision Changes,
Your Life Will Change.

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Glaucoma-specific

miRNAs

in the patient's

Tear Fluid

are sequence-specifically
recognized by the

TWJ-Complex.

SDA Reaction

Strand Displacement Amplification

Next,
Polymerase and Nickase
repeatedly cleave and
elongate the miRNA to
generate ssDNA
one after another.

CRISPR-Cas3

Then,
CRISPR-Cas3 recognizes the
amplified product,
cleaving the
FITC-ssDNA-Biotin reporter.

LFA

Lateral Flow Assay

If FITC-ssDNA-Biotin is cleaved,
T-line appears in the strip,
and if it is remained,
no T-line appears.

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