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<h2 id='what-kinds-of-situations-are-lantern-similar-devices-suitable-for'>What kinds of situations are
LANTERN-similar devices suitable for?</h2>
- <p>Synthetic biology is an engineering discipline — there is a desire to build things that do not yet
- exist (Church et al., 2014). We reflect on the essence in the realm of "engineering", seeking
- modular methods for gene circuit synthesis. Drawing inspiration from digital circuits, we completed the design of
- LANTERN, a programmable logic framework. </p>
<p>Through LANTERN, we can generate 16 different output mappings from two input molecules. In LANTERN we use IPTG and
rha as input small molecules and GFP fluorescence as the output signal. This represents a new paradigm—— elements
like LANTERN should be able to evolve to accept any molecule as input and produce any signal as output. Based on
@@ -205,10 +201,6 @@
style="zoom: 60%;">
<div style="color: #666666; padding: 2px;">Fig 3. five phases divided by two intermediate metabolites</div>
</center>
- <p>LANTERN's simple display also gives it the potential for everyday applications, such as home food fermentation,
- like making pickles. </p>
- <p>we can use Lantern to program the detection of any harmful substances, thereby achieving the effect of simply
- detecting harmful substances.</p>
<h3 id='synthetic-circuits-integrating-logic'>Synthetic circuits integrating logic</h3>
<p>Since the rise of synthetic biology, scientists have been searching for a strategy for efficiently assembling
synthetic genetic circuits to achieve Boolean logic functions with stable DNA-encoded memory of events(Siuti et al.,
@@ -218,10 +210,10 @@
biological memory storage systems.</p>
<h4 id='synthetic-biology-system-stability-regulation-module'>Synthetic Biology System Stability Regulation Module
</h4>
- <p>We hope that LANTERN-similar devices can contribute to the synthetic biology community. We soon realized that they
+ <p>We hope that LANTERN-like devices can contribute to the synthetic biology community. We soon realized that they
could play a role in preventing mutations and detecting the normal operation of genetic pathways.</p>
- <p>As an example, we can look at the UCAS-China 2023 project "NOX: Neo-quOrum sensing-based Xpression biosensor
- platform." NOX provides a highly compatible and robust platform with impressive performance. Chimeric receptors
+ <p>Take the UCAS-China 2023 project "NOX: Neo-quOrum sensing-based Xpression biosensor platform." as an
+ example." NOX provides a highly compatible and robust platform with impressive performance. Chimeric receptors
are assembled for optimal compatibility, and the orthogonal quorum sensing module is responsible for luminescence,
optimized through modeling (see: <a
href='https://2023.igem.wiki/ucas-china/'>https://2023.igem.wiki/ucas-china/</a>). In the experimental design, NOX
@@ -236,9 +228,10 @@
<h2 id='drylab'>Drylab</h2>
<h3 id='software'>Software</h3>
<p>To support our project's pre-research phase and give visualization about how our circuits work, we developed two
- tools: (1) a circuit visualization website , illustrating the functionality of our genetic circuits and making the
+ tools: (1) a circuit visualization website, illustrating the functionality of our genetic circuits and making the
programmable process of LANTERN easier; (2) Ask Lantern, a natural language processing model for searching Biobricks
- (See Software for more details), aimed at reducing the pre-research time finding suitable Biobricks from the massive
+ (See <a href='https://2024.igem.wiki/ucas-china/software'>Software</a> for more details), aimed at reducing the
+ pre-research time finding suitable Biobricks from the massive
Biobricks database.</p>
<p>For project pre-research, we developed a natural language query program for the Biobricks database called Ask
Lantern (See <a href='https://2024.igem.wiki/ucas-china/software'>Software</a> for more details), aimed at reducing
@@ -255,8 +248,7 @@
<p>Our model comprises four components: 1) ensuring functionality of constant promoter, 2) characteristics of the
logic AND Gate, 3) Demonstrating the inhibition induced by CRISPRi, and 4) estimating the time consumption of the
boolean
- logic circuit system. Modeling is employed to predict outcomes and support the verification of LANTERN, as
- illustrated in the four components shown in the figure.</p>
+ logic circuit system.</p>
<center>
<img src="https://static.igem.wiki/teams/5276/model/abstractnew2.webp" alt="orthogonality-design-1-new.webp"
style="zoom: 60%;">
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<div>
<p>
- <b>Programmable Logic Devices (PLDs)</b> are engineering chips that can be manually reconfigured after
+ <b>Programmable Logic Devices (PLDs)</b> are chips that can be manually reconfigured after
manufacturing, enabling them to perform a variety of logic functions. Inspired by PLDs, we have designed a
programmable synthetic biology framework, LANTERN, which allows users to customize logic gates tailored to
- their specific scenarios.
+ specific scenarios.
</p>
</div>
</div>
@@ -362,26 +362,19 @@
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<div class="pages-flexbox-vertical">
- <p>
- With the help of recombinases and CRISPRi, LANTERN can <b>switch between different types of logic gates based on
- user requirements</b>. <b>Recombinases</b> are used as tools to reprogram the circuits, while <b>CRISPRi
- functions</b>
- as a NOT gate. By simply adjusting the inputs and outputs according to specific needs, LANTERN can be
- reconfigured into virtually any desired logic gate.
- </p>
+ <p><strong>Recombinases</strong> are used as tools to reprogram the circuits, while <strong>CRISPRi
+ functions</strong> as a NOT gate. LANTERN can be then reconfigured into virtually any desired logic gate by
+ simply adjusting the inputs and outputs according to specific needs.</p>
</div>
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<div class="blankroom"></div>
<div class="pages-multicol">
<div class="pages-flexbox-vertical">
- <p>
- LANTERN introduces the concept of <b>"programmable pathway synthetic biology"</b>, addressing the need to construct
- and customize various logical relationships in the lab with potential applications in industrial
- manufacturing. Drawing inspiration from PLDs in electrical engineering, LANTERN bridges the gap between
- engineering and bioengineering. <b>This represents what we want to contribute to the iGEM community —
- harnessing engineering principles to uncover the mysteries of biology.</b>
- </p>
+ <p>LANTERN introduces the concept of "programmable pathways," addressing the need to construct and
+ customize various logical relationships in the lab with potential applications in industrial manufacturing.
+ Drawing inspiration from PLDs in electrical engineering, this also represents the ideology we want to convey
+ to the iGEM community — <em>harnessing engineering principles to uncover the mysteries of biology</em>.</p>
</div>
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@@ -395,11 +388,9 @@
<p id="lantern-p">
Why the name <b>LANTERN</b>?
</p>
- <p class="animate-on-scroll-heading enter">
- The digits "0" and "1" are fundamental to logic gates, and we noticed that their shapes can perfectly form the
- pattern of a traditional Chinese lantern. This inspired us to name our system LANTERN, symbolizing both its
- foundation in logic and its connection to cultural heritage.
- </p>
+ <p>The digits "0" and "1" are fundamental to logic gates, and we noticed that their shapes
+ can perfectly form the pattern of a traditional Chinese lantern. This inspired us to name our system LANTERN,
+ symbolizing both its foundation in logic and its connection to cultural heritage.</p>
</div>
<div class="pages-flexbox-vertical">
<img src="https://static.igem.wiki/teams/5276/wiki/pages/homepage/whitelogo.png" alt="placeholder" id="p2image">