From 14f221f2b6d63e0da8a337caedd5d2b30eaf1379 Mon Sep 17 00:00:00 2001
From: Mara Valverde <maravalverde2@gmail.com>
Date: Tue, 26 Nov 2024 10:38:06 +0000
Subject: [PATCH] Update scaling.md
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docs/scaling.md | 30 ++----------------------------
1 file changed, 2 insertions(+), 28 deletions(-)
diff --git a/docs/scaling.md b/docs/scaling.md
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# 📈 Scale Up
-<div id="scalable_page" style={{display:"flex", flexDirection:"row", justifyContent:"space-between"}}>
+Previous small-scale experiments using <i>E. coli</i> BL21-Gold (DE3) engineered with a plasmid containing a bacteriophage lysis gene and a Tardigrade-derived stability-enhancing gene showed promising lysate activity. However, the flask setups resulted in lower titers, which required us to produce several batches, which had different activities. After our talks with Dr. AnÃbal Arce and Dr. Fernando Guzmán, larger batches should enable us to achieve higher and more consistent yields, which are crucial for the characterization of our lysate and ensure reproducibility.
-<div style={{flex: "0 0 100%"}}>
-<p>
- Previous small-scale experiments using <i>E. coli</i> BL21-Gold (DE3) engineered with a plasmid containing a bacteriophage lysis gene and a Tardigrade-derived stability-enhancing gene showed promising lysate activity. However, the flask setups faced challenges related to aeration and mixing, resulting in lower yields. Transitioning to bioreactors offers improved control over these parameters, enabling us to simulate industrial production conditions and achieve higher, more consistent yields, which are crucial for optimizing the process and scaling up production.
-</p>
-
-<div class="section">
- <details style={{ width: '45%' }}>
- <summary>
- <h3>Problem</h3>
- <h4>Low lysate yield from flask fermentation</h4>
- </summary>
- <p></p>
- </details>
-
- <details style={{ width: '45%' }}>
- <summary>
- <h3>Solution</h3>
- <h4>Performing fermentation in bioreactors</h4>
- </summary>
- <p></p>
- </details>
-</div>
-
-<!-- <hr/> -->
-
-<p>
- Based on results from shake flasks and plate reader experiments, <i>E. coli</i> BL21-Gold (DE3) strain transformed with a plasmid encoding lysis <i>gene R</i> from bacteriophage and Gene 1 <i>(CAHS107838)</i> from Tardigrades, was providing the most active and stable lysate in our experiments. This strain was chosen to be used for bioreactor runs.
+We used <i>E. coli</i> BL21-Gold (DE3) transformed with a plasmid encoding lysis <i>gene R</i> from bacteriophage lambda and Gene 1 <i>(CAHS107838)</i> for our upscaling experiments.
</p>
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