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 {% extends "layout.html" %}
   
 {% block title %}Contribution{% endblock %}
-{% block lead %}Make a useful contribution for future iGEM teams. Use this page to document that contribution.{% endblock %}
+{% block lead %}{% endblock %}
 
 {% block page_content %}
 
 <h1>NitroBLAST as a foundation for novel nitrogen fixation research</h1>
 
-<p><p>
+<p>Transplanting a nitroplast from its host into other cells is a gigantic undertaking, but one that has similarly massive potential for breakthrough advancements in engineering living organisms. We aim to start a line of research aimed at this very goal, and to this end have 
+left behind registered parts, drylab resources and software as well as our combined knowledge and understanding of the nitroplast and its relation to its host.
+</p>
 
 
-<h2>Background</h2>
+<h2>Characterization of the UCYN-A transit peptide</h2>
 
-<p>The Netherlands has been facing a pressing <strong>nitrogen crisis</strong> for several years. This crisis is largely attributed to the <strong>agriculture sector</strong>, with over 80% of ammonia (a nitrogenous compound) emissions coming from manure <a href="#cite1">[1]</a> and chemical fertilizers <a href="#cite2">[2]</a>. The over-use of fertilizers has a detrimental effect on the environment through the deposition of excess nitrogen oxides and ammonia in the ground, excessively enriching the environment with nutrients promoting uncontrolled plant and algal growth, or eutrophication, a form of nutrient imbalance <a href="#cite3">[3]</a> that negatively impacts the local biodiversity. This highlights the need of the hour: a solution for <strong>increasing global food supply while maintaining environmental standards</strong>.</p>
+<p>From proteome data released this year, we were able to elucidate motifs corresponding to the sequence of a transit peptide localizing proteins to UCYN-A. This protein, submitted as a part, is crucial for any future experiment aiming to 
+deliver proteins to UCYN-A, whether in its native host to investigate its behavior or within a foreign recipient cell, when trying to keep a transplanted nitroplast alive.
+</p>
 
-<h2>Nitrogen Crisis in the Netherlands </h2>
+<h2>New assembly of <i>Braadurosphera bigelowii</i>'s transcriptome and proteome prediction</h2>
 
+<p>note to self ask balint</p>
+
+<h2> Isolation of UCYN-A from <i>Braadurosphera bigelowii</i> and transplantation</h2>
+
+<p>We have established a protocol for UCYN-A insertion onto animal and plant cells through PEG fusion.</p>
+
+<h2>Model and roadmap</h2>
+
+<p>The team has identified, through research and correspondence with experts, the core requirements needed in the future for a viable transplant of a nitroplast into foreign cells; These steps can be broken up in self-contained modules to be tackled individually, and when each of them is achieved nitrogen-fixing eukaryotes will truly be on the horizon. 
+In addition, we have created a model to quantify the nitrogen-fixation capability of a hypothetical nitroplast recipient, based on the involved metabolic networks.
+</p>
 
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