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index af530a60a6e7688a623434af7d7890d4029c0cbb..81e9c32deb84075f6108c8bceb8257588cc8b0f4 100644
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                   <div class="h3">Materials and Reagents</div>
                   <ul style="text-align:justify; font-family:AccidenzCommons; color:#185A4F; font-weight:400; font-size: min(1.5vw, 22px); font-style: normal; line-height: normal;">
                   <li>YPD (yeast extract peptone dextrose) liquid media</li>
-                  </ul>
+                  <li>Hâ‚‚O (sterile)</li>
+                    <li>Polyethylene glycol 3350 (PEG 3350; 50% w/v)</li>
+                    <li>TE buffer (pH 8.0)</li>
+                    <li>Single-stranded carrier DNA (2mg/ml)</li>
+                    <li>1.0M Lithium acetate stock solution (LiAc)</li>
+                    <li>Plasmid DNA and yeast cells</li>
+                    </ul>
                   <div class="h3">Equipment</div>
                   <ul style="text-align:justify; font-family:AccidenzCommons; color:#185A4F; font-weight:400; font-size: min(1.5vw, 22px); font-style: normal; line-height: normal;">
-                    <li></li>
-                  </ul>
+                  <li>Spectrophotometer</li>
+                      <li>Water baths (30°C, 42°C, 96°C)</li>
+                      <li>Shaking incubator</li>
+                      <li>Centrifuge</li>
+                      <li>Vortex mixer</li>
+                      <li>Micropipettes and sterile tips</li>
+                      <li>Microfuge tubes and 50ml centrifuge tubes</li>
+                    </ul>
+                    
                   <div class="h3">Procedure</div>
                   <ol style="text-align:justify; font-family:AccidenzCommons; color:#185A4F; font-weight:400; font-size: min(1.5vw, 22px); font-style: normal; line-height: normal;">
-                    <li></li>
-                  </ol>
+                   <li>Inoculate yeast cells in 100ml YPD and grow overnight at 30°C, 200rpm.</li>
+                      <li>After 12-16h, measure culture density:</li>
+                      <ul>
+                        <li>Dilute 10μl cells in 1ml water</li>
+                        <li>Measure OD₆₆₀ (0.1 OD₆₆₀ = 1x10⁶ cells/ml)</li>
+                      </ul>
+                      <li>Add 2.5x10⁸ cells to 50ml pre-warmed YPD (final concentration: 5x10⁶ cells/ml).</li>
+                      <li>Incubate at 30°C, 200rpm until density reaches 2x10⁷ cells/ml (~4 hours).</li>
+                      <li>Harvest cells: centrifuge at 3000g for 5 minutes.</li>
+                      <li>Wash cells with 25ml sterile Hâ‚‚O, and centrifuge again.</li>
+                      <li>Resuspend cell pellet in 1.0ml of 0.1M LiAc, transfer to 1.5ml microfuge tube.</li>
+                      <li>Centrifuge at top speed for 5 seconds, and remove LiAc.</li>
+                      <li>Resuspend cells in 0.1M LiAc to a final volume of 500μl (about 400μl LiAc).</li>
+                      <li>Prepare carrier DNA: boil 1.0ml sample for 5 minutes, then chill on ice.</li>
+                      <li>Aliquot 50μl of cell suspension into labeled microfuge tubes, centrifuge, and remove LiAc.</li>
+                      <li>Add transformation mix in this order:</li>
+                      <ul>
+                        <li>240μl PEG 3350 (50% w/v)</li>
+                        <li>36μl 1.0M LiAc</li>
+                        <li>25μl single-stranded carrier DNA (2.0mg/ml)</li>
+                        <li>50μl H₂O + plasmid DNA (0.1-10μg)</li>
+                      </ul>
+                      <li>Vortex vigorously until the cell pellet is completely mixed (~1 minute).</li>
+                      <li>Incubate 30 minutes at 30°C.</li>
+                      <li>Heat shock for 30 minutes at 42°C.</li>
+                      <li>Centrifuge at 6000-8000rpm for 15s, remove transformation mix.</li>
+                      <li>Resuspend pellet in 0.2ml sterile Hâ‚‚O.</li>
+                      <li>Plate cells in different concentrations on selective plates. Recommended:</li>
+                      <ul>
+                        <li>10μl cells + 100μl H₂O</li>
+                        <li>200μl cells undiluted</li>
+                      </ul>
+                      <li>Incubate plates at 30°C for 3 days.</li>
+                    </ol>
+                    
              <div class="h2"><i>C. reinhardtii </i> Transformation</div>
                   <div class="h3">Materials and Reagents</div>
                   <ul style="text-align:justify; font-family:AccidenzCommons; color:#185A4F; font-weight:400; font-size: min(1.5vw, 22px); font-style: normal; line-height: normal;">