From cfef6cc293f1e0049913b74efb53c1dfc79cb6b7 Mon Sep 17 00:00:00 2001
From: Yuhao Lin <lyh2001213@163.com>
Date: Wed, 2 Oct 2024 05:14:01 +0000
Subject: [PATCH] =?UTF-8?q?=E4=BF=AE=E6=94=B9LB=20medium=20preparation?=
=?UTF-8?q?=E5=85=B7=E4=BD=93=E6=AD=A5=E9=AA=A4?=
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---
src/contents/experiments.tsx | 50 +++++++++++++++++++++++++++++++++++-
1 file changed, 49 insertions(+), 1 deletion(-)
diff --git a/src/contents/experiments.tsx b/src/contents/experiments.tsx
index ae2df44..ff5cbcc 100644
--- a/src/contents/experiments.tsx
+++ b/src/contents/experiments.tsx
@@ -169,7 +169,55 @@ export function Experiments() {
<p>Content for section 2.</p>
<div className="rounded-border">
<h4 className="center-text">LB medium preparation</h4>
- <p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p>
+ <p className="indent">(1) Material
+LB Broth Agar Powder, Miller
+LB Broth Powder, Miller
+Ultrapure (UP) Water
+Double Distillation Water (ddH2O)
+Ampicillin
+Kanamycin
+Chloramphenicol
+
+(2) Steps
+LB liquid medium
+1. Weigh 12.5g of LB Broth Powder.
+2. Add the powder into 500ml UP Water.
+3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
+
+LB solid medium
+1. Weigh 12g of LB Broth Agar Powder.
+2. Add the powder into 300ml UP Water.
+3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
+4. Pour the medium into culture dishes in the ultraclean worktable.
+
+LB selective medium (Ampicillin)
+1. Weigh 12g of LB Broth Agar Powder.
+2. Add the powder into 300ml UP Water.
+3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
+4. Weigh 1g of Ampicillin.
+5. Add the ampicillin into ddH2O and volume to 10mL. The ddH2O is sterilized previously. Shake the mixture fully.
+6. Filter the mixture through a filter to remove microorganism.
+7. Add 500μL of mixture into an EP tube with 500μL ddH2O. The ddH2O is sterilized previously.
+8. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes.
+
+LB selective medium (Kanamycin)
+1. Weigh 12g of LB Broth Agar Powder.
+2. Add the powder into 300ml UP Water.
+3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
+4. Weigh 100mg of Kanamycin.
+5. Add the kanamycin into ddH2O and volume to 10mL. The ddH2O is sterilized previously. Shake the mixture fully.
+6. Filter the mixture through a filter to remove microorganism.
+7. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes.
+
+LB selective medium (Chloramphenicol)
+1. Weigh 12g of LB Broth Agar Powder.
+2. Add the powder into 300ml UP Water.
+3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
+4. Weigh 500mg of Chloramphenicol.
+5. Add the ampicillin into anhydrous ethanol and volume to 10mL. Shake the mixture fully.
+6. Filter the mixture through a filter to remove microorganism.
+7. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes.
+</p>
<p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p>
</div>
</Element>
--
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