diff --git a/src/contents/experiments.tsx b/src/contents/experiments.tsx index ae2df4467dea56b499726e1b1f5a3f574a2ca5c4..ff5cbcc5f4cf6e2c0c6facd72a919dee5e6ac74f 100644 --- a/src/contents/experiments.tsx +++ b/src/contents/experiments.tsx @@ -169,7 +169,55 @@ export function Experiments() { <p>Content for section 2.</p> <div className="rounded-border"> <h4 className="center-text">LB medium preparation</h4> - <p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p> + <p className="indent">(1) Material +LB Broth Agar Powder, Miller +LB Broth Powder, Miller +Ultrapure (UP) Water +Double Distillation Water (ddH2O) +Ampicillin +Kanamycin +Chloramphenicol + +(2) Steps +LB liquid medium +1. Weigh 12.5g of LB Broth Powder. +2. Add the powder into 500ml UP Water. +3. Autoclave entire bottle of LB medium under 121°C for 20 minutes. + +LB solid medium +1. Weigh 12g of LB Broth Agar Powder. +2. Add the powder into 300ml UP Water. +3. Autoclave entire bottle of LB medium under 121°C for 20 minutes. +4. Pour the medium into culture dishes in the ultraclean worktable. + +LB selective medium (Ampicillin) +1. Weigh 12g of LB Broth Agar Powder. +2. Add the powder into 300ml UP Water. +3. Autoclave entire bottle of LB medium under 121°C for 20 minutes. +4. Weigh 1g of Ampicillin. +5. Add the ampicillin into ddH2O and volume to 10mL. The ddH2O is sterilized previously. Shake the mixture fully. +6. Filter the mixture through a filter to remove microorganism. +7. Add 500μL of mixture into an EP tube with 500μL ddH2O. The ddH2O is sterilized previously. +8. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes. + +LB selective medium (Kanamycin) +1. Weigh 12g of LB Broth Agar Powder. +2. Add the powder into 300ml UP Water. +3. Autoclave entire bottle of LB medium under 121°C for 20 minutes. +4. Weigh 100mg of Kanamycin. +5. Add the kanamycin into ddH2O and volume to 10mL. The ddH2O is sterilized previously. Shake the mixture fully. +6. Filter the mixture through a filter to remove microorganism. +7. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes. + +LB selective medium (Chloramphenicol) +1. Weigh 12g of LB Broth Agar Powder. +2. Add the powder into 300ml UP Water. +3. Autoclave entire bottle of LB medium under 121°C for 20 minutes. +4. Weigh 500mg of Chloramphenicol. +5. Add the ampicillin into anhydrous ethanol and volume to 10mL. Shake the mixture fully. +6. Filter the mixture through a filter to remove microorganism. +7. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes. +</p> <p className="indent">las ijffs aiskfd fskj iiwls asd.aass ffas awssd awus iisal fask.aisisad ksjdfkaf iwjasifjakdshf wijdfalksjf wiksjkfjksalhf, gsahfjhgejkfh uhaejkfh sjdihgfuqiw jh sjiafhjsaj fh asd.</p> </div> </Element>