diff --git a/static/style.css b/static/style.css
index 8b59fb7ff4f996f5815826d06c518c06bc488a14..3f0567aa9575c289f41c21c1795e7c5a298c483f 100644
--- a/static/style.css
+++ b/static/style.css
@@ -67,4 +67,61 @@ footer a:hover { color: white; text-decoration: underline; }
.lab-info {
display: inline-block;
margin: auto;
-}
\ No newline at end of file
+}
+
+.rounded-green {
+ border-radius: 25px;
+ padding-top: 20px;
+ padding-left: 20px;
+ padding-right: 20px;
+ padding-bottom: 20px;
+
+ width: 25%;
+ height: fit-content;
+ display: block;
+ margin: auto;
+
+ background-color: #45b06cff;
+
+ p, h2 {
+ text-align: center;
+ }
+}
+
+.rounded-blue {
+ border-radius: 25px;
+ padding-top: 20px;
+ padding-left: 20px;
+ padding-right: 20px;
+ padding-bottom: 20px;
+
+ width: 25%;
+ height: fit-content;
+ display: block;
+ margin: auto;
+
+ background-color: rgb(91, 126, 177);
+
+ p, h2 {
+ text-align: center;
+ }
+}
+
+.rounded-red {
+ border-radius: 25px;
+ padding-top: 20px;
+ padding-left: 20px;
+ padding-right: 20px;
+ padding-bottom: 20px;
+
+ width: 50%;
+ height: fit-content;
+ display: block;
+ margin: auto;
+
+ background-color: rgb(179, 86, 86);
+
+ p, h2 {
+ text-align: center;
+ }
+}
diff --git a/wiki/pages/safety.html b/wiki/pages/safety.html
index 47b52cdca366acb56cf30abc683e621ecd8c73c8..78ce7979be03e8daf4647e8f8e00339b444081c3 100644
--- a/wiki/pages/safety.html
+++ b/wiki/pages/safety.html
@@ -7,43 +7,123 @@
<div class="row mt-4">
<div class="col">
- <div class="bd-callout bd-callout-info">
- <h4>Safety and Security Award</h4>
- <p>Synthetic biology will need to be used safely and securely if local people are to solve local problems all around the world. The Safety and Security Committee is challenging teams to apply biological engineering approaches to manage risks associated with synthetic biology. Can you take the next step in incremental progress towards knowledge, understanding, and tools that will make the use of synthetic biology safer and more secure?</p>
- <p>To compete for the Safety and Security award, please describe your work on this page and also fill out the description on the <a href="https://competition.igem.org/deliverables/judging-form">judging form</a>.</p>
+ <h2>Overview</h2>
+ <hr>
+ <p>In the lab, we will compare DNA damage and repair efficiency in wild-type versus
+ genetically modified strains of E. coli, each exposed to equal amounts of ionizing radiation (mimicking UV radiation conditions in space) in a controlled setting. If safe irradiation is not achievable, we may mimic UV radiation-induced DNA damage by other methods to cause cyclobutane pyrimidine dimers. We will use synthetic biology to engineer a plasmid to act as a vector for specific DNA repair genes. Certain tests and sequencing will be necessary to ensure success of transformation before we detect DNA damage by observing the viability of the bacteria after exposure to UV radiation.
+ </div>
+</div>
+
+<div class="row">
+ <div class="row mt-4 ">
+ <div class="col-lg-4 rounded-green">
+ <h2>Hygiene</h2>
+ <hr>
+ <ul>
+ <li>No food or drinks in lab</li>
+ <li>Wash Hands</li>
+ <li>Wash equipment after lab</li>
+ </ul>
+ </div>
+ <div class="col-lg-4 rounded-blue">
+ <h2>PPE</h2>
+ <hr>
+ <ul>
+ <li>Long Pants only</li>
+ <li>Gloves at all times</li>
+ <li>Lab Coats as Needed</li>
+ <li>Closed-toe Shoes</li>
+ <li>Long Hair Tied Back</li>
+ </ul>
+ </div>
+ </div>
+
+ <div class="row mt-4">
+ <div class="col-lg-4 rounded-red">
+ <h2>Biohazard Disposal</h2>
+ <hr>
+ <p>Use proper biohazard disposal containers and processes</p>
+ </div>
+ </div>
+
+ <div class="row mt-4">
+ <div class="col-lg-4 rounded-blue">
+ <h2>Storage</h2>
+ <hr>
+ <ul>
+ <li>Proper labeling for all reagents</li>
+ <li>Proper hazard labeling and convention for all reagents</li>
+ <li>Proper understanding and use of storage units</li>
+ </ul>
+ </div>
+ <div class="col-lg-4 rounded-green">
+ <h2>Handling</h2>
+ <hr>
+ <ul>
+ <li>Prior research on operating lab equipment</li>
+ <li>Proper pipetting procedures</li>
+ <li>Research and rehearsal before each planned procedure</li>
+ </ul>
+ </div>
+ </div>
+
+ <div class="row mt-4">
+ <div class="col-lg-4 rounded-red">
+ <h2>Emergency Procedures</h2>
<hr>
- <p>Please see the <a href="https://competition.igem.org/judging/awards">2024 Awards Page</a> for more information.</p>
+ <ul>
+ <li>Checked Fire Safety Routes/Procedures</li>
+ <li>Emergency Aid Kit location and use</li>
+ <li>Emergency eyewash and shower location and use</li>
+ </ul>
</div>
</div>
</div>
+
<div class="row mt-4">
<div class="col">
- <h2>What should this page contain?</h2>
+ <h2>Lab Safety</h2>
<hr>
- <p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
- <div class="bd-callout bd-callout-info">
- <p>Please visit the <a href="https://responsibility.igem.org/safety-policies/introduction">Safety Policies page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
- </div>
+ <p>
+ For the wet lab portion of our project, we worked in the Love Lab at SDSU. On the first few days of our lab, we got trained on how to use the scientific equipment safely.
+ </p>
+ <p>
+ We were supervised by Dr. Love and a few graduate students, including Sierra Murrell, a Ph.D. student, at all times. We were comfortable asking questions whenever we were unsure about a protocol, and we made sure to take precautions when working with equipment we were less familiar with as a group, such as the Nanodrop.
+ </p>
</div>
</div>
<div class="row mt-4">
- <div class="col-lg-8">
- <h2>Safe Project Design</h2>
+ <div class="col">
+ <h2>Possible Risks</h2>
<hr>
- <p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
- <ul>
- <li>Choosing a non-pathogenic chassis</li>
- <li>Choosing parts that will not harm humans / animals / plants</li>
- <li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
- <li>Including an "induced lethality" or "kill-switch" device</li>
- </ul>
- </div>
- <div class="col-lg-4">
- <h2>Safe Lab Work</h2>
+ <p>
+ Getting exposed to UV radiation has a high risk to humans. While we were operating gel recovery, we always wore face covers and long-sleeve lab coats so that we could minimize the exposure to UV radiation. People who did not use the Gel Transilluminators closed their eyes while one person was working on the machine. When we were using the bunsen burners we had to make sure no gas was leaking out after we used them. We were careful to avoid holding our hands or arms over the flame.
+ </p>
+ </div>
+</div>
+
+<div class="row mt-4">
+ <div class="col">
+ <h2>Modifications</h2>
+ <hr>
+ <p>
+ Instead of using ethidium bromide, a minor carcinogen, for dying our gels, we used SYBR Safe to avoid unnecessary risks. Ethidium bromide is known to potentially cause irritation on exposure [1]. We also decided to use E. coli rather than other types of bacteria to not only simplify our lab but to also ensure that our lab stayed at biosafety level 1. We used TOP10 cells for our initial transformation, which is an organism that falls under biosafety level 1 [2].
+ </p>
+ </div>
+</div>
+
+<div class="row mt-4">
+ <div class="col">
+ <h2>References</h2>
<hr>
- <p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+ <p>
+ [1] Environmental Health and Safety, University of Iowa. "Ethidium Bromide." Environmental Health and Safety, <a src="www.ehs.research.uiowa.edu/ethidium-bromide"></a>. Accessed 17 Sept. 2024.
+ </p>
+ <p>
+ [2] IBA Lifesciences. "Competent E. coli TOP10." IBA Lifesciences, <a src="www.iba-lifesciences.com/Competent-E.-coli-TOP10/5-1600-020"></a>. Accessed 17 Sept. 2024.
+ </p>
</div>
</div>