From 809a907e17472ab71cbf41ae90f7e73642fe6256 Mon Sep 17 00:00:00 2001
From: dawn gamberale <dgamberale@powayusd.com>
Date: Wed, 19 Jun 2024 23:01:58 +0000
Subject: [PATCH] Update description.html
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wiki/pages/description.html | 2 +-
1 file changed, 1 insertion(+), 1 deletion(-)
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<div class="row mt-4">
<div class="col-lg-8">
- <h2>The Problem of UV Radiation</h2>
+ <h2>Our Approach</h2>
<hr>
<p> We plan to determine what genes are most efficient for initiating DNA repair in humans. These would include genes that code proteins involved in DNA-repair complexes, and genes that code transcription factors for such complexes. There are certain existing organisms that have evolved strong resistance to DNA damage by UV radiation, and we will be specifically investigating the molecular basis of these defense mechanisms. One DNA repair mechanism that we will be investigating and potentially exploiting is Nucleotide Excision Repair, especially that of Global Genome Repair (known as GG-NER). This is a highly conserved mechanism that is common in all three domains of life, and it has been found in both humans and bacteria such as E. Coli. Once we have determined the specific necessary proteins and their gene sequences, we test their effectiveness in bacteria in the lab. </p>
<p> In the lab, we will compare DNA damage and repair efficiency in wild-type versus genetically modified strains of E. Coli exposed to equal amounts of UV radiation (mimicking the conditions in space). We will use synthetic biology to bioengineer a plasmid to act as a vector for DNA repair-inducing genes. Certain tests will be necessary to ensure bacteria viability and success of transformation before we use COMET assays to detect DNA damage. </p>
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