From f23033c08f9f00bc41d8796e3e5fda79be87fb12 Mon Sep 17 00:00:00 2001
From: tbliki <timofej.bliki@ru.nl>
Date: Wed, 7 Aug 2024 19:39:45 +0200
Subject: [PATCH] Almost done with protocols! - Attached all known protocols -
Have to contact other team members to get information about undone protocols
- Left as highlighted yellow for unsure ones for now To do: - Upload images
---
wiki/pages/notebook.html | 70 +++++++++++++++++++++++++++++-----------
1 file changed, 51 insertions(+), 19 deletions(-)
diff --git a/wiki/pages/notebook.html b/wiki/pages/notebook.html
index 4f59e8a..8b9f919 100644
--- a/wiki/pages/notebook.html
+++ b/wiki/pages/notebook.html
@@ -210,7 +210,6 @@
<!-- Notebook page display -->
<div>
<div class="backdrop" id="backdrop"> </div>
-
<div class="viewbox" id="viewbox" style="border: 2px solid black; background-color: #ff424bff;">
<div style="background-color: #ff424bff; color: white; flex: 1; padding:5px;">
<h1 id="task" style="text-align: center;">Task</h1>
@@ -333,7 +332,6 @@
</ul>
<div id="PCR-Amplification-journal">
- <!-- Insert the actual lab notes here. -->
<table>
<tr><td class="journal-td">
<p>16th of May 2024</p>
@@ -346,7 +344,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>Link to PCR Protocol(??)</li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/pcr-procedure-radboud-university-team.pdf" target="_blank">
+ Factor 8 Plasmid Amplification
+ </a></li>
</ul>
<h4>Experimental:</h4>
<p>
@@ -368,7 +368,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>Link to agarose gel electrophoresis(??)</li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/denaturing-agarose-gel-electrophoresis.pdf" target="_blank">
+ Native Agarose Gel Electrophoresis of RNA
+ </a></li>
</ul>
<h4>Experimental:</h4>
<p>
@@ -394,7 +396,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>Link to gel extraction protocol(??)</li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/qiagen-gel-extraction-protocol.pdf" target="_blank">
+ QIAGEN Gel Extraction Protocol
+ </a></li>
</ul>
<h4>Experimental:</h4>
<p>
@@ -406,23 +410,19 @@
</div>
<div id="page-Plasmid-Amplification" class="retrievable">
<p id="Plasmid-Amplification-task">
- <!-- Insert the name of the notebook page page riight there. -->
Plasmid Amplification of FVIII
</p>
<p id="Plasmid-Amplification-datetime">
- <!-- Insert the time(s) below this comment-->
03/07/24 - 08/07/24
</p>
<ul id="Plasmid-Amplification-participants">
- <!-- Insert each participant within its own <li></li> tag below.-->
<li>Kate</li>
<li>Lea</li>
</ul>
<div id="Plasmid-Amplification-journal">
- <!-- Insert the actual lab notes here. -->
<table>
<tr><td class="journal-td">
<p>3rd of July 2024 - 4th of July 2024</p>
@@ -433,7 +433,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>link to plasmid cloning protocol</li>
+ <li><p style="background-color:yellow">
+ Link to plasmid cloning protocol (?) <!-- Insert Protocol -->
+ </p></li>
</ul>
<h4>Experimental:</h4>
<p>
@@ -452,7 +454,15 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>link to miniprep protocol, enzyme digestion and gel electrophoresis protocol</li>
+ <li><p style="background-color:yellow">
+ link to miniprep protocol (??) <!-- Insert Protocol -->
+ </p></li>
+ <li><p style="background-color:yellow">
+ link to enzyme digestion protocol (??) <!-- Insert Protocol -->
+ </p></li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/denaturing-agarose-gel-electrophoresis.pdf" target="_blank">
+ Native Agarose Gel Electrophoresis of RNA
+ </a></li>
</ul>
<h4>Experimental:</h4>
<p>
@@ -483,7 +493,12 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>link to enzyme digestion and DNA purification column protocol</li>
+ <li><p style="background-color:yellow">
+ link to enzyme digestion protocol (??)
+ </p></li>
+ <li><p style="background-color:yellow">
+ link to DNA purification column protocol (??)
+ </p></li>
</ul>
<h4>Experimental:</h4>
<p>
@@ -520,7 +535,6 @@
</ul>
<div id="In-vitro-journal">
- <!-- Insert the actual lab notes here. -->
<table>
<tr><td class="journal-td">
<p>9th of July 2024</p>
@@ -531,7 +545,12 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>link to IVT protocol and agarose gel electrophoresis protocol</li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/in-vitro-synthesis-of-rna-using-t7-rna-polymerase.pdf">
+ <i>in vitro</i> synthesis of RNA using T7 RNA polymerase
+ </a></li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/denaturing-agarose-gel-electrophoresis.pdf">
+ Native Agarose Gel Electrophoresis of RNA
+ </a></li>
</ul>
<h4>Experimental:</h4>
<p>
@@ -561,7 +580,12 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>link to IVT, gel electrophoresis</li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/in-vitro-synthesis-of-rna-using-t7-rna-polymerase.pdf">
+ <i>in vitro</i> synthesis of RNA using T7 RNA polymerase
+ </a></li>
+ <li><a href="https://static.igem.wiki/teams/5342/documents/protocols/denaturing-agarose-gel-electrophoresis.pdf">
+ Native Agarose Gel Electrophoresis of RNA
+ </a></li>
</ul>
<h4>Experimental:</h4>
<p>Overview:</p>
@@ -635,7 +659,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>Protocol based on...</li>
+ <li><p style="background-color:yellow">
+ Protocol based on <a href="https://www.protocols.io/view/rna-clean-up-by-phenol-chloroform-yxmvmw46g3pe/v2?step=null">this</a> (??) <!-- Insert Protocol -->
+ </p></li>
</ul>
<h4>Experimental:</h4>
<ol>
@@ -670,7 +696,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>?</li>
+ <li><p style="background-color:yellow">
+ No protocol or same protocol (??) <!-- Insert Protocol -->
+ </p></li>
</ul>
<h4>Experimental:</h4>
<ol>
@@ -694,7 +722,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>?</li>
+ <li><p style="background-color:yellow">
+ No protocol (??) <!-- Insert Protocol -->
+ </p></li>
</ul>
<h4>Experimental:</h4>
<ol>
@@ -729,7 +759,9 @@
</ul>
<h4>Protocols:</h4>
<ul>
- <li>?</li>
+ <li><p style="background-color:yellow">
+ No protocol (??) <!-- Insert Protocol -->
+ </p></li>
</ul>
<h4>Experimental:</h4>
<ol>
--
GitLab