From d071e4dde1c71b25d562b445681f3fc4361b45b1 Mon Sep 17 00:00:00 2001
From: anvin-f <anvinfu@gmail.com>
Date: Wed, 2 Oct 2024 18:17:56 +0800
Subject: [PATCH] w
---
static/styles/style.css | 7 ++++--
wiki/menu.html | 4 ++--
wiki/pages/engineering.html | 45 ++++++++++++++++++++++++++++++++-----
wiki/pages/results.html | 38 ++++++++++++++++++++++++++++++-
4 files changed, 84 insertions(+), 10 deletions(-)
diff --git a/static/styles/style.css b/static/styles/style.css
index 8233458..f03e624 100644
--- a/static/styles/style.css
+++ b/static/styles/style.css
@@ -370,9 +370,12 @@ td {
width: 100%;
overflow: scroll;
top: 8vh;
- background-image: url(https://static.igem.wiki/teams/5441/pictures/bg-2.png); background-size: 100%;
- }
+ }
+ .enginesidebar .col-lg-12{
+ background-image: url(https://static.igem.wiki/teams/5441/pictures/bg-2.png); background-size: 100%;
+ margin-bottom:10px;
+ }
}
.eduBar{
diff --git a/wiki/menu.html b/wiki/menu.html
index 2e0e887..fa95f6f 100644
--- a/wiki/menu.html
+++ b/wiki/menu.html
@@ -36,8 +36,8 @@
</li>
<li><a class="nav-link" href="{{ url_for('pages', page = 'safety') }}">Safety</a></li>
<li><a class="nav-link" href="{{ url_for('pages', page = 'human-practices') }}">Human Practices</a></li>
- <li><a class="nav-link" href="{{ url_for('pages', page = 'education') }}">Education</a></li>
- <li><a class="nav-link" href="{{ url_for('pages', page = 'awards') }}">Awards</a></li>
+ <li><a class="nav-link" href="{{ url_for('pages', page = 'education') }}">Education</a></li>-->
+ <!--<li><a class="nav-link" href="{{ url_for('pages', page = 'awards') }}">Awards</a></li>
<!--<li>
<button class="nav-link dropdown-btn" data-dropdown="dropdown3" aria-expanded="false">
Award
diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html
index 5bf577c..7a107f3 100644
--- a/wiki/pages/engineering.html
+++ b/wiki/pages/engineering.html
@@ -46,7 +46,14 @@ endblock %}
position: relative;
top: -2%;
left: 30%;
- " href="#test1"></a></div>
+ " href="#test1"></a><a style="
+ width: 55%;
+ aspect-ratio: 2/1.3;
+ display: block;
+ position: absolute;
+ top: 0;
+ left: 20%;
+ " href="#cycle1"></a></div>
</div>
<div class="col-lg-12">
<div style="
@@ -83,7 +90,14 @@ endblock %}
position: relative;
top: -2%;
left: 30%;
- " href="#test2"></a></div>
+ " href="#test2"></a><a style="
+ width: 55%;
+ aspect-ratio: 2/1.3;
+ display: block;
+ position: absolute;
+ top: 0;
+ left: 20%;
+ " href="#cycle2"></a></div>
</div>
<div class="col-lg-12">
<div style="
@@ -120,7 +134,14 @@ endblock %}
position: relative;
top: -2%;
left: 30%;
- " href="#test3"></a></div>
+ " href="#test3"></a><a style="
+ width: 55%;
+ aspect-ratio: 2/1.3;
+ display: block;
+ position: absolute;
+ top: 0;
+ left: 20%;
+ " href="#cycle3"></a></div>
</div>
<div class="col-lg-12">
<div style="
@@ -157,7 +178,14 @@ endblock %}
position: relative;
top: -2%;
left: 30%;
- " href="#test4"></a></div>
+ " href="#test4"></a><a style="
+ width: 55%;
+ aspect-ratio: 2/1.3;
+ display: block;
+ position: absolute;
+ top: 0;
+ left: 20%;
+ " href="#cycle4"></a></div>
</div>
<div class="col-lg-12">
<div style="
@@ -194,7 +222,14 @@ endblock %}
position: relative;
top: -2%;
left: 30%;
- " href="#test5"></a></div>
+ " href="#test5"></a><a style="
+ width: 55%;
+ aspect-ratio: 2/1.3;
+ display: block;
+ position: absolute;
+ top: 0;
+ left: 20%;
+ " href="#cycle5"></a></div>
</div>
</div>
</div>
diff --git a/wiki/pages/results.html b/wiki/pages/results.html
index 6c92bec..3a1d330 100644
--- a/wiki/pages/results.html
+++ b/wiki/pages/results.html
@@ -160,7 +160,43 @@
<p><span style="font-weight: 400;">The </span><strong>results from the Gaussia Luciferase Flash Assay Kit</strong><span style="font-weight: 400;"> were collected via the use of a </span><strong>luminometer (multimode microplate reader)</strong><span style="font-weight: 400;">, choosing all wavelengths for luminescence. With the help of iGEM team HKUST 2024 & supervision under Dr. Yu from G.T. College. We were able to ascertain the results below.</span></p>
<p><span style="font-weight: 400;">Utilizing </span><strong>two-way anova</strong><span style="font-weight: 400;">, we studied whether the plasmid, pENTR1A-PSMA-Gluc (BBa_K5441012), concentration, cancer cell (MLLB-2) concentration or a combination of both can affect the luminescence signal. Our results showed </span><strong>individual cancer cell concentration (p < 0.001), individual plasmid concentration (p = 0.036 < 0.05) and the combination of both, plasmid*cancer cell has a p-value = 0.006 (p < 0.05)</strong><span style="font-weight: 400;">. This result contributes to the luminesce level.</span><strong> Our control setup does not contain any transfected plasmids</strong><span style="font-weight: 400;">.</span></p>
+<p><span style="font-weight: 400;">In Figure X,</span></p>
+<p> </p>
+<ul>
+<li style="font-weight: 400;"><span style="font-weight: 400;">The well without plasmids, no matter the amount of cancer cells, shows no significant differences in luminescence reading. This proves that our engineered plasmid does not induce any elevated nor reduced levels of luminescence. </span></li>
+</ul>
+<p> </p>
+<ul>
+<li style="font-weight: 400;"><span style="font-weight: 400;">In </span><strong>low plasmid concentration (0.000911 μg / μL), both high-concentration (100,000 cells per </strong><strong>0.33 mm</strong><strong>²) and low-concentration (10,000 cells </strong><strong>per 0.33 mm</strong><strong>²) display significant differences (p = 0.002, < 0.01)</strong><span style="font-weight: 400;">. This shows that high-concentration of cancer cells have a significantly lower luminescence value (12.95 RLU) than that of low-concentration cancer cells (29.94 RLU). </span></li>
+</ul>
+<p> </p>
+<ul>
+<li style="font-weight: 400;"><strong>In medium (0.00455</strong> <strong>μg / μL) and high (0.00909</strong> <strong>μg/μL) plasmid concentration</strong><span style="font-weight: 400;">,</span><strong> low-concentration cancer cells always have a significantly higher luminescence value than that of ones found in medium and high-concentration (p < 0.01)</strong><span style="font-weight: 400;">.</span></li>
+</ul>
+
+<p><span style="font-weight: 400;">In Figure Y, </span></p>
+<p> </p>
+<ul>
+<li style="font-weight: 400;"><span style="font-weight: 400;">A more obvious result can be seen. We found that a </span><strong>high concentration of plasmids combined with any cancer cell concentration leads to lower luminescence.</strong><span style="font-weight: 400;"> </span></li>
+</ul>
+<p> </p>
+<ul>
+<li style="font-weight: 400;"><span style="font-weight: 400;">In order to detect cancer cells effectively, one should employ the following ratio </span><br>(in 1 mL plasmid) = 4.55 μg:10,000 cancer cells; In 50,000 cancer cells, as recommended 0.911 μg</li>
+</ul>
+<p><strong>Conclusion and Prediction :</strong></p>
+<p> </p>
+<p><span style="font-weight: 400;">In general, </span><strong>low-level cancer cell concentration requires high-level plasmid concentration for signaling</strong><span style="font-weight: 400;">. On the contrary, high-level cancer cell concentration requires low-level plasmid concentration. </span></p>
+<p> </p>
+<p><span style="font-weight: 400;">However, </span><strong>lower levels of plasmid concentration used results in a lower level of signaling</strong><span style="font-weight: 400;">. When encountering a </span><strong>low concentration of cancer cells, we recommend using a high plasmid concentration</strong><span style="font-weight: 400;">. </span></p>
+<p> </p>
+<p><span style="font-weight: 400;">According to our graphs, the trend shows that when a</span><strong> lower cell concentration is paired with a medium concentration of plasmid</strong><span style="font-weight: 400;">, the </span><strong>luminescence value observed is higher</strong><span style="font-weight: 400;">.</span></p>
+<p> </p>
+<p><span style="font-weight: 400;">At</span><strong> high-concentration of cancer cells</strong><span style="font-weight: 400;">, it will produce the </span><strong>lowest luminescence signaling among all three concentrations of plasmids</strong><span style="font-weight: 400;"> we studied. It can be explained by high concentration of prostate cancer cells producing a larger amount of acidic metabolic waste at a higher rate, the more acidic culture medium of high concentration prostate cancer cells lowers the rate of their protein synthesis.</span></p>
+<p><br /><br /></p>
+<p><span style="font-weight: 400;">This graph can be used for further extrapolation upon the availability of more evidence.</span></p>
+<p> </p>
+<!--
<div class="row mt-4">
<img src = "https://static.igem.wiki/teams/5441/result/bar.png"class="col-lg-6">
<img src = "https://static.igem.wiki/teams/5441/result/line.png"class="col-lg-6">
@@ -211,7 +247,7 @@
<img src = "https://static.igem.wiki/teams/5441/result/bax1.png"class="col-lg-4">
<img src = "https://static.igem.wiki/teams/5441/result/bax2.png"class="col-lg-4">
<img src = "https://static.igem.wiki/teams/5441/result/bax3.png"class="col-lg-4">
-</div>
+</div>-->
<!--<p><span>Introduce low conc of bax,0.00124ug/ul, day 2, 3 no</span></p>-->
<!--<p><span>Insert: graph (Ivan)</span></p>-->
--
GitLab