From 43e672605b72cb0e1cdb0894b8f5709e2f6d75d0 Mon Sep 17 00:00:00 2001
From: anvin-f <anvinfu@gmail.com>
Date: Wed, 2 Oct 2024 09:42:13 +0800
Subject: [PATCH] notebook
---
wiki/pages/notebook.html | 465 +++++++++++++++++++--------------------
1 file changed, 222 insertions(+), 243 deletions(-)
diff --git a/wiki/pages/notebook.html b/wiki/pages/notebook.html
index 4c65fea..e24f915 100644
--- a/wiki/pages/notebook.html
+++ b/wiki/pages/notebook.html
@@ -29,13 +29,13 @@ day for your project.{% endblock %}
<a href="#Mar Week 3"><img src="https://static.igem.wiki/teams/5441/notebook/guides.png" style="width: min(420px, 79%); position: absolute; top: min(410px, 58.5%); left: min(75px, 10%)"></a>
</div>
</div>
- <h2>March</h2>
+
<div style="
background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
background-size: 100% 100%;
padding: 7%;
- position:relative
-"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2584-1.png" style="position:absolute; width: min(40%, 500px);left: 30%; top: max(-10%, -20px)">
+ padding-top:max(10%,40px);position:relative;margin-top:70px
+"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2584-1.png" style="position:absolute; width: min(40%, 300px);left: 30%; top: -20px">
<a name="Mar Week 1"></a>
<h5><span>Week 1: Inspiration from other teams</span></h5>
@@ -63,15 +63,13 @@ day for your project.{% endblock %}
</ul>
</div>
-
-
<div style="
- background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2583-1.png);
+ background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
background-size: 100% 100%;
padding: 7%;
- position:relative;
-">
- <img src="https://static.igem.wiki/teams/5441/notebook/img-2585-1.png" style="position:absolute; width: min(40%, 500px); left: 30%; top: max(-10%, -20px)">
+ padding-top:max(10%,40px);position:relative;margin-top:70px
+"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2585-1.png" style="position:absolute; width: min(40%, 300px);left: 30%; top: -20px">
+
<a name="Mar Week 2"></a>
<h5><span>Week 2: Delivery of plasmid to prostate</span></h5>
<ul>
@@ -100,215 +98,215 @@ day for your project.{% endblock %}
</ul>
</ul>
</div>
-
+
<div style="
- background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2581-1.png);
- background-size: 100% 100%;
- padding: 7%;
- position:relative;
-">
- <img src="https://static.igem.wiki/teams/5441/notebook/img-2589-1.png" style="position:absolute; width: min(40%, 500px); left: 30%; top: max(0%, -40px)">
- <a name="Mar Week 3"></a>
- <h5><span>Week 3: Guides of polymers - Aptamers</span></h5>
- <ul>
- <li><span>How to target prostate with the polymer? - Aptamers</span><span>1</span></li>
- <ul>
- <li><span>Sections of DNA/RNA/peptides that target specific molecules </span><span>3,4</span></li>
- <li><span>High specificity </span><span>5,6</span></li>
- <li><span>Produced in a batch → reduces production costs</span></li>
- <li><span>Reversible denaturation (unlike antibodies)</span></li>
- <li><span>Less immunogenic than antibodies</span></li>
- <li><span>Can be attached to nanoparticles/polymers</span></li>
- </ul>
- <li><span>Potential aptamer 1: EpDT3 </span><span></span><span>16</span></li>
- <ul>
- <li><span>Binds to EpCAM (epithelial cell adhesion molecule, overexpressed in some tumour cells) </span><span>17</span></li>
- <li><span>ðŸ‘ðŸ»EpCAM is highly expressed in metastasized PCa cells → detect later stages of cancer </span><span>19</span></li>
- <li><span>👎ðŸ»Still non-specific to PCa</span></li>
- </ul>
- <li><span>Dual aptamer design </span><span>23</span></li>
- <ul>
- <li><span>A10-3.2: PSMA +ve (but only 80% of PCa patients have PSMA)</span></li>
- <li><span>DUP-1: PSMA -ve</span></li>
- <li><span>Used in a lot of research previously</span></li>
- <li><span>We can add a constitutive promoter inside our plasmid to express the reporter genes.</span></li>
- </ul>
- </ul>
- <ul>
- <li><span>PD-L1 nanobody</span></li>
- <ul>
- <li><span>Normal cancer cells: have PD-L1 to “block†attacks from T-cells</span></li>
- <li><span>PD-L1 nanobody: binds to PD-L1 so that cancer cells have no way to block attacks</span></li>
- <li><span>Problem:</span></li>
- <ul>
- <li><span>requires immune cells to kill</span></li>
- <li><span>At later stages, immune system may be compromised</span></li>
- <li><span>Cannot kill cancer cells effectively</span></li>
- </ul>
- </ul>
- <li><span>Bax gene</span><span>(2./2.1)</span></li>
- <ul>
- <li><span>BCL2 associated X, apoptosis regulator</span></li>
- <li><span>If Bax protein is transcribed, it promotes apoptosis (type of programmed cell death) in cancer cells</span></li>
- </ul>
- <li><span>Bax has been chosen</span></li>
- </ul>
- </div>
- <div>
- <div style="position:relative;">
- <img src="https://static.igem.wiki/teams/5441/notebook/img-2521-1.png" style="width: 600px">
- <a href="#Apr Week 1"><img src="https://static.igem.wiki/teams/5441/notebook/img-2522-1.png" style="width: min(460px, 80%); position: absolute; top: min(255px, 28.5%); left: min(50px, 10%)"></a>
- <a href="#Apr Week 2"><img src="https://static.igem.wiki/teams/5441/notebook/img-2523-1.png" style="width: min(440px, 79%); position: absolute; top: min(330px, 36.5%); left: min(40px, 8%)"></a>
- <a href="#Apr Week 3"><img src="https://static.igem.wiki/teams/5441/notebook/img-2524-1.png" style="width: min(420px, 79%); position: absolute; top: min(380px, 49.5%); left: min(55px, 10%)"></a>
- <a href="#Apr Week 4"><img src="https://static.igem.wiki/teams/5441/notebook/img-2525-2.png" style="width: min(420px, 79%); position: absolute; top: min(480px, 57.5%); left: min(75px, 10%)"></a>
- </div>
- </div>
- <h2>April</h2>
- <div style="
- background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2409-1-1.png);
+ background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
background-size: 100% 100%;
padding: 7%;
- padding-top: min(90% ,250px);
-">
- <a name="Apr Week 1"></a>
- <h5><span>Week 1: Formulation of initial plan</span></h5>
- <ol>
- <li><span>Construction of plasmids</span></li>
- <li><span>Purchase of A and B separately</span></li>
- </ol>
- <ol>
- <li><span>A plasmid </span><strong>containing our gene</strong><span> that is </span><strong>non-mammalian</strong><span>; and</span></li>
- <li><span>Another plasmid that is </span><strong>mammalian </strong><span>but </span><strong>lacks any promoter genes</strong><span> (pENTR1A)</span></li>
- </ol>
- <ul>
- <li><span>A and B will be digested, then ligated together using </span><strong>BamHI </strong><span>and </span><strong>HindIII </strong><span>enzymes</span></li>
- <li><span>Colony PCR → confirm size of plasmids are correct 1.e</span></li>
+ padding-top:max(10%,40px);position:relative;margin-top:70px
+"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2589-1.png" style="position:absolute; width: min(40%, 300px);left: 30%; top: -20px">
+ <a name="Mar Week 3"></a>
+ <h5><span>Week 3: Guides of polymers - Aptamers</span></h5>
+ <ul>
+ <li><span>How to target prostate with the polymer? - Aptamers</span><span>1</span></li>
+ <ul>
+ <li><span>Sections of DNA/RNA/peptides that target specific molecules </span><span>3,4</span></li>
+ <li><span>High specificity </span><span>5,6</span></li>
+ <li><span>Produced in a batch → reduces production costs</span></li>
+ <li><span>Reversible denaturation (unlike antibodies)</span></li>
+ <li><span>Less immunogenic than antibodies</span></li>
+ <li><span>Can be attached to nanoparticles/polymers</span></li>
</ul>
- <ol>
- <ol>
- <li><span>Testing of PSMA promoter</span><span></span></li>
- <ul>
- <li><span>Evaluate the activity of PSMA promoter in different cell lines</span></li>
- <li><span>GFP (commonly used and known to work) as reporter gene</span></li>
- </ul>
- </ol>
- </ol>
-
- <ul>
- <li><strong><strong>Plasmid A: pENTR1A-PSMA-GFP</strong><span></span></strong></li>
- </ul>
-
- <ol>
- <ol>
- <ul>
- <li><span>Transfected to cancer and non-cancerous cell lines</span><span></span></li>
- </ul>
- <li><span>Testing of Gluc reporter gene</span><span></span></li>
- <ul>
- <li><span>Measure luminescence given out by reaction w/ Gluc as substrate</span></li>
- </ul>
- </ol>
- </ol>
-
+ <li><span>Potential aptamer 1: EpDT3 </span><span></span><span>16</span></li>
<ul>
- <li><strong><strong>Plasmid B: pENTR1A-PSMA-Gluc</strong></strong></li>
+ <li><span>Binds to EpCAM (epithelial cell adhesion molecule, overexpressed in some tumour cells) </span><span>17</span></li>
+ <li><span>ðŸ‘ðŸ»EpCAM is highly expressed in metastasized PCa cells → detect later stages of cancer </span><span>19</span></li>
+ <li><span>👎ðŸ»Still non-specific to PCa</span></li>
</ul>
-
- <ol>
- <ol>
+ <li><span>Dual aptamer design </span><span>23</span></li>
<ul>
- <li><span>Transfected to cancer and non-cancerous cell lines </span></li>
- <li><span>Testing of polymer delivery</span><span></span></li>
- <li><span>Make sure polymers can correctly transport plasmids to cancer cells </span></li>
+ <li><span>A10-3.2: PSMA +ve (but only 80% of PCa patients have PSMA)</span></li>
+ <li><span>DUP-1: PSMA -ve</span></li>
+ <li><span>Used in a lot of research previously</span></li>
+ <li><span>We can add a constitutive promoter inside our plasmid to express the reporter genes.</span></li>
</ul>
- </ol>
- </ol>
-
- <ul>
- <li><strong><strong>Polymer plasmid conjugate X:</strong></strong></li>
</ul>
<ul>
- <li><strong>PAMAM polymer</strong></li>
- </ul>
- <ul>
- <li><strong>A10-3.2 & DUP-1 aptamers</strong></li>
- </ul>
- <ul>
- <li><strong>Plasmid C: PB-Gluc</strong></li>
- </ul>
-
- <ol>
- <ol>
- <li><span>Testing of killing function</span></li>
+ <li><span>PD-L1 nanobody</span></li>
<ul>
- <li><span>Confirm that Bax gene can kill cancer cells </span></li>
- </ul>
- </ol>
- </ol>
-
+ <li><span>Normal cancer cells: have PD-L1 to “block†attacks from T-cells</span></li>
+ <li><span>PD-L1 nanobody: binds to PD-L1 so that cancer cells have no way to block attacks</span></li>
+ <li><span>Problem:</span></li>
<ul>
- <li><strong><strong>Polymer plasmid conjugate Y:</strong></strong></li>
+ <li><span>requires immune cells to kill</span></li>
+ <li><span>At later stages, immune system may be compromised</span></li>
+ <li><span>Cannot kill cancer cells effectively</span></li>
</ul>
- <ul>
- <li><strong>PAMAM polymer</strong></li>
</ul>
+ <li><span>Bax gene</span><span>(2./2.1)</span></li>
<ul>
- <li><strong>A10-3.2 & DUP-1 aptamers</strong></li>
+ <li><span>BCL2 associated X, apoptosis regulator</span></li>
+ <li><span>If Bax protein is transcribed, it promotes apoptosis (type of programmed cell death) in cancer cells</span></li>
</ul>
- <ul>
- <li><strong>Plasmid D: PB-Gluc-Bax</strong></li>
+ <li><span>Bax has been chosen</span></li>
</ul>
-
-
- </div>
- <div style="
- background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2410-1.png);
+ </div>
+
+ <div>
+ <div style="position:relative;">
+ <img src="https://static.igem.wiki/teams/5441/notebook/img-2521-1.png" style="width: 600px">
+ <a href="#Apr Week 1"><img src="https://static.igem.wiki/teams/5441/notebook/img-2522-1.png" style="width: min(460px, 80%); position: absolute; top: min(255px, 28.5%); left: min(50px, 10%)"></a>
+ <a href="#Apr Week 2"><img src="https://static.igem.wiki/teams/5441/notebook/img-2523-1.png" style="width: min(440px, 79%); position: absolute; top: min(330px, 36.5%); left: min(40px, 8%)"></a>
+ <a href="#Apr Week 3"><img src="https://static.igem.wiki/teams/5441/notebook/img-2524-1.png" style="width: min(420px, 79%); position: absolute; top: min(380px, 49.5%); left: min(55px, 10%)"></a>
+ <a href="#Apr Week 4"><img src="https://static.igem.wiki/teams/5441/notebook/img-2525-2.png" style="width: min(420px, 79%); position: absolute; top: min(480px, 57.5%); left: min(75px, 10%)"></a>
+ </div>
+ </div>
+ <div style="
+ background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
background-size: 100% 100%;
padding: 7%;
- padding-top: min(30%,180px);
-">
+ padding-top:max(10%,40px);position:relative;margin-top:70px
+"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2584-1.png" style="position:absolute; width: min(40%, 300px);left: 30%; top: -20px">
+ <a name="Apr Week 1"></a>
+ <h5><span>Week 1: Formulation of initial plan</span></h5>
+ <ol>
+ <li><span>Construction of plasmids</span></li>
+ <li><span>Purchase of A and B separately</span></li>
+ </ol>
+ <ol>
+ <li><span>A plasmid </span><strong>containing our gene</strong><span> that is </span><strong>non-mammalian</strong><span>; and</span></li>
+ <li><span>Another plasmid that is </span><strong>mammalian </strong><span>but </span><strong>lacks any promoter genes</strong><span> (pENTR1A)</span></li>
+ </ol>
+ <ul>
+ <li><span>A and B will be digested, then ligated together using </span><strong>BamHI </strong><span>and </span><strong>HindIII </strong><span>enzymes</span></li>
+ <li><span>Colony PCR → confirm size of plasmids are correct 1.e</span></li>
+ </ul>
+ <ol>
+ <ol>
+ <li><span>Testing of PSMA promoter</span><span></span></li>
+ <ul>
+ <li><span>Evaluate the activity of PSMA promoter in different cell lines</span></li>
+ <li><span>GFP (commonly used and known to work) as reporter gene</span></li>
+ </ul>
+ </ol>
+ </ol>
+
+ <ul>
+ <li><strong><strong>Plasmid A: pENTR1A-PSMA-GFP</strong><span></span></strong></li>
+ </ul>
+
+ <ol>
+ <ol>
+ <ul>
+ <li><span>Transfected to cancer and non-cancerous cell lines</span><span></span></li>
+ </ul>
+ <li><span>Testing of Gluc reporter gene</span><span></span></li>
+ <ul>
+ <li><span>Measure luminescence given out by reaction w/ Gluc as substrate</span></li>
+ </ul>
+ </ol>
+ </ol>
+
+ <ul>
+ <li><strong><strong>Plasmid B: pENTR1A-PSMA-Gluc</strong></strong></li>
+ </ul>
+
+ <ol>
+ <ol>
+ <ul>
+ <li><span>Transfected to cancer and non-cancerous cell lines </span></li>
+ <li><span>Testing of polymer delivery</span><span></span></li>
+ <li><span>Make sure polymers can correctly transport plasmids to cancer cells </span></li>
+ </ul>
+ </ol>
+ </ol>
+
+ <ul>
+ <li><strong><strong>Polymer plasmid conjugate X:</strong></strong></li>
+ </ul>
+ <ul>
+ <li><strong>PAMAM polymer</strong></li>
+ </ul>
+ <ul>
+ <li><strong>A10-3.2 & DUP-1 aptamers</strong></li>
+ </ul>
+ <ul>
+ <li><strong>Plasmid C: PB-Gluc</strong></li>
+ </ul>
+
+ <ol>
+ <ol>
+ <li><span>Testing of killing function</span></li>
+ <ul>
+ <li><span>Confirm that Bax gene can kill cancer cells </span></li>
+ </ul>
+ </ol>
+ </ol>
+
+ <ul>
+ <li><strong><strong>Polymer plasmid conjugate Y:</strong></strong></li>
+ </ul>
+ <ul>
+ <li><strong>PAMAM polymer</strong></li>
+ </ul>
+ <ul>
+ <li><strong>A10-3.2 & DUP-1 aptamers</strong></li>
+ </ul>
+ <ul>
+ <li><strong>Plasmid D: PB-Gluc-Bax</strong></li>
+ </ul>
+
+ </div>
+ <div style="
+ background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
+ background-size: 100% 100%;
+ padding: 7%;
+ padding-top:max(10%,40px);position:relative;margin-top:70px
+"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2585-1.png" style="position:absolute; width: min(40%, 300px);left: 30%; top: -20px">
<a name="Apr Week 2"></a>
- <h5><span>Week 2: Figuring out protocols used</span></h5>
+<h5><span>Week 2: Figuring out protocols used</span></h5>
+<ol>
+ <li><span>Plasmid construction</span>
<ol>
- <li><span>Plasmid construction</span>
+ <li><span>Plasmids in powder form: </span><strong>transformed </strong><span>into DH5a</span></li>
+ <li><span>Colonies are picked → plasmids </span><strong>extracted</strong></li>
+ <li><strong>Restriction digestion: </strong><span>linearise genes (BamHI and HindIII)</span></li>
+ <li><strong>Gel electrophoresis</strong><span>: visualise the results of digestion</span><span></span></li>
+ <li><strong>Gel purification</strong><span>: obtain the digested plasmids</span></li>
+ <li><strong>Ligation: </strong><span>stick two genes together</span></li>
+ <li><strong>Colony PCR</strong><span>: confirm that ligated genes have the correct size</span></li>
+ </ol>
+ </li>
+ <li><span>Polymers</span>
+ <ol>
+ <li><span>PEG is attached to PAMAM to form </span><strong>PAMAM-PEG</strong><span> conjugate</span></li>
+ <li><strong>Aptamers </strong><span>are attached to PAMAM-PEG </span><span></span></li>
+ <li><strong>BODIPY</strong><span> are introduced to some of the PAMAM-PEG</span>
<ol>
- <li><span>Plasmids in powder form: </span><strong>transformed </strong><span>into DH5a</span></li>
- <li><span>Colonies are picked → plasmids </span><strong>extracted</strong></li>
- <li><strong>Restriction digestion: </strong><span>linearise genes (BamHI and HindIII)</span></li>
- <li><strong>Gel electrophoresis</strong><span>: visualise the results of digestion</span><span></span></li>
- <li><strong>Gel purification</strong><span>: obtain the digested plasmids</span></li>
- <li><strong>Ligation: </strong><span>stick two genes together</span></li>
- <li><strong>Colony PCR</strong><span>: confirm that ligated genes have the correct size</span></li>
+ <li><span>Microscopes are used to view BODIPY </span><strong>fluorescence</strong></li>
+ <li><span>Confirm that aptamers can bring plasmids to cells</span></li>
</ol>
</li>
- <li><span>Polymers</span>
+ <li><strong>Plasmids C & D </strong><span>are introduced to other PAMAM-PEG</span></li>
+ <li><strong>Polymer-plasmid conjugates </strong><span>are mixed with cells</span>
<ol>
- <li><span>PEG is attached to PAMAM to form </span><strong>PAMAM-PEG</strong><span> conjugate</span></li>
- <li><strong>Aptamers </strong><span>are attached to PAMAM-PEG </span><span></span></li>
- <li><strong>BODIPY</strong><span> are introduced to some of the PAMAM-PEG</span>
- <ol>
- <li><span>Microscopes are used to view BODIPY </span><strong>fluorescence</strong></li>
- <li><span>Confirm that aptamers can bring plasmids to cells</span></li>
- </ol>
- </li>
- <li><strong>Plasmids C & D </strong><span>are introduced to other PAMAM-PEG</span></li>
- <li><strong>Polymer-plasmid conjugates </strong><span>are mixed with cells</span>
- <ol>
- <li><span>MTT Assay are performed to quantify the death of cancer cells (required absorbance measurement)</span></li>
- <li><span>Luminescence of Gluc are detected</span></li>
- </ol>
- </li>
+ <li><span>MTT Assay are performed to quantify the death of cancer cells (required absorbance measurement)</span></li>
+ <li><span>Luminescence of Gluc are detected</span></li>
</ol>
</li>
</ol>
- </div>
+ </li>
+</ol>
+ </div>
+
<div style="
- background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2411-1.png);
+ background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
background-size: 100% 100%;
padding: 7%;
- padding-top: min(30%,180px);
-">
+ padding-top:max(10%,40px);position:relative;margin-top:70px
+"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2589-1.png" style="position:absolute; width: min(40%, 300px);left: 30%; top: -20px">
+
<a name="Apr Week 3">
</a><h5><span>Week 3: Required equipment and apparatus</span></h5>
@@ -323,11 +321,12 @@ day for your project.{% endblock %}
</ul>
</div>
<div style="
- background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2412-1.png);
+ background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
background-size: 100% 100%;
padding: 7%;
- padding-top: min(30%,180px);
-">
+ padding-top:max(10%,40px);position:relative;margin-top:70px
+"> <img src="https://static.igem.wiki/teams/5441/notebook/img-2587-1.png" style="position:absolute; width: min(40%, 300px);left: 30%; top: -20px">
+
<a name="Apr Week 4">
</a><h5><span>Week 4: Evaluation of initial plan</span></h5>
@@ -339,80 +338,60 @@ day for your project.{% endblock %}
<li><span>🔜Polymers: the technical difficulty is too high</span></li>
</ul>
</div>
-
+ <a name="May"></a>
<div>
<div style="position:relative;">
<img src="https://static.igem.wiki/teams/5441/notebook/sample-1.png" style="width: 600px">
</div>
</div>
- <a name="May"></a>
- <h2>May: Purchasing of materials</h2>
+
- <div>
- <div style="position:relative;">
- <img src="https://static.igem.wiki/teams/5441/notebook/img-2576-1.png" style="width: 600px">
+ <a name="July-September"></a>
+ <div class ="row mt-4">
+ <div class = "col-lg-6">
+ <div style="position:relative;">
+ <img src="https://static.igem.wiki/teams/5441/notebook/img-2576-1.png">
+ </div>
</div>
- <div style="position:relative;">
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+ <img src="https://static.igem.wiki/teams/5441/notebook/img-2579-1.png">
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- <img src="https://static.igem.wiki/teams/5441/notebook/img-2577-1.png" style="width: 600px">
+ </div>
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+ <div class = "col-lg-6">
+ <div style="position:relative;">
+ <img src="https://static.igem.wiki/teams/5441/notebook/img-2578-1.png">
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+ <div style="position:relative;">
+ <img src="https://static.igem.wiki/teams/5441/notebook/img-2580-1.png">
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+ <div style="position:relative;">
+ <img src="https://static.igem.wiki/teams/5441/notebook/img-2577-1.png" style="width: 600px">
+ </div>
- <a name="July-September"></a>
- <h2>July - Sep: Wet and Dry lab</h2>
- <p><span>Success:✅</span></p>
- <p><span>Fail: âŒ</span></p>
- <p><span>Pending: ⌛</span></p>
- <div>
- <h5><span>July 1 ⌛</span></h5>
- <ul>
- <li><span>Prepare agar plates with kanamycin added</span></li>
- </ul>
- <h5><span>July 4 ✅</span></h5>
- <ul>
- <li><span>Transformed PB-Gluc-Bax and PSMA-GFP</span>
- <ul>
- <li><span>Colonies were seen</span></li>
- </ul></li>
- <li><span>Prepared cell culture mediumssssssssss</span></li>
- </ul>
- <h5><span>July 5 ✅</span></h5>
- <ul>
- <li><span>Transformed PSMA-Gluc</span>
- <ul>
- <li><span>Colonies were seen but may be contaminated</span></li>
- </ul></li>
- <li><span>Streaked PENTRIA (in bacterial stab) onto agar plate</span>
- <ul>
- <li><span>Colonies were seen but were small</span></li>
- </ul></li>
- <li><span>Prepared cell culture medium</span></li>
- </ul>
- <h5><span>July 6 âŒ</span></h5>
- <ul>
- <li><span>Picked colony and put it in a shaking incubator</span></li>
- <li><span>Extracted plasmid</span>
- <ul>
- <li><span>some samples don’t have a noticeable pellet</span></li>
- <li><span>maybe be due to low rotation speed of shaking incubator</span></li>
- </ul></li>
- <li><span>Accidentally added glycerol into plasmid to create stock → need to pick colony again</span></li>
- </ul>
- <img src="https://static.igem.wiki/teams/5441/notebook/img-2579-1.png">
- <img src="https://static.igem.wiki/teams/5441/notebook/img-2580-1.png">
+ </div>
+
+
+
+
<h5>July-September Journal</h5>
<p>Click <a href="https://static.igem.wiki/teams/5441/notebook/wet-lab-journal-compressed-1.pdf" download="">Here</a> to download PDF</p>
<embed src="https://static.igem.wiki/teams/5441/notebook/wet-lab-journal-compressed-1.pdf" type="application/pdf" style="width: 100%;height: 800px">
</div>
-</div>
- <div>
+ <div style="background-image: url(https://static.igem.wiki/teams/5441/notebook/img-2582-1.png);
+ background-size: 100% 100%;padding: 7%;">
<h2>Game Development Notebook</h2>
<h5><span>March</span></h5>
<ul>
--
GitLab