From 42df5723cc6e7fe4fb3f74e1871e780e120059a1 Mon Sep 17 00:00:00 2001
From: anvin-f <anvinfu@gmail.com>
Date: Wed, 2 Oct 2024 18:58:49 +0800
Subject: [PATCH] ws
---
wiki/pages/contribution.html | 4 ++--
wiki/pages/engineering.html | 8 ++++----
wiki/pages/human-practices.html | 16 ++++++++--------
wiki/pages/parts.html | 2 +-
4 files changed, 15 insertions(+), 15 deletions(-)
diff --git a/wiki/pages/contribution.html b/wiki/pages/contribution.html
index 7a77dbe..9b9d990 100644
--- a/wiki/pages/contribution.html
+++ b/wiki/pages/contribution.html
@@ -16,7 +16,7 @@ endblock %}
<p><span>Providing five tips to encourage others to have prostate cancer screening, along with some cancer facts, our handouts aim to </span><strong>raise awareness of prostate cancer</strong><span>. To </span><strong>maximize accessibility</strong><span> and </span><strong>engage a wider audience</strong><span>, handouts are available in both Chinese and English, both online and in reality.</span></p>
-<p><span>Apart from these, we have also documented some composite parts on the registry page, which are PSMA_GFP (BBa_K5441010), PSMA_GluC (BBa_K5441012) and Pb_GluC_BAX (BBa_K5441016). For details, please refer to the </span><a href = "/parts">parts page</a><span>.</span></p>
+<p><span>Apart from these, we have also documented some composite parts on the registry page, which are PSMA_GFP (BBa_K5441010), PSMA_GluC (BBa_K5441012) and Pb_GluC_BAX (BBa_K5441016). For details, please refer to the </span><a href = "parts">parts page</a><span>.</span></p>
-<p><span>Moreover, we have documented our troubleshooting process in plasmid construction. For more, please refer to the </span><a href = "/engineering">engineering page</a><span> and </span><a href = "/notebook">notebook page</a><span>.</span></p>
+<p><span>Moreover, we have documented our troubleshooting process in plasmid construction. For more, please refer to the </span><a href = "engineering">engineering page</a><span> and </span><a href = "notebook">notebook page</a><span>.</span></p>
{% endblock %}
\ No newline at end of file
diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html
index 7a107f3..1a4e157 100644
--- a/wiki/pages/engineering.html
+++ b/wiki/pages/engineering.html
@@ -244,11 +244,11 @@ endblock %}
<a name = "design1"></a>
<h5><span>Design</span></h5>
- <p><span>Based on the research papers by Vlachostergios et al. (2021), we </span><strong>chose prostate-specific membrane antigen, PSMA</strong><span>, as the biomarker for our project. PSMA is a protein found on the surface of prostate cancer cells discovered in recent years. It is one of the newest and most accurate biomarkers used in the detection and treatment of prostate cancer. (For more, please refer to the </span><a href = "/description">history of prostate cancer</a><span> on the description page). Given the fact that there is a high frequency of PSMA expression in all stages of prostate cancer (Minner et al., 2010; Tsourlakis et al., 2015), it has a higher correlation for prostate cancer than PSA, another biomarker commonly used.</span></p>
- <p><span>Based on the experience shared by the iGEM team last year, (referring to our</span><a href = "/human-practices"> integrated human practice</a><span>) protein purification typically results in a lower antibody yield, which makes it hard to verify the result. Apart from that, the concentration of PSMA in patients’ urine was unknown, making it tough to know the threshold value for detection. Thus, we decided against constructing a protein. Instead, we utilised </span><strong>another pathway with a similar concept to detect prostate cancer</strong><span> this year.</span></p>
+ <p><span>Based on the research papers by Vlachostergios et al. (2021), we </span><strong>chose prostate-specific membrane antigen, PSMA</strong><span>, as the biomarker for our project. PSMA is a protein found on the surface of prostate cancer cells discovered in recent years. It is one of the newest and most accurate biomarkers used in the detection and treatment of prostate cancer. (For more, please refer to the </span><a href = "description">history of prostate cancer</a><span> on the description page). Given the fact that there is a high frequency of PSMA expression in all stages of prostate cancer (Minner et al., 2010; Tsourlakis et al., 2015), it has a higher correlation for prostate cancer than PSA, another biomarker commonly used.</span></p>
+ <p><span>Based on the experience shared by the iGEM team last year, (referring to our</span><a href = "human-practices"> integrated human practice</a><span>) protein purification typically results in a lower antibody yield, which makes it hard to verify the result. Apart from that, the concentration of PSMA in patients’ urine was unknown, making it tough to know the threshold value for detection. Thus, we decided against constructing a protein. Instead, we utilised </span><strong>another pathway with a similar concept to detect prostate cancer</strong><span> this year.</span></p>
<p><span>The pathway we used to </span><strong>detect prostate cancer uses plasmids, containing PSMA promoters</strong><span>, which will be transported to the cells in the prostate gland. To produce PSMA, prostate cancer (PCa) cells contain substances that activate the promoter in its copy of the organism gene, which allows the transcription of PSMA to be facilitated. The same substance </span><strong>activates the PSMA promoters in our plasmid and transcribes our required genes to detect and kill cancer cells.</strong></p>
<p><span>At first, green fluorescent protein (GFP), a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range, stood out to our eyes. It was a commonly used gene with easy-to-record results, which can be </span><strong>easily excited using blue light and observed </strong><span>under an inverted microscope. Utilising these characteristics, we can use GFP to</span><strong> evaluate the proficiency of the promoter</strong><span> of our chosen biomarker, </span><strong>PSMA</strong><span>.</span></p>
- <p><span>For more information regarding the design of the plasmid, please refer to our </span><a href = "/description">Description</a><span> page.</span></p>
+ <p><span>For more information regarding the design of the plasmid, please refer to our </span><a href = "description">Description</a><span> page.</span></p>
<p><span>Aim: to build a plasmid (pENTR1A-PSMA-GFP) that allows us to detect PSMA-positive prostate cancer cells by measuring the fluorescence by GFP.</span></p>
<br>
@@ -268,7 +268,7 @@ endblock %}
<p><span>We collected the cell media of the cancer cells containing transfected plasmids, which were then photographed with an inverted microscope. At first, we recorded a bright image with a lot of light-emitting spots. However, it was suspected to be a false positive result, as the spots are larger than the cells themselves.</span></p>
<h6><span>First attempt evaluation</span></h6>
- <p><span>We have discussed the results with Dr Yu, who has extensive experience in GFP analysis. (For more, please refer to our </span><a href = "/human-practices">integrated human practice</a><span> page)</span></p>
+ <p><span>We have discussed the results with Dr Yu, who has extensive experience in GFP analysis. (For more, please refer to our </span><a href = "human-practices">integrated human practice</a><span> page)</span></p>
<p><span>Possible reasons:</span></p>
<ol>
<li><span>Most of the spots are a result of </span><strong>autofluorescence </strong><span>from the cells themselves.</span></li>
diff --git a/wiki/pages/human-practices.html b/wiki/pages/human-practices.html
index 58ea119..2f5755e 100644
--- a/wiki/pages/human-practices.html
+++ b/wiki/pages/human-practices.html
@@ -33,7 +33,7 @@
<p><span>Special Thanks to</span></p>
<p><span>Miss Ng Shuk Yee (Cell Culture Expert), experience in commercial laboratory,</span></p>
<p><a href="#Dr Yu from G.T. (Ellen Yeung) College"><span>Dr Yu, experience in instrumental testing on GFP</span></a></p>
- <p><a href="#Mr. Lau from Tin Ka Ping College"><span>Mr Lau, experience in troubleshooting of experimental steps</span></a></p>
+ <p><a href="#Mr. Lau from Yan Oi Tong Tin Ka Ping Secondary School"><span>Mr Lau, experience in troubleshooting of experimental steps</span></a></p>
<h2><span>Organisation:</span></h2>
@@ -58,7 +58,7 @@
<img src="https://static.igem.wiki/teams/5441/ihp/flow2.png" style="aspect-ratio:628/700">
</div>
<ul style="width:33%">
- <li>- <a href="#Mr. Lau from Tin Ka Ping College">Interview with Paul Lau</a></li>
+ <li>- <a href="#Mr. Lau from Yan Oi Tong Tin Ka Ping Secondary School">Interview with Paul Lau</a></li>
<li>- <a href="#Goethe University Frankfurt">Collaboration with Goethe Frankfurt University</a></li>
<li>- <a href="#The Hong Kong University of Science and Technology">Collaboration with HKUST</a></li>
</ul>
@@ -71,7 +71,7 @@
<img src="https://static.igem.wiki/teams/5441/ihp/flowl.png" style="aspect-ratio:628/700">
</div>
<ul style="width:33%">
- <li>- <a href="#Mr. Lau from Tin Ka Ping College">Consultation from Paul Lau</a></li>
+ <li>- <a href="#Mr. Lau from Yan Oi Tong Tin Ka Ping Secondary School">Consultation from Paul Lau</a></li>
<li>- <a href="#Dr Yu from G.T. (Ellen Yeung) College">Consult with Dr Yu</a></li>
</ul>
<div style="width:33%;">
@@ -128,9 +128,9 @@
</a>
</li>
<li>
- <a href="#Mr. Lau from Tin Ka Ping College">
+ <a href="#Mr. Lau from Yan Oi Tong Tin Ka Ping Secondary School">
<div class="date">Apr</div>
- <div class="eve">Mr. Lau from Tin Ka Ping College
+ <div class="eve">Mr. Lau from Yan Oi Tong Tin Ka Ping Secondary School
</div>
</a>
</li>
@@ -275,11 +275,11 @@
padding: 20%;
width: 100%;
">
- <a name="Mr. Lau from Tin Ka Ping College"></a>
+ <a name="Mr. Lau from Yan Oi Tong Tin Ka Ping Secondary School"></a>
<br>
- <h2>Mr. Lau from Tin Ka Ping College</h2>
+ <h2>Mr. Lau from Yan Oi Tong Tin Ka Ping Secondary School</h2>
- <p><span>We are honoured to receive generous assistance from </span><strong>Mr. Paul Lau</strong><span>, an experienced teacher at </span><strong>Tin Ka Ping College</strong><span>. Mr. Lau had a leading role in the first iGEM team from Hong Kong. Working in close cooperation with such a pioneer has offered us invaluable insights and suggestions about our projection procedures and guidance on our experimental approach. Mr Lau contributed to the </span><span style="color: orange;">building</span><span> step of our engineering cycle by training us in the biology field through conducting a lecturing talk, he has also taught us some </span><strong>experimental skills and guided us how to do troubleshooting in gel purification and ligation</strong><span>, which greatly assisted us in facilitating the progress and process of our experiment.</span></p>
+ <p><span>We are honoured to receive generous assistance from </span><strong>Mr. Paul Lau</strong><span>, an experienced teacher at </span><strong>Yan Oi Tong Tin Ka Ping Secondary School</strong><span>. Mr. Lau had a leading role in the first iGEM team from Hong Kong. Working in close cooperation with such a pioneer has offered us invaluable insights and suggestions about our projection procedures and guidance on our experimental approach. Mr Lau contributed to the </span><span style="color: orange;">building</span><span> step of our engineering cycle by training us in the biology field through conducting a lecturing talk, he has also taught us some </span><strong>experimental skills and guided us how to do troubleshooting in gel purification and ligation</strong><span>, which greatly assisted us in facilitating the progress and process of our experiment.</span></p>
<p><span>Mr Lau contributes to the </span><span style="color: orange;">build</span><strong> and </strong><span style="color: orange;">testing</span><strong> stage</strong><span> of our research work. We have done a lot of literature review to </span><strong>decide the multicistronic plasmid and discussed the feasibility of the functional role of the gene</strong><span>. During the </span><span style="color: orange;">testing</span><span> stage</span><span>, we have revised substantially </span><strong>lab techniques for cloning the gene</strong><span>.</span></p>
<h5>Learn:</h5><p><span>We </span><strong>amended our protocols</strong><span>, gave Mr. Lau feedback on the challenges we had overcome and discussed our troubleshooting process.</span></p>
diff --git a/wiki/pages/parts.html b/wiki/pages/parts.html
index d9e136a..c0b218e 100644
--- a/wiki/pages/parts.html
+++ b/wiki/pages/parts.html
@@ -8,7 +8,7 @@
<p><span>This year, our project will compose for 3 plasmids, pENTR1A-PSMA-GFP, pENTR1A-PSMA-GluC and pENTR1A-Pb-GluC-BAX. Here are the basic parts and composite parts we used in the experiment:</span></p>
-<p><span>For more information of the plasmid and experiments, please refer to the </span><a href = "/design">experimental design page</a><span>.</span></p>
+<p><span>For more information of the plasmid and experiments, please refer to the </span><a href = "design">experimental design page</a><span>.</span></p>
<br>
<p><span>Table 1. Basic Parts</span></p>
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GitLab