diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html
index 08933bec31194e31860780fe9f2c24f3529ad41e..bbf08e4a648e19e0a40aa7f9ee2667e629b47287 100644
--- a/wiki/pages/engineering.html
+++ b/wiki/pages/engineering.html
@@ -183,7 +183,7 @@
             <div class="h1">Cycle 4: Enzyme-constrained model predicts novel targets</div>
             <h3><b>Design</b></h3>
             <p>The intracellular metabolic activities of microorganisms are complex, and it is difficult to systematically understand their regulatory mechanisms and efficiently obtain the required phenotypes by a single research method. Genome-scale metabolic model (GSMM) is a mathematical model used to describe the relationship between genes, proteins, and responses, and has been widely used to analyze network properties, predict cell phenotypes, guide strain design, and analyze interactions. Based on GSMM, the enzyme-constrained model constructed by integrating large-scale enzyme kinetics and proteomics data has more accurate phenotypic prediction ability. </p >
-            <p>In our experiments, in order to improve the yield of 5-ALA, we used an enzyme-constrained model to simulate the effects of the following strategies on the target product from a global perspective while enzymatically modifying ALAS, combined with different genetic modification methods: 1) eliminate the competition pathway; 2) strengthen the synthesis of pathway-critical genes; 3) eliminate feedback inhibition; 4) optimize cofactors. This method is helpful to screen and combine different metabolic modification targets and guide experimental design, which not only realizes the rational design of strain modification, improves the efficiency of metabolic engineering, but also comprehensively considers the impact of genetic perturbation on microbial intracellular metabolism, so as to achieve precise metabolic flux regulation. We used the enzyme-constrained model that had been constructed in <i>E. coli BL21(DE3)</i> as the initial model, and since the initial model did not include relevant reactions regarding the synthesis ec_iECBD_1354 of 5-ALA by the C4 pathway, we introduced the synthesis pathway of 5-ALA by the C4 pathway into the original model. </p > 
+            <p>In our experiments, in order to improve the yield of 5-ALA, we used an enzyme-constrained model to simulate the effects of the following strategies on the target product from a global perspective while enzymatically modifying ALAS, combined with different genetic modification methods: 1) eliminate the competition pathway; 2) strengthen the synthesis of pathway-critical genes; 3) eliminate feedback inhibition; 4) optimize cofactors. This method is helpful to screen and combine different metabolic modification targets and guide experimental design, which not only realizes the rational design of strain modification, improves the efficiency of metabolic engineering, but also comprehensively considers the impact of genetic perturbation on microbial intracellular metabolism, so as to achieve precise metabolic flux regulation. We used the enzyme-constrained model ec_iECBD_1354 [3] that had been constructed in <i>E. coli BL21(DE3)</i> as the initial model, and since the initial model did not include relevant reactions regarding the synthesis of 5-ALA by the C4 pathway, we introduced the synthesis pathway of 5-ALA by the C4 pathway into the original model. </p > 
               <p><a style="font-size: 15px;line-height: 1;">Succinyl-CoA[c] = >5-Amino-4-oxopentanoate[c]+ Coenzyme A[c] + CO2[c]</a></p>
                 <p>In addition, the transport and exchange reactions of 5-ALA are described:</p>
                 <p>Transport reaction: </p>
@@ -478,7 +478,7 @@
                 </tr>
               </tbody>
             </table>
-            <p>The ZZ-2 strain (transposon group 2 strain, whose 3rd target pta was inserted by the ALAS gene) was used as the chassis cell. For genes that need to be upregulated, we overexpressed the strong promoter pJ23119 with a highly efficient ribosomal binding site (RBS) sequence. The fermentation results are shown in the figure: </p >
+            <p>The ZZ-2 strain (transposon group 2 strain, whose 3rd target pta was inserted by the ALAS gene) was used as the chassis cell. For the genes that needed to be up-regulated, we replaced their original promoter and ribosome binding site (RBS) sequence genes with the strong promoter pJ23119 and the more efficient RBS sequence genes. The fermentation results are shown in the figure: </p >
             <div class="img-pagestyle" style="width:70%">
               <img src="https://static.igem.wiki/teams/5303/results/up.png">
             </div>
@@ -548,7 +548,7 @@
             <div class="h1">Reference</div>
             <p>1.	Strecker, J., et al., RNA-guided DNA insertion with CRISPR-associated transposases. Science, 2019. 365(6448): p. 48-53.</p>
             <p>2.	Lauritsen, I., et al., A versatile one-step CRISPR-Cas9 based approach to plasmid-curing. Microb Cell Fact, 2017. 16(1):135.</p>
-            <p>3.	Cheng, Z., et al., Progress in metabolic engineering of microorganisms for the utilization of formate. Synth. Biol, 2023. 4(4): p. 756-778.</p>
+            <p>3.	Zhang, Z.X., et al., Developing a dynamic equilibrium system in <i>Escherichia coli</i> to improve the production of recombinant proteins. Appl Microbiol Biotechnol, 2022. 106(18): 6125-6137.</p>
             </div>
     </div>
   </div>